EMAGE mouse gene expression spatial database

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EMAGE:12509

Tmtc2 transmembrane and tetratricopeptide repeat containing 2 ( MGI:1914057)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011308. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011308_01	euxassay_011308_02	euxassay_011308_03	euxassay_011308_04

euxassay_011308_05	euxassay_011308_06	euxassay_011308_07	euxassay_011308_08	euxassay_011308_09

euxassay_011308_10	euxassay_011308_11	euxassay_011308_12	euxassay_011308_13	euxassay_011308_14

euxassay_011308_15	euxassay_011308_16	euxassay_011308_17	euxassay_011308_18	euxassay_011308_19

euxassay_011308_20	euxassay_011308_21	euxassay_011308_22	euxassay_011308_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12509_wholemount_strong.wlz

12509_wholemount_moderate.wlz

12509_wholemount_weak.wlz

12509_wholemount_possible.wlz

12509_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12509_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper arm muscle

strong

regional

strong expression: see section 01 02 03 04 05 21 22

upper leg muscle

strong

regional

strong expression: see section 01 02 03 04 05 17 18 19 20 21 22 23

rib

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 14 15 16 17 18 19 20 21 22 23

diaphragm

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

hand

strong

regional

strong expression: see section 01 02 21 22

foot

strong

regional

strong expression: see section 01 15 16 17 18 19 20

tibia

strong

regional

strong expression: see section 23

lower leg rest of mesenchyme

strong

regional

strong expression: see section 01 02 19 20 21 22 23

upper leg rest of mesenchyme

strong

regional

strong expression: see section 01 02 20 21 22

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

brain

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

vibrissa

strong

regional

strong expression: see section 01 02 03 04 15 16 17 18 19 20

facial vii ganglion

strong

regional

strong expression: see section 04 05 06 20

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 07 08 18

trigeminal v ganglion

strong

regional

strong expression: see section 02 03 04 05 06 07 08 16 17 18 19 20 21

vagus x ganglion

strong

regional

strong expression: see section 08 17

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 05 06 07 16 17 18 19 20 21

spinal cord

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17 18

dorsal root ganglion

strong

regional

strong expression: see section 09 10 11 12 14 15 16 17 18 19

cornea

strong

regional

strong expression: see section 01

retina

strong

regional

strong expression: see section 01 19 20 21 22 23

anterior naris epithelium

strong

regional

strong expression: see section 07 08 09 10 11 12

external naris epithelium

strong

regional

strong expression: see section 07 08 12 13

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16

heart valve

strong

regional

strong expression: see section 10 11 12 13 14

esophagus

strong

regional

strong expression: see section 13 14

tongue muscle

strong

regional

strong expression: see section 07 08 09 10 11 12 13

stomach

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10

hindgut

strong

regional

strong expression: see section 11

midgut

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

mandible

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

maxilla

strong

regional

strong expression: see section 03 04 05 06 07 08 09 13 14 15 16 17 18 19

bladder

strong

regional

strong expression: see section 09 10 11 12 13

kidney pelvis

strong

regional

strong expression: see section 08 09

ureter

strong

regional

strong expression: see section 10 11 14 15 16

renal capsule

strong

regional

strong expression: see section 07 08 09 10 11 15 16 17 18 19 20

axial skeleton

strong

regional

strong expression: see section 13 14 15

clavicle

strong

regional

strong expression: see section 05 06 07 08 09 10 16 17 18 19 20 21 22

tail paraxial mesenchyme

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12

axial skeleton tail region

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9359
Entity Detected:	Tmtc2, transmembrane and tetratricopeptide repeat containing 2 ( MGI:1914057)
Sequence:	sense strand is shown >T9359 CCGCGACGAGAACCCCACGCGGGAGCTTTTGGAGTCCCCAGTCGCCGCTTCTCACTTCACTGGAGAGGGG TCCGGGAGGGAACCAATAAAGAGTGAATGTGCCGCAGCCGCGGTGGCGGCCGAGTTGCTGCGAACGGCCC CGGAGTCGTGCGCCCGGGAGGCGGCGAAGGGAGGCGGTTCGCCTCGTCCCTCCCACCCGCCCCTTTCTCT CTCGGTCTCTCTCTCTGTCTCTCCTTTCCTCCCGCTGGTCCCAGATCCTCGCTCTGTCCAGCCCTTTGGT CCCCCCTCTTGCACTCTTCTATCTCCGCAGTCCTTTACCTTAGGGCGTCCCAAAGTGCCATGGGTTAGGG TGGGGACCGCGAGCCGCGGGAAAAGGACAAGCCCCGATTCTCCGCCGGGGGGCGTGAAGCCGATCGCCGC TTGCCTCTCGCGGTTGTCGGGTATGGGGAGAGCGGTGTGAGCCCGCAACCCCCGGAGCACGCAGGAACAG CGGAGCCCGGCGCCCCGAGGAGGAGCCCGGGATCGGAAGAAGGAGAGGAGGGAGGACGAGGAGGCGGGCG GGGAAGCCGGGGAAAAGTGCAGCCAAGGAGGAGAAGGCGGCGGAAGGTGGGGATTGATGCTTCTGTTTTT TGTTGCCGCTGCTGCGCTCGCGCTGGGAGCCAAGCCGGAGGGAAGGCGGTGGAGAGATGATTGCAGAGTT GGTGAGCAGCGCTCTGGGGCTCGCCTTGTATCTCAACACCCTGAGTGCTGATTTCTGCTACGATGACAGC CGTGCTATCAAGACTAATCAAGACCTCCTGCCAGAAACCCCCTGGACACACATTTTCTACAATGACTTTT GGGGGACTCTTCTAACCCACAGCGGTAGCCACAAGTCCTACCGGCCACTCTGCACTCTCTCTTTTCGACT GAATCATGCCATTGGAGGGCTGAATCCCTGGAGCTATCACCTTGTCAATGTCCTGTTGCATGCGGCTGTC ACCGGCCTCTTCACACGCTTCTCCAAGGCCCTCCTTGGGGACGGATACTGGACCTTCATGGCTGGCTTGA TGTTTGCTTCACACCCCATCCACACGGAAGCGGTGGCGGGCATCGTGGGACGAGCAGATGTGGGCGCTAG TCTCTTTTTTCTCCTGTCCCTGCTCTGCTACATTAAGCACTGTTCTACAAGAGGCTACTCTGCCAGAACT TGGGGCTGGTTCCTGGGGACTGGATTGTGCGCTGGATGCAGCATGCTTTGGAAAGAGCAAGGAGTGACTG TCCTGGCAGTTTCAGCCGTTTATGACGTCTTTGTCTTCCACAGGCTAAAAATGAAGCAGATCCTGCCTAC CATTTACAAAAGGAAGAACTTGTCTCTTTTTCTCAGCATTAGCTTGTTAACATTCTGGGGGACCTGCCTT TTGGGTGCCCGGCTGTACTGGATGGGCAATAAACCACCAAGCTTCTCCAACTCCGACAACCCCGCGGCTG ACTCGGACAGCCTTTTGGCTCGCACGCTCACCTTCTTGTATTTACCTACCAAGAACCTCTGGCTATTGCT CTGCCCGGACACCCTGAGCTTTGATTGGTCCATGGATGCCGTGCCTCTTCTCAAAACAGTTTGTGATTGG AGAAATCTACACACTGTGGCCTTCTATTCTGGACTTCTCCTCCTTGCCTACTGTGGTTTGAAGAACCCAA GTTTAGAAGGAGAGTGCAACGGGAAAGCCTTGACAAATGGCAAGCAGAATGCAAATGGCCATAGCTGCCA CTCAGACGTGGAGTACCGGAACTCAGAAATGAAGCCCAGCTTTGCATCCAAAGTAGAGAATGGAATTAAA AATTGTGTACCACAGAGAACTCAGCTCCCGTCCACGGAGAACATTGTTATTCTGTCCTTATCTTTGTTGA TAATACCTTTTATTCCTGCCACGAACCTGTTTTTCTATGTCGGCTTTGTAATCGCAGAGCGAGTCTTATA TATTCCCAGTATGGGCTTCTGCCTTCTGATCACAGTGGGTGCCAGAGCCCTGTATGTCAAAGTACAAAAG AGGTTTCTCAAGAGCTTGGTTTTTTATGCTACTGCCACGTTAATTGTCTTCTACGGAGTCAAGACTGCGA TCAGGAACGGAGACTGGCAGAACGAGGAGATGCTGTATAGATCAGGGATCAAAGTGAACCCAGCCAAAGC ATGGGGTAACCTTGGAAATGTTCTGAAGAGTCAGAGTAAAATCTCTGAAGCTGAAAGCGCCTACAGAAAT GCTCTGTTCTACCGCAGCAACATGGCTGACATGCTGTATAATCTAGGGTTGCTGCTCCAAGAGAACAGCA GGTTTGCAGAAGCTCTTCACTATTACAAACTGGCCATTGGGAGCAGGCCTACCCTGGCTTCTGCCTATTT AAACACCGGCATTATTCTCATGAACCAAGGGAAGACAGAAGAAGCCCGGCGGACATTCCTCAAGTGTTCT GAGATTCCAGATGAGAACCTGAAGGATCCTCATGCACATAAGAGTTCTGTTACCAGCTGTCTGTACAACC TCGGGAAACTCTATCACGAGCAAGGACGCTATGAGGAAGCCCTGAGTGTATATAGGGAAGCAATTCAGAA GATGCCAAGGCACTTTGCCCCTCAGAGCCTGTACAACATGATGGGTGAAGCCTACATGAGACTGAGTAAG CTTCCTGAAGCTGAGCATTGGTACATGGAATCACTGAGATCCAAGACCGATCACATCCCTGCTCATCTCA CCTACGGGAAGCTGCTCGCCTTAACAGGTCGTAAGAGTGAGGCTGAAAAGTTCTTCTTGAAGGCTATTGA GCTGGATCCTACCAAAGGAAACTGTTACATGCACTATGGTCAGTTTCTCCTAGAAGAGTCTCGCCTCACA GAAGCAGCAGAGATGGCAAAGAAAGCAGCTGAGCTGGACAACACAGAGTTCGATGTCGTCTTCAATGCTG CCCATATGCTCAGACAAGCAAGCCTCAATGAAGCCGCTGAGAAGTATTACGATCTGGCAGCAAGACTGAG ACCTAATTACCCTGCTGCTCTCATGAACCTAGGGGCCATTCTGCATCTCAATGGGCGACTGCAGAAGGCT GAGGCCAACTACCTGAGGGCCCTGCAACTCAAGCCAGATGATGTCATCACTCAGTCTAATCTCCGCAAGC TGTGGAACATCATGGAAAAGCAAGGTTTGAAGACCTCCAAGACCTGACACAGGAGAACTCCCCTCCTCCT CCTCCGCTTGTCAGAGCTGGCTTCATTAACAGACAGAACTTCCCAGGAGTGCTGTGATAAAAGCTCAAGG GCGACTTCAAGCCCAGGACAAAGGTCACTATGGTTACTGCCACTGCTGGGAGAATCCACTTTGCTATGGG CACAGCCCTTAACACCAAAAAGGGGAACATTGAGACCCTCTGGGAGGGTGTAAACTGTTACCCGTAGACT GTAAACCAGAGCACCTAAGAGGATTTCGGAGAGTTCCTAGAAAGTAGAGGGGTCTCATTTGCAGATGTCC ACTTTGGAGGGAGAATTTGAAATTCTATTGTTTCTTAAACACTGTGAGAGGAAGTCAGAGAGCCCATCCA GCCACAGTGAACTCTTAAGGGGGTGATTAACACAAGTGTAAATCCTCAGTGAGCCACCGGTTGTCCTTAT GCTGCTGCCTTCCCTCTTTGGGACAGCCTGCTTATTGATTGCTGAAGCTAATCCAACTTTGGTTTTAACA GCGTGTCGGGATCTGTCAGAATCTCCAGTTGTTTCTTGGCTCGTGCACATAAACATTTTATGGTTTGAAT GTCCTAGGCTTCCTAAGTGTTTAGCATCATTTTTCTGTCATGTCTCAAAACGAACGTGTGGAGAAGAGCT AACCACTCGTAGAGGTGATACAAGAGGACTTAGAGTGGCCTTGGGTGTGTGTCACAAAGGCATTTTCATT TCGATATTGTCAGTATTGTCTCCCGTGTTGTTTAGTAGATCTCATTGTATTAATTTTTTTGAAATTATCT TCTATGATGTCTATCCATGGTTACTATACATGTTGTTCACTAAGGCTGAAAAAAAAATGGGGATATAAGT GTTCCAGTTCTCATAACTAATCATGACTATATATGACACAGTCCTCGGTGCTTCCTAGCCAGTGGTTGCT ATAATAAGCCAATGCAGGGTAAGTACATAAGGATTGTTGCCAACGACTTTGTGTTTTGAAAAGAGCTGAT ATGAAATACCCATTGTGTCCTCGCATACCTAAACACCAGTGCGGATAAATCCTGGAGGGTTGTCTCATTT TGCTCTTCCTGAAAGATGTCAAAAATAAATTATTTTAAGATGCCATGGAGTAAACATCAGCCTGCATATA TCTCCATCGTCTGCCAAATGCTTTTCTCAGTTAAAAGATATTTTCTCATAGAAAACTGAGCGTTTTCTTT CCTCACCTCCTATTAGCTATTTCAGTGATCATTTTATAAGTTAAAAGCTATAAATGAAACAAAGAAAAAA ATTTTTTAACATTTTTGCCTTGTACTCAAACTTTACTACTAAAAATTAAATTCCCAGAATACCAGTCTAT TAGCAACCTCTTTGTAGAGAAATTAACTATTCTGTACCCAACATATTTATTTATTTATTATCCAGCCATA GCAAAATAACAAAACAAGACAAAAAAGATTTGCTAAAACAGTTATTTGCTTTTTCTCCTTCCCTTCTATA CTCCATGTATATATATATATGTATATGGTCAGTGATTCCATTAAAGGGAAGCTGGTCATGAAAAAAATTC TATGTTTTCTCCCTATATTATATATCATGTCATGTATCGAATACAGTGGATAAAGAACTTGAGGTAGTGT TCCAATGACATTAGTTTCTATGAGAGAACAGATGTAGTGAGAGTTTTAATGGGGTTTATGAGAAGGCCCT CTTTAAAGTGTATATTACTCTGTAAACATTTCACTCAAGGGCCAGAAGTTAAAGTATAACTAAATCGCTG TGTTGTCAGAATAATATTTAACATTAACAAGAACAAACCCATGGTCAAACCAAAAAATAGTGGGTTGAAG TATACATTTCATTTTCTAATGCATTGGCAATTCGGATGACAAAGAAATTTAATATAAAGCTGTAGAGATA AATTCAGTGTCTAACCTTTTTTTTTAATTTATTTAAATAGTTGTGGCATTCTAGTCTTTACATTTTATGT CTGTCTTATTACTGTTCCTTCTTTCTAACACTTGTTTTCTGTAGGTTCACTGAATGCACAATAGTCACAT TTCACATGACTCAACTCCCAAGTGGTGTTAATCATTGGGCCTGTGTCATAAAATGCATGGATCTTTAATA CCTACATGTCGCTGACACTTTTCACTACAGGGCTGGACTTAGTAACTGACCAACTCGGGGGGGGGAGGGG GCGCTGGGGTATTAGAACATGATCAAAATGTCTCTGCTCAGGGATTTATGGTGGATCATTGCAGACAGTG CTAAAAATGTAGAGCACAAGACAAGTTTACTAAATTAAAATTTTATTTTTTGAAAAACTGTTATTTGTAT AAATTATCAAGATTTGTAGGCTTTCCTTTTGTAGAAATAATTGTTTGATGTGCCAGAGAATTTCAATTTT GTTTTGAACAATAAAGCATTGATAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6852756 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6852756 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12510 same embryo
	EMAGE:12508 same embryo
	EMAGE:12507 same embryo
	EurExpress:euxassay_011308 same experiment
	MGI:4828833 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source