EMAGE mouse gene expression spatial database

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EMAGE:12688

Sv2b synaptic vesicle glycoprotein 2 b ( MGI:1927338)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011464. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011464_01	euxassay_011464_02	euxassay_011464_03	euxassay_011464_04

euxassay_011464_05	euxassay_011464_06	euxassay_011464_07	euxassay_011464_08	euxassay_011464_09

euxassay_011464_10	euxassay_011464_11	euxassay_011464_12	euxassay_011464_13	euxassay_011464_14

euxassay_011464_15	euxassay_011464_16	euxassay_011464_17	euxassay_011464_18	euxassay_011464_19

euxassay_011464_20	euxassay_011464_21	euxassay_011464_22	euxassay_011464_23	euxassay_011464_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12688_wholemount_strong.wlz

12688_wholemount_moderate.wlz

12688_wholemount_weak.wlz

12688_wholemount_possible.wlz

12688_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12688_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 11 12 13 14 15

epidermis

strong

regional

strong expression: see section 02 03 04 23 24 moderate expression: see section 01 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19

cerebral cortex marginal layer

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

telencephalon mantle layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 21 22 23 24

olfactory cortex marginal layer

strong

regional

strong expression: see section 10 11 12 13 14 16 17 18 19 20

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19

metencephalon rest of alar plate mantle layer

strong

regional

strong expression: see section 03 04 07

metencephalon rest of alar plate marginal layer

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20 21 22

metencephalon rest of alar plate ventricular layer

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

pons mantle layer

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

midbrain mantle layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 07 18

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 08 20 21 22 23 moderate expression: see section 09 18 19

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 06 07 08 18 moderate expression: see section 19

spinal cord mantle layer

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16

dorsal root ganglion

strong

regional

strong expression: see section 07 08 09 10 11 12 16 17 18

neural retina

moderate

regional

moderate expression: see section 01 02 03 04 05 24

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 09 moderate expression: see section 10 11 12 13 15 16 17 18 19 20

