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EMAGE:1279

Vcam1 vascular cell adhesion molecule 1 ( MGI:98926)
TS12 (8.75 dpc)
immunohistochemistry

Data Images
EMAGE:1279
Figure 2B of Kwee et al., 1995 [PMID:7539357] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: h - heart; a - allantois.
Expression Pattern Description
Spatial Annotation:
EMAGE:1279Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
1279_wholemount_strong_3D_1.wlz
1279_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:1279_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
allantois
detected detected
regionalExpression is at the distal end of the developing allantois and in the myocardial cells of the heart.
heart
detected detected
regionalExpression is at the distal end of the developing allantois and in the myocardial cells of the heart.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:MGI:1334372
Entity Detected:Vcam1, vascular cell adhesion molecule 1 ( MGI:98926)
Notes:The anti-VCAM1 antibody used in this study by Kwee et al., 1995 [PMID:7539357] is described as follows, "anti-VCAM-1, 429 (Pharmingen No. 5741)." As of 17/12/04 there are three Pharmingen catalogue numbers for 429: 553329, 550547 and 553332. Source of antigen below is taken from these.
Antibody Type:monoclonal
Raised In:rat
Supplier:Pharmingen
Catalogue Number:5741 (expired)
Specimen
Organism:mouse
Strain:CD-1
Age:8.75 dpc
Theiler Stage:TS12
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Secondary Antibody:goat anti-rat IgG
Labelled with:horse radish peroxidase
Visualisation method:DAB
General Information
Authors:Kwee et al., 1995 [PMID:7539357] Indexed by GXD, Spatially Mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:7539357] Kwee L, Baldwin HS, Shen HM, Stewart CL, Buck C, Buck CA, Labow MA 1995 Defective development of the embryonic and extraembryonic circulatory systems in vascular cell adhesion molecule (VCAM-1) deficient mice. Development (121):489-503
Links:MGI:1334377 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI