EMAGE mouse gene expression spatial database

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EMAGE:12592

Pacs2 phosphofurin acidic cluster sorting protein 2 ( MGI:1924399)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011397. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011397_01	euxassay_011397_02	euxassay_011397_03	euxassay_011397_04

euxassay_011397_05	euxassay_011397_06	euxassay_011397_07	euxassay_011397_08	euxassay_011397_09

euxassay_011397_10	euxassay_011397_11	euxassay_011397_12	euxassay_011397_13	euxassay_011397_14

euxassay_011397_15	euxassay_011397_16	euxassay_011397_17	euxassay_011397_18	euxassay_011397_19

euxassay_011397_20	euxassay_011397_21	euxassay_011397_22	euxassay_011397_23	euxassay_011397_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12592_wholemount_strong.wlz

12592_wholemount_moderate.wlz

12592_wholemount_weak.wlz

12592_wholemount_possible.wlz

12592_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12592_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper arm muscle

moderate

regional

moderate expression: see section 01 02 03 04 05 06

upper leg muscle

moderate

regional

moderate expression: see section 22 23 24

diaphragm

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

forearm rest of mesenchyme

moderate

regional

moderate expression: see section 01 02 03

lower leg rest of mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 24

upper leg rest of mesenchyme

moderate

regional

moderate expression: see section 05 06 07 08 09 10

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

facial vii ganglion

strong

regional

strong expression: see section 04 05 06 07 08 21 22 23

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 08 20 21

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 18 19 20 21 22 23

vagus x ganglion

strong

regional

strong expression: see section 09 19 20

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 07 08 09 moderate expression: see section 19 20

trigeminal v nerve

strong

regional

strong expression: see section 10

spinal cord

moderate

regional

moderate expression: see section 12 13 14 15 16 17 18 19 20

dorsal root ganglion

strong

regional

strong expression: see section 10 11 12 13 14 15 18 19 20 21

neural retina

strong

regional

strong expression: see section 01 02 23 24

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 06 07 08 09 10 11 12 14 15 16 17 18

vomeronasal organ

strong

regional

strong expression: see section 11 14

tongue muscle

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17

tail paraxial mesenchyme

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37463
Entity Detected:	Pacs2, phosphofurin acidic cluster sorting protein 2 ( MGI:1924399)
Sequence:	sense strand is shown >T37463 TCACAGACAGAGATAGGGAGCATCCACAGTGCCCGGAGCCACAGGGAGCCTCCAAGCCCGGCTGATGTAC CTGAGAAGACGCGATCCCTGGGAGGCAAGCAGCAGCTCAGCGACAGCGTCTCTGACACGGTGGCCCTTAG TGCAGCAGTCCCCCGGGAACCATCAGGGCAACCTGAGGACAGCCCAGAGGCTGAGACCTCCACCTTAGAT GTGTTCACCGAGAAGCTGCCTCCCAGTGGAAGAATCATCAAGACTGAGTCACTTGTCATCCCCTCCACCA GGTCAGAATCAAAGCCGGCCGGCCGCCGGGGCCGGAGCACATCCCTGAAGGAGCGACAGCCTGCTCGGCC ACAGAATGAACGGGCCAACAGCCTAGACAATGAGCGCTGTCCAGACACGAGGAGCCAGCTTCAGATTCCC AGGAAAACTGTGTATGACCAATTGAACCACATCCTCATCTCTGATGACCAACTCCCCGAGAATATCATTC TCGTCAACACCTCTGACTGGCAGGGACAGTTCCTCTCAGATGTCCTGCAGAAGCATACACTTCCTGTGGT CTGCACGTGCTCTGCTGCTGACGTGCAGGCTGCCTTCAGCACCATTGTCTCTCGGATACAGCGATACTGC AACTGCAATTCTCAGCCACCGACCCCTGTGAAGATCGCAGTGGCGGGAGCGCAGCATTACCTCAGCGCCA TCCTGCGGCTCTTCGTGGAGCAGCTGTCTCACAAGACACCTGACTGGCTCGGCTACATGCGCTTCCTCAT CATCCCACTGGGCTCCCACCCCGTGGCCAGGTACCTGGGCTCTGTGGACTACCGCTACAACAACTTCTTC CAGGATCTGGCCTGGAGAGACCTGTTCAACAAGCTGGAAGCCCAGAGCAGTGTGCAGGACACACCAGACA TCGTGTCAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 87756. Forward Primer - name:087756_F_cDNA_6720425G15Rik, sequence:TCACAGACAGAGATAGGGAGCA; Reverse Primer - name:087756_N_SP6_cDNA_6720425G15Rik, sequence:GTGACACGATGTCTGGTGTGT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12593 same embryo
	EMAGE:12596 same embryo
	EMAGE:12595 same embryo
	EMAGE:12591 same embryo
	EMAGE:12594 same embryo
	EurExpress:euxassay_011397 same experiment
	MGI:4827022 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source