vomeronasal organ

moderate

regional

moderate expression: see section 13 16

esophagus

moderate

regional

moderate expression: see section 12 weak expression: see section 13

stomach

moderate

regional

moderate expression: see section 02 03 04 05 06

hindgut

weak

regional

weak expression: see section 12

rectum

weak

regional

weak expression: see section 13 14

midgut

weak

regional

weak expression: see section 10 11 12 13 14 15

renal cortex

weak

regional

weak expression: see section 07 08 09 10 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9470
Entity Detected:	Sv2b, synaptic vesicle glycoprotein 2 b ( MGI:1927338)
Sequence:	sense strand is shown >T9470 AACAAATTGAAAAGGTGATTTTATTGAAAAAGTCTCATGAGCATGCTCACCATTTCAATGATATAATAGA TGATGGTTATTGGAACAGCCCAAATATTTACCACAGAGCAAGAGATATAGCTTGAGCGGCTACCAGACAA TCCCAGAACCAAGGAATGGATGACTACAGGTATCGGGACAACTATGAGGGCTATGCCCCTAGTGATGGCT ACTACCGTAGCAATGAGCAGAATCAGGAAGAAGATGCACAGAGCGATGTTACAGAAGGCCATGACGAGGA AGATGAGATCTATGAAGGCGAGTACCAAGGCATCCCTCATCCAGATGATGTCAAGTCTAAGCAGACCAAG ATGGCGCCCTCCAGAGCAGATGGTCTTGGGGGCCAGGCAGACTTGATGGCTGAGAGGATGGAAGATGAGG AGGAGCTGGCTCACCAATATGAGACCATCATTGATGAGTGTGGCCATGGGCGCTTCCAGTGGACCCTCTT CTTCGTCTTGGGTTTGGCCTTGATGGCTGATGGAGTGGAAATATTTGTAGTGAGCTTTGCTCTGCCAAGT GCAGAGAAAGACATGTGTCTGTCCAGTTCCAAGAAAGGAATGCTTGGGCTGATAGTCTACCTAGGAATGA TGGCAGGGGCCTTCATCTTGGGCGGCCTGGCTGATAAACTGGGAAGGAAGAAGGTCCTCAGCATGTCACT GGCCATCAATGCCTCCTTTGCTTCACTCTCCTCCTTCGTGCAGGGATATGGAGCCTTCCTCTTCTGCAGA CTCATCTCAGGCATAGGGATTGGGGGCTCCCTGCCAATTGTTTTTGCCTACTTTTCTGAGTTCTTATCAC GAGAGAAACGCGGTGAGCATCTTAGCTGGCTGGGCATCTTCTGGATGACTGGAGGCATCTATGCATCTGC CATGGCCTGGAGCATCATTCCACACTATGGCTGGGGCTTCAGCATGGGGACTAATTACCACTTCCACAGC TGGAGAGTGTTTGTCATCGTCTGTGCTCTGCCCGCCACTGTGTCCATGGTGGCTCTGAAGTTCATGCCAG AAAGCCCCAGGTTCCTGCTGGAGATGGGCAAACATGATGAAGCCTGGATGATTCTCAAACAAGTCCATGA TACAAACATGAGAGCTAAGGGGACCCCGGAAAAGGTGTTCACGGTTTCCCACATCAAAACTCCCAAGCAA ATGGATGAATTCATTGAGATACAGAGCTCAACCGGAACTTGGTACCAGCGCTGGCTGGTCAGGTTCATGA CCATTTTCAAACAGGTCTGGGATAACGCCTTGTACTGTGTGATGGGGCCCTACAGAATGAACACCCTGAT TCTGGCTGTGGTCTGGTTCACCATGGCCTTAAGTTACTACGGCCTGACGGTGTGGTTCCCCGACATGATC CGCTATTTCCAGGATGAAGAATATAAGTCTAAAATGAAGGTGTTTTTTGGTGAGCACGTGCATGGCGCCA CGATCAACTTTACCATGGAAAACCAGATCCACCAACATGGGAAGCTTGTGAACGATAAGTTCATAAAGAT GTATTTTAAACATGTCCTCTTTGAGGACACATTCTTTGACAAATGCTATTTTGAAGATGTGACATCCACA GATACGTATTTCAAGAACTGCACCATCGAATCGACCACCTTCTACAACACAGACCTCTACAAACACAAGT TCATCAACTGTCGGTTTATCAATTCCACCTTTCTGGAGCAGAAGGAGGGCTGCCACATGGACTTTGAAGA GGACAATGATTTTCTGATTTACCTCGTCAGCTTCCTCGGCAGCCTCTCTGTCTTGCCTGGGAACATAATT TCTGCCCTGCTCATGGACAGAATTGGAAGACTCAAGATGATTGGCGGCTCCATGCTCATCTCTGCAGTCT GCTGCTTCTTCCTGTTTTTTGGCAACAGCGAGTCTGCGATGATCGGCTGGCAATGCCTGTTCTGTGGGAC CAGCATTGCAGCCTGGAATGCTCTGGATGTGATCACAGTTGAGCTGTATCCCACCAACCAGAGGGCCACG GCCTTCGGCATCCTCAACGGACTGTGCAAGTTTGGGGCCATCCTGGGAAACACTATCTTTGCTTCTTTTG TTGGGATAACCAAAGTGGTCCCCATCCTTCTGGCTGCTGCTTCTCTGGTTGGAGGTGGCCTGATTGCCCT TCGATTGCCAGAGACTCGAGAGCAAGTCCTGATGTGACCAACCCACCGGGAAAAGACAGATGGGAAGATC TTTTTCAGGACACTCCAGCACAGCCACGCCTCCTGCCTCTCACTGTCTACAGGATACCGCGGACAGCACG