EMAGE mouse gene expression spatial database

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EMAGE:12766

Tmem66 transmembrane protein 66 ( MGI:1915137)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011510. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011510_01	euxassay_011510_02	euxassay_011510_03	euxassay_011510_04

euxassay_011510_05	euxassay_011510_06	euxassay_011510_07	euxassay_011510_08	euxassay_011510_09

euxassay_011510_10	euxassay_011510_11	euxassay_011510_12	euxassay_011510_13	euxassay_011510_14

euxassay_011510_15	euxassay_011510_16	euxassay_011510_17	euxassay_011510_18	euxassay_011510_19

euxassay_011510_20	euxassay_011510_21	euxassay_011510_22	euxassay_011510_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12766_wholemount_strong.wlz

12766_wholemount_moderate.wlz

12766_wholemount_weak.wlz

12766_wholemount_possible.wlz

12766_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12766_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pituitary gland

strong

regional

strong expression: see section 11 12 13 14 15 16

medulla oblongata alar plate mantle layer

moderate

regional

moderate expression: see section 14 15

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 09 10 16 17

pons mantle layer

moderate

regional

moderate expression: see section 08 09 16

vagus x ganglion

strong

regional

strong expression: see section 09 10 19 20

ventral grey horn

moderate

regional

moderate expression: see section 12 13 16 17 18

dorsal root ganglion

moderate

regional

moderate expression: see section 10 11 12 13 17 18 19 20

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 08 09 10 11 12 14 15 16 17 18 19

vomeronasal organ

strong

regional

strong expression: see section 12 15

lower jaw incisor

strong

regional

strong expression: see section 12 13 16 17

lower jaw molar

strong

regional

strong expression: see section 08 09 19 20

upper jaw incisor

strong

regional

strong expression: see section 11 12 16 17

upper jaw molar

strong

regional

strong expression: see section 08 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30966
Entity Detected:	Tmem66, transmembrane protein 66 ( MGI:1915137)
Sequence:	sense strand is shown >T30966 GCACCAGGGCTTCTCTGATTATTATCACAAGCTGTACTCCTCAGATTCCTGTGGCTTTATTACCATTGCA GTACTGTTTGTTCTCGCCTTTGCGGTTTACAAGCTGTTCCTCAGCGATGGCCAGGGGTCGCCTCCGCCGT ATTCTGAGCACCCGCCATACTCAGAGCACTCTCAGAGGTTTGCCAGTGCCGCAGGGGCGCCTCCTCCGGG CTTTAAGTCGGAGTTCACAGGACCACAGAATACTGGCTATGGTGCAAGCTCTGGCTTCGGGAGTGCTTTT GGAGGCCAAGGCTATGGCAGTTCAGGGCCGGGGTTCTGGTCTGGCCTGGGAGCTGGAGGACTGCTTGGGT ATTTGTTTGGCAGCAACAGAGCGGCGACGCCTTTCTCAGACTCGTGGTACCATCCAGCCTACCCTCCTTC CCACTCTGGGGCCTGGAACAGTCGGGCCTACTCACCCCTGGGTGGAGGCGCAGGGAGCTATTGTGCATCC TCTAATGCAGACTCGAGAACCAGAACAGCATCAGGATATGGTGGCACCAGAAGACGGTAAAATAGGAAAT TGAAGGCAAACACTGGATGCAAAGTTTCTGATTTGTCATCACCATCTCTTTAACACCTGGCTAATGGGAA TAACCAAAAGTTGTGTGGTGTTTGGTCCTTGTGTAGCTTTTCGTGTCCCGTTGTTTGTGGCCAAGAGTTG ATATGTGACAAAATGCTATGGTTTGTATGATAATGTAACATGCAGTTCAAAGCGATGGCCACTGTGGAAT GCTGACATTGTGCTTGTGTCTCAAGCCTTTCATACCAGAGGGGGC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4222385), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 19291. Forward Primer - name:019291_F_IRAV28-31_N01_1810045K07Rik, sequence:GCACCAGGGCTTCTCTGA; Reverse Primer - name:019291_R_SP6_IRAV28-31_N01_1810045K07Rik, sequence:TGCCCCCTCTGGTATGAA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12764 same embryo
	EMAGE:12761 same embryo
	EMAGE:12765 same embryo
	EMAGE:12762 same embryo
	EMAGE:12763 same embryo
	EurExpress:euxassay_011510 same experiment
	MGI:4828814 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source