EMAGE mouse gene expression spatial database

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EMAGE:12868

Trrap transformation/transcription domain-associated protein ( MGI:2153272)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013672. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013672_01	euxassay_013672_02	euxassay_013672_03	euxassay_013672_04

euxassay_013672_05	euxassay_013672_06	euxassay_013672_07	euxassay_013672_08	euxassay_013672_09

euxassay_013672_10	euxassay_013672_11	euxassay_013672_12	euxassay_013672_13	euxassay_013672_14

euxassay_013672_15	euxassay_013672_16	euxassay_013672_17	euxassay_013672_18	euxassay_013672_19

euxassay_013672_20	euxassay_013672_21	euxassay_013672_22	euxassay_013672_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12868_wholemount_strong.wlz

12868_wholemount_moderate.wlz

12868_wholemount_weak.wlz

12868_wholemount_possible.wlz

12868_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12868_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

weak

regional

weak expression: see section 08 09 10 11 18 19 20 21 22

vibrissa

weak

regional

weak expression: see section 07 08 09 10 20 21 22 23

facial vii ganglion

weak

regional

weak expression: see section 05 06 21 22

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 07 08 09 19 20

trigeminal v ganglion

weak

regional

weak expression: see section 04 05 06 07 08 09 10 18 19 20 21 22

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 07 08

dorsal root ganglion

weak

regional

weak expression: see section 08 09 10 11 12 16 17 18

lower jaw incisor

weak

regional

weak expression: see section 12 13 16 17 18

lower jaw molar

weak

regional

weak expression: see section 08 09 19 20

upper jaw incisor

weak

regional

weak expression: see section 12 13 16 17

upper jaw molar

weak

regional

weak expression: see section 08 09 19 20

testis

strong

regional

strong expression: see section 06 07 20 21 22 moderate expression: see section 05 08

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31635
Entity Detected:	Trrap, transformation/transcription domain-associated protein ( MGI:2153272)
Sequence:	sense strand is shown >T31635 TCTCTGCCTTGTTCCCCACTTTATTCAGTTACCTTCCCAGTAGTTTGTGTGTGGAAGAGCATGAGATTAG TCCTGGAGGGAGCCCCTGCACACAGTAGGCCAGGCCGTTGGCGATGGCAGAGCTGAACTCAGCAGCTTCC GAGATGCCTTTTAACTTGAGGAGCCCTGAACTGGGACTCCCTGTGTACTCCTGTCTCAGTGTGAAGTGCA GACTGAGAACGTCCCCTCCATCCCCCAACTTCCAGAAACTACACCTCAGAGTGGCTGCTCACTGTCATAC CGGGTCTAGGGAATCCACAGTGTTAGAGAAGCAGGAAGCAGGATCAGTTCGACAGGCGTGCATCTCTGAG CACAGATCTCACTCTGTCACTGGTGTGGACATCTCGCCAAGTCTGATGATGAGGCTCAGCGAGCCCAGGG CCTGCAGGGGAAGCTGCTCCAGGCCTCATCAGTCAGATCCGATGCAGCTGTGCAGAACGAAGCTGTGAGG CCAGCGGGCCAGCCTTTCTCTTTTTACTGCCGGCTTGTTGGATATCTTTTAGAGATTTACTATCATTGTA CCAGAAAGAAGAAAAAAAAAAGACAAAATCATTTTGTTTTTCCTTCCTAATTTAAAAAGAAAGCAGTGAA AGTTTGTCCC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:2655777), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 38398. Forward Primer - name:038398_F_IRAV08-11_F01_AI481500, sequence:TCTCTGCCTTGTTCCCCA; Reverse Primer - name:038398_R_SP6_IRAV08-11_F01_AI481500, sequence:GGGGACAAACTTTCACTGCT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12867 same embryo
	EMAGE:12866 same embryo
	EMAGE:12864 same embryo
	EMAGE:12865 same embryo
	EurExpress:euxassay_013672 same experiment
	MGI:4828945 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source