GAAGGAGAAATGGACTTTGTGATCCCTAGTTTAGGACCCACTGCAGTTAATACATTTTTAACTCAGGTGA TTGACTAGGGGTGCCCAGAACCATCCTTGGGATCCCAGAGCTGTGTGCTTAGCTTCAGGTCTCTTCATCC CAGGCAGGGAGAGGACACTCCAGTAAACACACACAGTAAGCAAGGAGTATATGCTCCTCTAAATGAGGTG CAAAAAACTATTTGCATTTCGGACTGAAGTTGAGGACTAGAACCACTTAAGCTCCTCCATGCAGCTTCTG GGAGCCCAGTGACCTAGCAGATTGTCATAGTGGGGAGTGGGGTCAACACATACTGGGCTCAGAGAGCAGA CTACGTATTTGCATTTGAAAGTATCACCTATCATATTTTCCTTTTGAAAACTGGCACAGTAGCCTTGAGG ATACTCTGATATAGAAAAACCCAACCTTTAAGCAAAATGTATATAGACGGACTTTTCTTCCCAAGCATCC TGAACTTTGCATAACGAAGAGGTGATACACTAAGAACTATATTTATTATGGTTTTGCTCTTGTCAGAGCA ACGTGAGGATGGGTATTATGTAAACTAAGTTTGTATATAAGAGTTTTGAGGTTTTATTCCCACAAATGGT GGCTGCTAAGGTTGCCAACATTTTAGCCATATTTGGGGAGGACTCTCCATTCAAGGTCGCAGGAAACTTG GTGCGAATGGGTAGCATCTTGCTTGCTGTCCGGAGGATGTACTTGGCAAGCCACGTGCCATTTCCTTTAC CTGGGAACTTTTCTTCAAATTGGTAGGGCAGGTACTTCTCTCTCCTGTGTTGCTCATTTGTGAAACTCAG GACCATAGGTTGTTTGATGCTGTTTACCCAGAGAGAACAGGAAGAAATGAAATGAAAATGTTTTTATTTT TATTATTGGCTGCTTGCACTCCTTTTCTTTAGAGAGAACCCCCTCACAGATAACTGACTAAGGATGGGGA AAGGTAACCACAGTTCTTCAAAATGTGAGTTCTTCCTGTTTGATTTCAAAGAAAAACACAATGTACATTT ATTATAGTGTTTAGGACCTATGAGAAATTTATGCTCTGTGTGTTGGCTCGGGGCGTTCGAAACAGGCCAT TAGCATGCACATTAGGAAATAAAAGGCATGAGATACATTCGTTTCTTCATTGCTCCAAGGAAGCTTTTTC TGAGATCCAGGTTGGAACTTGAATGTGGAAAGGGGAGAGGGGGTCAGGGCTGCTCTGGCAGGTGCCAGCA GTCTGGAGGCTAGGCTGAGGGAGTGGCTTTTGTTCATCAGTACCCTCTGCAATGGTAGAGACAGAGCTAG GAGTTCCTGCCACCTGAGCTGCTCTTACCTGGGCCAGCAGAACGACTGACTGACTGACTGTAAGAACTAG TTTGCTCACGTTATTTGCAGCACACACACAAGCTGTGATGTGCTAATCTCTACCTATTAGAAATCTTCCC AAATCTAAACCAAACTAATGACTACATTGAGTGTTTTCTCAGTTCTGTGGAAATACTTAGATGCCAGGCC AGTTCAAGCCATCTTTGCCTAGTCCTCTGGAATATAAGTTGGCAGTGGGAAAGAGTGAAATCTTTGAGAA CTTCCCATTTTCAAGTGTGGCTAACATGTTTACTCTGCTTCTCTGGTCCCCACCTCTTTATTTACACCAC CATTACTTTCTGTCCAGGGAAGGAAGATGCATCAGGTCTGATGTGATAACCCCCATTACTCCAAATATAA ATATATATAAATATACAGAATGTATTAATGCTTCTCTCGGTGTTTTATAGGAATTAACTGATAACGCTCT GTTGATAACGAGAAACTTTCTGTAATATAGCTGTGATGTCCTTTTCCATTTTCTTGTGATATGGAATGCA TTGCTTAGGATCATGAGAGAATTGTATAAATCTGTGGTTATGTATCCCTCATAACAGTGTTCTTTAATGG GATAATAGACCTCAGTGCTCAGCTACCACTCAAGAAATGTACGAGACAGTTAGAATCCACATTTACAGTC AGATATATCTTGTAAACTTTAAATCTGACTAATTTTTAAAGCACAGGAGTGGCTGCAGTTATTTTAGGCT AACTGTTCTTGGTTTCTAAGCTCTGTCTTCTACATTACATTTATGGCTCCTTAACCTGTTTGCCTAACCA ACCAAGGGCAATTCCCGTCCATCCATCACACGGGATGTGAGAAGGATGCAGCCATGGTCTGCAGCCTCTC TCATGAAGGGTTATCTGGCCATGGTACTTGGCTGCTTCATAACTTGCTGTCACTCCTGATGAGATAAGTG TGTCTTTAAAAATAGTCCCCGTGTCAGGTCACTGGGGTGTAATGTACAACATCCTTAGCCATCCATTTTT TCTTTTCTTTTTTTTTGCCCTGAGAGACTCCCAATGGCATCTATAGAGGACTAAAGAAGAGAGCCGTTCC CTCACTGAGTCAGACTGTTCACAGTTCATTATGTATTTTATGATGGCTTGTTTGGGAGCCGATACTTTCA GCAGTCTCAACTGTGTTGTGGGATCTCCTGATGCTGGTTTCTGAGCTTTTGTTTTATTAAAACTGATTAG TGAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30356990 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30356990 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12689 same embryo
	EMAGE:12690 same embryo
	EurExpress:euxassay_011464 same experiment
	MGI:4828546 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source