Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:12457

Fam89b family with sequence similarity 89, member B ( MGI:106595)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12457 EMAGE:12457 EMAGE:12457 EMAGE:12457 EMAGE:12457
"Pseudo-wholemount" of euxassay_011245. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011245_01 euxassay_011245_02 euxassay_011245_03 euxassay_011245_04
EMAGE:12457 EMAGE:12457 EMAGE:12457 EMAGE:12457 EMAGE:12457
euxassay_011245_05 euxassay_011245_06 euxassay_011245_07 euxassay_011245_08 euxassay_011245_09
EMAGE:12457 EMAGE:12457 EMAGE:12457 EMAGE:12457 EMAGE:12457
euxassay_011245_10 euxassay_011245_11 euxassay_011245_12 euxassay_011245_13 euxassay_011245_14
EMAGE:12457 EMAGE:12457 EMAGE:12457 EMAGE:12457 EMAGE:12457
euxassay_011245_15 euxassay_011245_16 euxassay_011245_17 euxassay_011245_18 euxassay_011245_19
EMAGE:12457 EMAGE:12457 EMAGE:12457 EMAGE:12457
euxassay_011245_20 euxassay_011245_21 euxassay_011245_22 euxassay_011245_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12457Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12457_wholemount_strong.wlz
12457_wholemount_moderate.wlz
12457_wholemount_weak.wlz
12457_wholemount_possible.wlz
12457_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12457_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
medulla oblongata basal plate mantle layer
moderate moderate
regionalmoderate expression: see section 09 10 15 16
facial vii ganglion
moderate moderate
regionalmoderate expression: see section 04 05 06 07 19 20 21
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 07 08 weak expression: see section 18
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22
vagus x ganglion
moderate moderate
regionalmoderate expression: see section 09 weak expression: see section 17
vestibulocochlear viii ganglion
moderate moderate
regionalmoderate expression: see section 07 08 weak expression: see section 17 18
trigeminal v nerve
moderate moderate
regionalmoderate expression: see section 09 10 16
neural retina
moderate moderate
regionalmoderate expression: see section 01 02 03 22 23
nasal cavity olfactory epithelium
weak weak
regionalweak expression: see section 09 10 11 14 15 16 17
vomeronasal organ
weak weak
regionalweak expression: see section 14
aortic valve
weak weak
regionalweak expression: see section 13 14
mitral valve
moderate moderate
regionalmoderate expression: see section 11 12
tricuspid valve
moderate moderate
regionalmoderate expression: see section 15 weak expression: see section 14
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6773
Entity Detected:Fam89b, family with sequence similarity 89, member B ( MGI:106595)
Sequence:sense strand is shown

>T6773
CCACGCGTCCGCTCCGGAGGCCGGGCCACGGAGAGCTGGTTCTCTGGGAAACAGCCCGCCGAAAACGTAA
GAGGGGTCCAGGCGGCATCGTGGTCCACGCCTGCCTGGCTATGAACGGGCTGCCCGCGACTGAGGCGCCG
GGCGGCGCGGGCTGCGCTCTGGCCGGGCTCCCGCCGCTACCGCGCGGCCTCAGCGGCCTTCTTAACGCTA
GTGGGGGCTCGTGGAGGGAGCTGGAGCGTGTCTACAGCCAACGGAGCCGCATCCACGACGAACTGAGTCG
TGCCGCCCGCGTCCCGGATGGTCCTCGCCATGCCGCTGGATCTGCTAACTCAGGTTCTGCAGCGGGCCCG
CGCCGCCCGGTTAACCTCGACTCTGCACTAGCCGCGCTGCGCAAGGAGATGGTGGGGCTGCGACAGCTGG
ATATGTCCCTGTTGTGCCAGCTGTGGGGCCTGTACGAGTCGATCCAGGACTACAAGCATCTGTGCCAAGA
CCTGAGCTTGTGCCAGGACCTGTCATCCTCTCTGCACTCGGACAGCTCCTACCCACCTGATGCCGGCCTG
TCTGATGACGAGGAGCCTCCTGATGCCAGCCTGCCCCCAGACCCTCCACCCCTCACCGTACCCCAGACTC
ACAATGCCCGTGACCAGTGGTTGCAGGATGCCTTCCAAATCAGCCTTTGAAAGCTGAGGTGGATAGGAAC
GCACCCATATAAAAGGCTCAAAACTTGCCCTTCAGCAAGTCCTTAGTCTACAGTGCCTGCTCTTTTACCG
GTACCACCTCACCTCACTTCAGGAGGGCAAGGCATTATACCCATCAGGCAAAGCGGTCAAGATTGCAGCC
TGTGCCCAGTGTCCTGGGCTTCCACTTCTCCCACATGTGTGCACCCTAGCTTGGCCAGCCCTTAGCCTGA
CTGTGGGGCCCAAAGCGTCTTGCACTCCCGTTTGCATCTCTGGGACTGGGATGAGTGCCTAGCTCCCATC
TCTTTTTCGTCTTTTGCTGCCTCTTGCAGCTGCTGGCTCAGCGGCATCCCTACCAGTGGCCCCAGGGGTT
CATTGCCTGGGACAAGGGCTCCAGAACCCACCTCTGTAGCCACCCACGACCAGAAAGGCTAAGGTCTTTA
TGCTGGATATTTAAGTTTAGCGGCCAGTTCCTGGGCGGGAAAATAAATAATTTCAGCATTGTAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3674980 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3674980 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12454 same embryo
 EMAGE:12455 same embryo
 EMAGE:12456 same embryo
 EurExpress:euxassay_011245 same experiment
 MGI:4826503 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS