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EMAGE:13014

Slc44a1 solute carrier family 44, member 1 ( MGI:2140592)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:13014 EMAGE:13014 EMAGE:13014 EMAGE:13014 EMAGE:13014
"Pseudo-wholemount" of euxassay_019727. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_019727_01 euxassay_019727_02 euxassay_019727_03 euxassay_019727_04
EMAGE:13014 EMAGE:13014 EMAGE:13014 EMAGE:13014 EMAGE:13014
euxassay_019727_05 euxassay_019727_06 euxassay_019727_07 euxassay_019727_08 euxassay_019727_09
EMAGE:13014 EMAGE:13014 EMAGE:13014 EMAGE:13014 EMAGE:13014
euxassay_019727_10 euxassay_019727_11 euxassay_019727_12 euxassay_019727_13 euxassay_019727_14
EMAGE:13014 EMAGE:13014 EMAGE:13014 EMAGE:13014 EMAGE:13014
euxassay_019727_15 euxassay_019727_16 euxassay_019727_17 euxassay_019727_18 euxassay_019727_19
EMAGE:13014 EMAGE:13014 EMAGE:13014 EMAGE:13014
euxassay_019727_20 euxassay_019727_21 euxassay_019727_22 euxassay_019727_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:13014Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
13014_wholemount_strong.wlz
13014_wholemount_moderate.wlz
13014_wholemount_weak.wlz
13014_wholemount_possible.wlz
13014_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:13014_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
rib
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 19 21 22 23 moderate expression: see section 20
hindlimb digit 1 phalanx
weak weak
regionalweak expression: see section 07 22
hindlimb digit 2 phalanx
weak weak
regionalweak expression: see section 07 08 22
hindlimb digit 3 phalanx
weak weak
regionalweak expression: see section 08 22
hindlimb digit 4 phalanx
weak weak
regionalweak expression: see section 08 22
hindlimb digit 5 phalanx
weak weak
regionalweak expression: see section 08 22
fibula
moderate moderate
regionalmoderate expression: see section 01 02 03
tibia
moderate moderate
regionalmoderate expression: see section 02 03
femur
strong strong
regionalstrong expression: see section 05 06 22 moderate expression: see section 23 weak expression: see section 04
thymus primordium
moderate moderate
regionalmoderate expression: see section 15 weak expression: see section 12 13 14
submandibular gland primordium
strong strong
regionalstrong expression: see section 07 moderate expression: see section 09 10 17 18 19 weak expression: see section 08 20
pancreas
moderate moderate
regionalmoderate expression: see section 09 10 11 weak expression: see section 16 17 18
pituitary gland
weak weak
regionalweak expression: see section 11 13 14 15 16
vibrissa
moderate moderate
regionalmoderate expression: see section 07 08 09 21 22
diencephalon lateral wall ventricular layer
moderate moderate
regionalmoderate expression: see section 13 14 weak expression: see section 15
cerebral cortex mantle layer
moderate moderate
regionalmoderate expression: see section 09 weak expression: see section 02 03 04 05 06 07 08 10 11 12 13 14 16 17 18 19 20 21 22
telencephalon ventricular layer
weak weak
regionalweak expression: see section 02 03 04 05 06 07 09 10 13 17 18 20 21 22
medulla oblongata basal plate mantle layer
moderate moderate
spottedmoderate expression: see section 12 13
midbrain ventricular layer
moderate moderate
regionalmoderate expression: see section 13 14 15 weak expression: see section 12
cornea
weak weak
regionalweak expression: see section 01 02 03 04 05 22 23
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 09 10 11 18 19 weak expression: see section 12 13 17 20
esophagus
moderate moderate
regionalmoderate expression: see section 13
hindgut
strong strong
regionalstrong expression: see section 14 15 16 17 18 19
midgut
strong strong
regionalstrong expression: see section 10 11 12 13 14 15 16 17 18 19 20 21
mandible
strong strong
regionalstrong expression: see section 04 05 06 07 08 09 10 11 12 13 16 17 18 19 20 21 22
maxilla
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 12 13 16 17 18 19 20 21 22
palatal shelf
weak weak
regionalweak expression: see section 12 13 15 16 17
liver
strong strong
spottedstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
bladder
moderate moderate
regionalmoderate expression: see section 13 14 15
testis
weak weak
regionalweak expression: see section 05 06 07 20 21
urethra of male
moderate moderate
regionalmoderate expression: see section 16 weak expression: see section 13 14 15
lung
weak weak
regionalweak expression: see section 03 04 05 06 07 08 09 10 11 14 15 16 17 18 19 20 21 22 23
orbito-sphenoid
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 22 23
clavicle
moderate moderate
regionalmoderate expression: see section 09 10 18 19
sternum
moderate moderate
regionalmoderate expression: see section 14 15
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T56050
Entity Detected:Slc44a1, solute carrier family 44, member 1 ( MGI:2140592)
Sequence:sense strand is shown

>T56050
CAGGTGCAGCAGCAAGACTAGTGTCAGGATACGACAGCTATGGAAATATCTGTGGGCAGAGAAATGCAAA
ACTGGAAGCAATACCGAACAGTGGTCTGGACCACACCCACCGGAAGTATGTGTTCTTTTTGGACCCATGC
AACCTGGACTTGATAAACAGGAAGATCAAGTCTATAGCACTGTGCGTAGCAGCATGTCCTCGACAGGAAT
TGAAAACCTTGAGTGACGTCCAGAAGTTTGCAGAGATCAATGGCTCAGCACTATGTAGCTACAACATAAA
GCCTTCTGAATATACTCTGACTTCAAAATCCTCTGGCTTTTGCCCCAAGCTACCAGTACCAGCCAGTGCA
CCTATTCCATTCTTCCATCGCTGTGCTCCTGTGAACATTTCCTGCTATGCCAAGTTTGCAGAGGCCCTGA
TCACCTTTGTCAGTGACAATAGTGTCTTACACAGGCTGATTAGTGGAGTAATGACCAGCAAAGAAATTAT
ATTGGGACTTTGCTTGTTATCACTAGTTCTGTCCATGATCCTGATGGTGATTATCAGGTATATATCAAGA
GTGCTAGTGTGGATTCTAACAGTTCTGGTCATACTGGGTTCACTTGGTGGCACGGGTGTATTATGGTGGC
TGTATGCAAAGCAAAGAAGGTCCCCCAAAGAAGCAGTTATTCCTGAGCAGCTTCAGATAGCTGAGGACAA
TCTGCGGGCGCTCCTCATCTATGCCATTTCAGCGACAGTGTTCACTGTCATTCTCTTCCTGATCATGCTG
GTTATGCGCAAGCGTGTGGCTCTCACCATCGCCTTGTTCCACGTGGCTGGCAAGGTCTTCATACACTTGC
CTCTGTTAGTCTTCCAGCCCTTCTGGACGTTTTTTGCTCTCGTCTTATTTTGGGCATACTGGATAATGAC
ACTTCTCTTCCTTGGCACTACTGGCAGTGCTGTTCAGAATGAGCAAGGCTTCGTGGAGTACAAAATCTCC
GGGCCT
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:CAGGTGCAGCAGCAAGACTA; Reverse Primer - name:unspecified, sequence:AGGCCCGGAGATTTTGTACT. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:13015 same embryo
 EurExpress:euxassay_019727 same experiment
 MGI:4828233 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS