EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:12591

Magi3 membrane associated guanylate kinase, WW and PDZ domain containing 3 ( MGI:1923484)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011395. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011395_01	euxassay_011395_02	euxassay_011395_03	euxassay_011395_04

euxassay_011395_05	euxassay_011395_06	euxassay_011395_07	euxassay_011395_08	euxassay_011395_09

euxassay_011395_10	euxassay_011395_11	euxassay_011395_12	euxassay_011395_13	euxassay_011395_14

euxassay_011395_15	euxassay_011395_16	euxassay_011395_17	euxassay_011395_18	euxassay_011395_19

euxassay_011395_20	euxassay_011395_21	euxassay_011395_22	euxassay_011395_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12591_wholemount_strong.wlz

12591_wholemount_moderate.wlz

12591_wholemount_weak.wlz

12591_wholemount_possible.wlz

12591_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12591_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 10

fibula

moderate

regional

moderate expression: see section 02

tibia

moderate

regional

moderate expression: see section 01 02

femur

moderate

regional

moderate expression: see section 02 03

facial vii ganglion

weak

regional

weak expression: see section 22

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 08 09 21

trigeminal v ganglion

weak

regional

weak expression: see section 04 05 06 07 08 09 18 19 20 21 22 23

vagus x ganglion

weak

regional

weak expression: see section 20

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 07 08 09 10 11 12 14 15 16 17 18

vomeronasal organ

moderate

regional

moderate expression: see section 11 14

left lung

weak

regional

weak expression: see section 07 08 09 10 11 12 13 14 15 16

right lung

weak

regional

weak expression: see section 17 18 19 20 21 22 23

axial skeleton

moderate

regional

moderate expression: see section 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37462
Entity Detected:	Magi3, membrane associated guanylate kinase, WW and PDZ domain containing 3 ( MGI:1923484)
Sequence:	sense strand is shown >T37462 ATCGTTGTGGAGGACTGAAAGTTGGAGATCACATCTCTGCTGTGAATGGGCAGTCCATTGTCGACCTATC CCATGATAATATTGTTCAGCTCATCAAAGATGCTGGAGTCACCGTCACGCTGACAGTGGTTGCTGAAGAA GAGCATCATGGTCCACCATCAGGAACAAACTCAGCCAGGCAGAGCCCAGCACTACAGCACAGGCCCATGG GACAAGCACAAGCCAACCACATACCTGGGGACAGAATTGCCCTAGAAGGTGAAATTGGGAGAGATGTCTG CAGTTCTTACAGACATTCCTGGTCTGACCATAAGCACCTTGCACAGCCTGACACTGCAGTGATTTCAGTT GTGGGCAGTCGGCACAATCAGAGCCTTGGCTGTTACCCTGTGGAGCTGGAGAGAGGCCCTCGAGGCTTTG GGTTCAGCCTCCGCGGAGGGAAGGAGTACAACATGGGGCTGTTCATCCTGCGCCTGGCCGAGGACGGGCC CGCCATCAAAGACGGCAGGATTCACGTTGGTGACCAGATCGTTGAAATCAATGGGGAACCGACACAAGGC ATCACACACACTCGAGCAATTGAGCTCATTCAGGCTGGTGGGAATAAAGTCCTCCTCCTTCTGAGGCCAG GAACTGGCTTGATACCTGACCACGGTTTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 66730. Forward Primer - name:066730_F_cDNA_6530407C02Rik, sequence:ATCGTTGTGGAGGACTGAAAGT; Reverse Primer - name:066730_N_SP6_cDNA_6530407C02Rik, sequence:CAAACCGTGGTCAGGTATCAA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12593 same embryo
	EMAGE:12596 same embryo
	EMAGE:12595 same embryo
	EMAGE:12592 same embryo
	EMAGE:12594 same embryo
	EurExpress:euxassay_011395 same experiment
	MGI:4826064 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source