EMAGE mouse gene expression spatial database

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EMAGE:12831

C1galt1 core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase, 1 ( MGI:2151071)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013628. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013628_01	euxassay_013628_02	euxassay_013628_03	euxassay_013628_04

euxassay_013628_05	euxassay_013628_06	euxassay_013628_07	euxassay_013628_08	euxassay_013628_09

euxassay_013628_10	euxassay_013628_11	euxassay_013628_12	euxassay_013628_13	euxassay_013628_14

euxassay_013628_15	euxassay_013628_16	euxassay_013628_17	euxassay_013628_18	euxassay_013628_19

euxassay_013628_20	euxassay_013628_21	euxassay_013628_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12831_wholemount_strong.wlz

12831_wholemount_moderate.wlz

12831_wholemount_weak.wlz

12831_wholemount_possible.wlz

12831_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12831_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vertebral axis musculature

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 16 17 18 19 20 21 22

thymus primordium

moderate

regional

moderate expression: see section 10 weak expression: see section 11 12 13 14

vibrissa

weak

regional

weak expression: see section 04 05 06 07 19 20 21 22

telencephalon ventricular layer

weak

regional

weak expression: see section 01 02 03 04 05 06 08 16 17 18 19 20

stomach

moderate

regional

moderate expression: see section 05 06 07 08 09 weak expression: see section 03 04

mandible

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 16 17 18 19 weak expression: see section 20 21

maxilla

moderate

regional

moderate expression: see section 05 06 07 08 16 17 18 19

liver lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

bladder

weak

regional

weak expression: see section 13 14

urethra of male

weak

regional

weak expression: see section 14 15

lung

weak

regional

weak expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

orbito-sphenoid

weak

regional

weak expression: see section 01 02 03 19 20 21 22

rectum lumen

moderate

regional

moderate expression: see section 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8134
Entity Detected:	C1galt1, core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase, 1 ( MGI:2151071)
Sequence:	sense strand is shown >T8134 TGGAGCTGGTCGGTTATGGTTGGAACTTTCCCTGCCCTTCGTTGACTGAGTGAACCAGCCAGAAATACAT TCCCAGGAAATGGCCTCTAAATCTTGGCTGAATTTTTTAGTCTTCCTCTGTGGATCAGCAATAGGGTTTT TTTTATGTTCTCAACTCTTGAGTATTTTGTTGCGAGAAGAGGCTGCCATTCAGCCTAACATGCTTCACAA TGACCCTCATGCAAGGCATTCAGATGACAATGGACACAGTCACCTCAAAGGACAGATGAACTTCAATGCA GATTCCAGCCAACATAAAGATGAGAACATAGACGTTGCTGAGAACCTCTATCAGAAAGTTAAAATTCTTT GTTGGGTTATGACAAGTCCTCAAAATCTAGAGAAAAAGGCCAAACATGTCAAAGCTACGTGGGCCCAGCG TTGTAATAAAGTGTTATTTATGAGTTCGGAAGAAAATCAGGACTTCCCTACTGTGGGATTGAAAACCAAA GAAGGCAGAGAGCAACTATATTGGAAAACAATTAAAGCTTTCCAGTATGTACATGACCATTATTTAGAAG ATGCTGACTGGTTTATGAAAGCAGATGACGACACATACGTCATTGTGGACAACCTGAGATGGCTTCTATC AAAGTATGACCCTGAACAACCCATTTACTTTGGGCGAAGATTTAAGCCCTATGTGAAGCAGGGATACATG AGCGGAGGAGCGGGCTATGTCCTAAGCAAGGAAGCCTTGAGAAGATTTGTTAATGCATTTAAAACAGAAA AGTGTACACATAGTTCCTCCATCGAAGACTTAGCTCTGGGAAGGTGCATGGAAATTATAAATGTAGAAGC TGGAGATTCCAGAGATACCATTGGGAAAGAAACCTTCCATCCATTTGTACCAGAACACCACTTAATCAAA GGTTATCTACCAAAAACATTTTGGTACTGGAATTACAACTATTATCCTCCCATAGAGGGTCCTGGATGCT GTTCTGATATTGCAGTTTCTTTTCACTATGTTGATGGGACAACTATGTATGAATTAGAATACCTCGTTTA TCGTCTTCGTCCATATGGTTGTTTATATAGATATCAACCTGCCTTACCTGAGAATATACTGAAAGAAATT AATCAAGTAAACAGAAAGGAAGATACAAAAATAAAATTAGGCAACCCCTGAAAGCAGAACATAAGTGGTC TACATTGCACTGAAGGACTCTTGCCTTTCTACGGAACCCTGAAATCCCAGTGAGGAACTCACCTGAAGTG AACATTCCATATAGAAATCTTTCAAATGGATGACTATAAACTGAAGCATTTAAAGAGCTGTGAAGTTTGC TAAAACGTGTCTTGATACAGTAATATATAAATATAAATAAATATATATGAAGAATTTGTGTTTTTAAATG GTGACAGAAGAGCTAGAAAGACTCTGTTTCTGACTGTTCCCAACAGTGTATGTTGTTATCACTACAGTTG GAGTGGTTAGTTTATAATCACCTTCTGCACCACACCAGTTAGAACACAATGCTGAACAGATCTGCCTTAA AGAAAGAAAGAAAGAACTGTTGCTCAGTGTTGTTTCTCAGCTGATATGTGTATGCTGCAAACCAGCCTTC CAGTTGCCGAATGAAAACTTTCCTGTGATTATATTATTTATAATTGGCAGTGCTTCCTCAAGAAGAATAA GAAGGGCACTTACCCCAAAGTGCAGTAATTAACCACATTTCCAATTATTCCGAGCCTTGACTCCATTAGA TACTTAGTTCTAAAGATAATCAACCCGATACCAAGTCCCCATTGTCCCCTTTCCAAATGTTTGACCCTGA ATACCATTTTTCACTATTAATGCTAATTTACTCAAAGCATTGGCAGCATTCTGTAAATCATGCATTTTCA AATATGTTGTTCCCCGATTCACCCATTGTTAATTGTAGGTAACAATTAAATTTAATGCTGTTTGGACAAG CAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30077842 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30077842 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12832 same embryo
	EurExpress:euxassay_013628 same experiment
	MGI:4823533 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source