EMAGE mouse gene expression spatial database

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EMAGE:12522

Sgsm3 small G protein signaling modulator 3 ( MGI:1916329)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011301. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011301_01	euxassay_011301_02	euxassay_011301_03	euxassay_011301_04

euxassay_011301_05	euxassay_011301_06	euxassay_011301_07	euxassay_011301_08	euxassay_011301_09

euxassay_011301_10	euxassay_011301_11	euxassay_011301_12	euxassay_011301_13	euxassay_011301_14

euxassay_011301_15	euxassay_011301_16	euxassay_011301_17	euxassay_011301_18	euxassay_011301_19

euxassay_011301_20	euxassay_011301_21	euxassay_011301_22	euxassay_011301_23	euxassay_011301_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12522_wholemount_strong.wlz

12522_wholemount_moderate.wlz

12522_wholemount_weak.wlz

12522_wholemount_possible.wlz

12522_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12522_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 08 09 10 15 16 17 18

brain

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 weak expression: see section 02 03 04 05 06 07 08

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 19 20 21

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 19 weak expression: see section 07

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 08 17 18 19 20 21 22 weak expression: see section 07

vagus x ganglion

moderate

regional

moderate expression: see section 18

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 05 06 08 09 16 17 18 19 weak expression: see section 07

spinal cord

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17 18

thoracic ganglion

moderate

regional

moderate expression: see section 13 14

dorsal root ganglion

moderate

regional

moderate expression: see section 10 11 12 16 17 18 19

ear

moderate

regional

moderate expression: see section 04 21 22 23 24

left lung

moderate

regional

moderate expression: see section 08 09 10 11 12

right lung

moderate

regional

moderate expression: see section 13 15 16 17 18 19 20 weak expression: see section 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T975
Entity Detected:	Sgsm3, small G protein signaling modulator 3 ( MGI:1916329)
Sequence:	sense strand is shown >T975 TCCTCGAGNCTGTTGGCCTACTGGAGCAGTGCGCGCCGACTAGGAGGCAGCTGCTAGCCTGACCTGCCGA GGTGCTGAGGTGGCCGCGAGACAGGGCTGATGAAGCCTCAGGACAGTCAGAGAAGCCAATGATGACCGCT TGTGCCTAGACCTATGAAGTCCTGCCGTCGTCCACAGCACATGATGTCAGGAAACCACACGCCTTCTGCC AGTGGCCCTTTCTCTGCCCTGACTCCAAGCATATGGCCCCAGGAGATCCTGGCAAAATACTCACAGAAAG AAGAATCCTCAGAGCAGCCAGAGCTCTGCTATGATGAGTTTGGTTTCCGTGTGGACAAGGAAGGTTCTGA GCCTGGATGCAGCCAGATGACAGGCTCACCCCTGGTGGAGGACCCCCCGCAGAGGCTACGTTGGCAGGCT CACCTGGAGTTCACCCATAACCATGATGTTGGGGATCTCACCTGGGACAAGATTGCAGTCTCCCTCCCCC GCTCTGAGAAACTCCGTTCCCTAGTGCTGGCTGGCATCCCACATGGCATGAGGCCAC
Notes:	The probe template was PCR amplified from IMAGE:1971820 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1971820 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12523 same embryo
	EurExpress:euxassay_011301 same experiment
	MGI:4828030 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source