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EMAGE:1277

Vcam1 vascular cell adhesion molecule 1 ( MGI:98926)
TS20 (11.5 dpc)
immunohistochemistry

Data Images
EMAGE:1277
Figure 3E of Kwee et al., 1995 [PMID:7539357] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:1277EMAGE:1277Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
1277_voxel_strong_3D_1.wlz
1277_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:1277_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
interventricular septum cardiac muscle
strong strong
regionalExpression is in the compacted layer of the myocardium and in the intraventricular septum.
left ventricle cardiac muscle
strong strong
regionalExpression is in the compacted layer of the myocardium and in the intraventricular septum.
right ventricle cardiac muscle
detected detected
regionalExpression is in the compacted layer of the myocardium and in the intraventricular septum.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:MGI:1334372
Entity Detected:Vcam1, vascular cell adhesion molecule 1 ( MGI:98926)
Notes:The anti-VCAM1 antibody used in this study by Kwee et al., 1995 [PMID:7539357] is described as follows, "anti-VCAM-1, 429 (Pharmingen No. 5741)." As of 17/12/04 there are three Pharmingen catalogue numbers for 429: 553329, 550547 and 553332. Source of antigen below is taken from these.
Antibody Type:monoclonal
Raised In:rat
Supplier:Pharmingen
Catalogue Number:5741 (expired)
Specimen
Organism:mouse
Strain:CD-1
Age:11.5 dpc
Theiler Stage:TS20
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:methanol
Embedding:cryosection
Secondary Antibody:goat anti-rat IgG
Labelled with:FITC
Visualisation method:fluorescence
General Information
Authors:Kwee et al., 1995 [PMID:7539357] Indexed by GXD, Spatially Mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:7539357] Kwee L, Baldwin HS, Shen HM, Stewart CL, Buck C, Buck CA, Labow MA 1995 Defective development of the embryonic and extraembryonic circulatory systems in vascular cell adhesion molecule (VCAM-1) deficient mice. Development (121):489-503
Links:MGI:1334379 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI