EMAGE mouse gene expression spatial database

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EMAGE:12839

Brap BRCA1 associated protein ( MGI:1919649)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011623. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011623_01	euxassay_011623_02	euxassay_011623_03	euxassay_011623_04

euxassay_011623_05	euxassay_011623_06	euxassay_011623_07	euxassay_011623_08	euxassay_011623_09

euxassay_011623_10	euxassay_011623_11	euxassay_011623_12	euxassay_011623_13	euxassay_011623_14

euxassay_011623_15	euxassay_011623_16	euxassay_011623_17	euxassay_011623_18	euxassay_011623_19

euxassay_011623_20	euxassay_011623_21	euxassay_011623_22	euxassay_011623_23	euxassay_011623_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12839_wholemount_strong.wlz

12839_wholemount_moderate.wlz

12839_wholemount_weak.wlz

12839_wholemount_possible.wlz

12839_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12839_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

weak

regional

weak expression: see section 07 08 09 21 22 23

hypothalamus mantle layer

weak

regional

weak expression: see section 10 11 12 15

diencephalon lateral wall mantle layer

weak

regional

weak expression: see section 10 11 12 15

telencephalon mantle layer

weak

regional

weak expression: see section 09 10 11 12 13 14 15

midbrain mantle layer

weak

regional

weak expression: see section 09 10 16 17 18

facial vii ganglion

weak

regional

weak expression: see section 05 06 21 22

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 07 08 19

trigeminal v ganglion

weak

regional

weak expression: see section 04 05 06 07 08 09 10 18 19 20 21 22 23

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 07 08 19

ventral grey horn

weak

regional

weak expression: see section 11 12 14 15 16 18

dorsal root ganglion

weak

regional

weak expression: see section 08 09 10 11 13 14 17 18 19 20

neural retina

weak

regional

weak expression: see section 01 02 03 04 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31677
Entity Detected:	Brap, BRCA1 associated protein ( MGI:1919649)
Sequence:	sense strand is shown >T31677 GAGCTGCAGGAGGAGCAGGAGCTGAACAAGTGTCTGCGCGCCAACCAGCTGGTGCTGCAGAACCAGCTCA AGGAGGAGGAGAAGCTGCTGAAGGAGACCTGTGCCCAGAAAGACCTGCAGATCACCGAGATCCAGGAGCA GCTGCGCGATGTCATGTTCTACCTGGAGACACAGCAGCAGATCAGCCACCTGCCTGCGGAGACGAGGCAG GAGATCCAGGAAGGCCAGATCAACATCGCCATGGCCTCAGCGCCCAACCCACCCTCTTCCGGGGCCGGTG GGAAGCTGCAGTCCAGAAAGGGCCGCAGCAAGAGGGGCAAGTGACCATCAGAGACTTGTCCCTGAGACTT CCTGGGCACCACAGGGTCTGCTGGGACCTTTGCTGAGCGGAAGTGTGGGCCCTAATAAGTACGTCTGAGG ACGAACTGTGGTCATGTGGCTCCTTCATTTGTTGAGTGTAGTGTGAGGAGACTAAAAGAGGGGGAAGGGT TGGCAGCACTTCCCAAGGGTGAGTCACCTCATGGCCATTCTGCCTTCCAGCTGGGTAGCCCCCAGGCTTG TGCGGTTTTCCATTGTATTTATTCAGTTGGCGCACTTGCAGTTGGGTTCCAGGGTAAATCTATGACATGA CGTAACCTTCCTAGCACTAAAGGAATTCCAGCATTAACTAGCACTAAAGCACAGTGCCTCCCAGAGGCCC AGCTGCCTTCAGGAGAGGAGCCCACTGCCCACTGCAGAAGGTCTTTCCTGCCATCCCTCCCTCCCTTCTC CTGCAGAGTAGAAGAGAGAGCTGCTTGCATGTCATAGAGAAAGGGCTAGGTTGTAAACCAAGCACAGGCT TGTCTTCAGCAGCCTGGCTTGGGGCAAATGGAGTATACCCAGTTTAGGGAAGACAGTGACAGCTCTCTTT ATTTCTGATGACTCCATCCGAAATGTGC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3599718), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 33389. Forward Primer - name:033389_F_IRAV81-84_C14_Brap, sequence:GAGCTGCAGGAGGAGCAG; Reverse Primer - name:033389_R_SP6_IRAV81-84_C14_Brap, sequence:GGCACATTTCGGATGGAG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12840 same embryo
	EMAGE:12842 same embryo
	EMAGE:12838 same embryo
	EMAGE:12843 same embryo
	EMAGE:12841 same embryo
	EurExpress:euxassay_011623 same experiment
	MGI:4823497 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source