EMAGE mouse gene expression spatial database

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EMAGE:12907

Stx12 syntaxin 12 ( MGI:1931027)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011670. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011670_01	euxassay_011670_02	euxassay_011670_03	euxassay_011670_04

euxassay_011670_05	euxassay_011670_06	euxassay_011670_07	euxassay_011670_08	euxassay_011670_09

euxassay_011670_10	euxassay_011670_11	euxassay_011670_12	euxassay_011670_13	euxassay_011670_14

euxassay_011670_15	euxassay_011670_16	euxassay_011670_17	euxassay_011670_18	euxassay_011670_19

euxassay_011670_20	euxassay_011670_21	euxassay_011670_22	euxassay_011670_23	euxassay_011670_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12907_wholemount_strong.wlz

12907_wholemount_moderate.wlz

12907_wholemount_weak.wlz

12907_wholemount_possible.wlz

12907_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12907_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 08 09 15 16

pons mantle layer

weak

regional

weak expression: see section 06 17

facial vii ganglion

weak

regional

weak expression: see section 04 05 20 21 22

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 06 07 18 19

trigeminal v ganglion

weak

regional

weak expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22

vagus x ganglion

weak

regional

weak expression: see section 08

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 06 07 18 19

dorsal root ganglion

weak

regional

weak expression: see section 09 10 11 12 13 16 17 18

neural retina

weak

regional

weak expression: see section 01 02 03 23 24

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 09 10 11 12 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31632
Entity Detected:	Stx12, syntaxin 12 ( MGI:1931027)
Sequence:	sense strand is shown >T31632 TGCATGTGGAAAGAGCCACTGATCAGCTACAAAGAGCTGCTTACTATCAGAAAAAATCTCGCAAGAAGAT GTGTATCCTCGTGCTTGTCCTGTCAGTGATTGTTACAGTCTTGGTAGTTGTTATCTGGGTTGCTTCTAAA TGAAGTGCTTGCCTCAGAGCCTCCTCCTGCTGAGTTGTTTTCAAGGGCAAGTGCTTGAGAGAGTCTTGCC AGAACAGACTGACCACAGAAGAGAGTGTACCAGAGTGTCCTGTGATAATTTAGCTAGAATCTAACTACTA ACTAGTCCTTGGAATTAGTGACCATGGAGACAGTATTTATCAGTTTATGCATACATTCTATTCATTTGTC AAATAACGAATTACCTTATTCCTTTGTATTTTCCTTTCTCTTGTATTTTGATTGCCCTTATTGCAAGTGT TTATTGACAGTTCCATCGTGGATATTCTTTTTGACAGGTGACACTACATCATAAATTTGTCTTTTTGTAT ATACGCAAACTTTACCTTTTTATGAGTATCTGAGATAAATTTCCATCTGGAGCCAGCCTATTAGAGTCTG AGAAACTGTACAATATGCCAGCAATTTTTATTATTTAACATTTTGATTTCGTTTCTGAGGGGTCTGTTTT CTGTTTTGTAAGATTTTGGCACTATATATAACTGGGGATTAAGAACATTGCACCCGTGACTTCAGACAAA ATGAGAAGAACCTGGCAGAGCCGGCCCACTGCCAGGTTGTGTGGGACAGTCTGGGTCTGAGGTGAATTGC CTTTATTGTCTGCTGGTGTTTACAGAGTCCATCCTCGAACACTAAGCCATCCCACTCCTGTTTTAGAGAG GATCGGTTCATTACCATGCTTACAATCAGTCGGGTCTCTCCCACCTGTCTGCATAGAACGGAAGAATTAG GCACAGCCCGATTTTGGATAGTAAATGTGGATCGTTTGGCAT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:2645010), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 12164. Forward Primer - name:012164_F_IRAV08-11_D20_Stx12, sequence:TGCATGTGGAAAGAGCCA; Reverse Primer - name:012164_R_SP6_IRAV08-11_D20_Stx12, sequence:CATGCCAAACGATCCACA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12909 same embryo
	EMAGE:12911 same embryo
	EMAGE:12912 same embryo
	EMAGE:12908 same embryo
	EMAGE:12910 same embryo
	EurExpress:euxassay_011670 same experiment
	MGI:4828522 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source