EMAGE mouse gene expression spatial database

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EMAGE:12453

Cep152 centrosomal protein 152 ( MGI:2139083)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011313. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011313_01	euxassay_011313_02	euxassay_011313_03	euxassay_011313_04

euxassay_011313_05	euxassay_011313_06	euxassay_011313_07	euxassay_011313_08	euxassay_011313_09

euxassay_011313_10	euxassay_011313_11	euxassay_011313_12	euxassay_011313_13	euxassay_011313_14

euxassay_011313_15	euxassay_011313_16	euxassay_011313_17	euxassay_011313_18	euxassay_011313_19

euxassay_011313_20	euxassay_011313_21	euxassay_011313_22	euxassay_011313_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12453_wholemount_strong.wlz

12453_wholemount_moderate.wlz

12453_wholemount_weak.wlz

12453_wholemount_possible.wlz

12453_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12453_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

humerus

moderate

regional

moderate expression: see section 04

femur

moderate

regional

moderate expression: see section 05 06 22

pituitary gland

moderate

single cell

moderate expression: see section 11 13 14

diencephalon roof plate

moderate

regional

moderate expression: see section 13 15

choroid invagination

moderate

regional

moderate expression: see section 07 08 11 12 16 17 18 19 20 21 weak expression: see section 09 10

metencephalon part of 4th ventricle choroid plexus

moderate

regional

moderate expression: see section 07 08 11 12 14 15 16 17 18 19 20 weak expression: see section 09 10

inner ear

strong

regional

strong expression: see section 03 04 05 06 20 21 22

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18 19 20 21

vomeronasal organ

strong

regional

strong expression: see section 13 16

pharynx epithelium

strong

regional

strong expression: see section 12 13 14 15 16

axial skeleton

moderate

regional

moderate expression: see section 09 12 13 14 16 17

basioccipital bone

moderate

regional

moderate expression: see section 06 17 18

scapula

moderate

regional

moderate expression: see section 04 22 weak expression: see section 23

pelvic girdle skeleton

moderate

regional

moderate expression: see section 20

axial skeleton tail region

moderate

regional

moderate expression: see section 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37551
Entity Detected:	Cep152, centrosomal protein 152 ( MGI:2139083)
Sequence:	sense strand is shown >T37551 AACTTGGAGAGGAGAACAGCAGGGTGGTTGAAGAATTAATAGAGGAGAACCATGACATGAAGAATAAACT GGAGGCACTGAGGGCCCTTTGCAGAACGCCACCACGGTCACTGTCTGCAGGAGCCGCTGAGAGTGCTGGG CCGTCATGCAGTAGGCAGGCCTTGGAAGAACTCCGCGGGCAGTACATTAAAGCTGTGAGAAAAATTAAAC GTGACATGCTTCGGTATATTCAGGAGAGCAAGGAAAGAGCTGCAGAGATGGTAAAAGCGGAAGTGTTGCG GGAACGGCAGGAAACTGCACGGAAGATGCGCAATTATTACCTGAGTTGTCTTCAGCAGATCTTGCAGGAT AATGGAAAAGAGGAGGGAGCTGAGAAAAAGATTATGAGTGCTGCTAGCAAACTCGCTACAATGGCGGAGT TGCTGGGGACGATCGCGGAGTCAGACTGCAGAGTCCGGTGTGCCCAGGCAGGCCGCTCAGTAGCACTTCC TTTGGCTTCAGAGATGCTGACGGGCACTGAAAGATCAGAAAGGAGTGGTGTGAATCACAATATTCCACAT TACGTTGAAAGCAAACCAAACAGTGGAAAGACCCTCCCCAGGAGTGTGTGTGAGCAGCTGCCTGGGAGGA AGGCTGCGCCCAGGTCACAGAGGCGGTTAGAGGAATCGAAGCACAGAGAGATGAGACCCATGGCTTCCAC AGCTTTGCCTTCAGACTGTCGCTGTGGGGATGCGAGTTGCAGGCACTCAGGCGTCTTGGCAAAGGATGTT GCTCCTGAATTTGTTCCGTGCCAGGGCGAGGGAGGCTTTGACTTGCACGAGAAGAGAGATGCACTTGGAG CTGGCTCTGAGCCGCTGCTCTATTCAGCTGCTCACTCCTTCCTTGGAGGTGCCGAAAAGAACTCTTCTCC TAGATGCATCTCAGAGTCCAGGCACACAACCCTGAGAAGTCCCAGTGAAATGCCAAGGTTAA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 77067. Forward Primer - name:077067_F_cDNA_AI851464, sequence:AACTTGGAGAGGAGAACAGCAG; Reverse Primer - name:077067_N_SP6_cDNA_AI851464, sequence:TTAACCTTGGCATTTCACTGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12451 same embryo
	EMAGE:12450 same embryo
	EMAGE:12452 same embryo
	EurExpress:euxassay_011313 same experiment
	MGI:4823813 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source