EMAGE mouse gene expression spatial database

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EMAGE:13048

Ier5l immediate early response 5-like ( MGI:1919750)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013772. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013772_01	euxassay_013772_02	euxassay_013772_03	euxassay_013772_04

euxassay_013772_05	euxassay_013772_06	euxassay_013772_07	euxassay_013772_08	euxassay_013772_09

euxassay_013772_10	euxassay_013772_11	euxassay_013772_12	euxassay_013772_13	euxassay_013772_14

euxassay_013772_15	euxassay_013772_16	euxassay_013772_17	euxassay_013772_18	euxassay_013772_19

euxassay_013772_20	euxassay_013772_21	euxassay_013772_22	euxassay_013772_23	euxassay_013772_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13048_wholemount_strong.wlz

13048_wholemount_moderate.wlz

13048_wholemount_weak.wlz

13048_wholemount_possible.wlz

13048_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13048_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hindlimb digit 1 phalanx

moderate

regional

moderate expression: see section 03 04 23 24

hindlimb digit 2 phalanx

moderate

regional

moderate expression: see section 03 04 21 22

hindlimb digit 3 phalanx

moderate

regional

moderate expression: see section 03 04 05 21 22

hindlimb digit 4 phalanx

moderate

regional

moderate expression: see section 04 05 06 21 22

hindlimb digit 5 phalanx

moderate

regional

moderate expression: see section 05 06 21 22

vertebral axis musculature

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 17 19 20 21 22 23 24

thymus primordium

moderate

regional

moderate expression: see section 12 13 14 15 16

sublingual gland primordium

strong

regional

strong expression: see section 11 17 18

submandibular gland primordium

strong

regional

strong expression: see section 08 09 10 19 20

pancreas

moderate

regional

moderate expression: see section 10 11 12 13 17

thyroid gland

moderate

regional

moderate expression: see section 11 12 15

vibrissa

moderate

regional

moderate expression: see section 09 10 22 23 24 weak expression: see section 06 07 08

hypothalamus ventricular layer

strong

regional

strong expression: see section 12 13 14 15 16

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 12 13 14 15

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 12 13 14 18 19

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 16 17 18 19 20 21 22 23 24

rest of cerebellum marginal layer

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 14 16 17 18 19 20 21 22

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 14 17 18 19 20 21 22

midbrain ventricular layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18

pharyngo-tympanic tube

moderate

regional

moderate expression: see section 01 05 22 weak expression: see section 02 03 04

neural retina

moderate

regional

moderate expression: see section 01 02 03 04 05 24

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 10 11 12 13 14 16 19 20 21

esophagus

moderate

regional

moderate expression: see section 13

stomach

weak

regional

weak expression: see section 03 04 05 06 07 08 09 10 11

hindgut

weak

regional

weak expression: see section 15

midgut

weak

regional

weak expression: see section 07 08 09 10 11 12 13 14 15 16 17 18

lower jaw incisor

moderate

regional

moderate expression: see section 12 13 16 17

lower jaw molar

moderate

regional

moderate expression: see section 07 08 09 21

upper jaw incisor

moderate

regional

moderate expression: see section 12 13 14 17

upper jaw molar

moderate

regional

moderate expression: see section 07 08 09 21 22

liver

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

metanephros

moderate

regional

moderate expression: see section 07 08 09 10 11 18 19 20 21 22

testis

weak

regional

weak expression: see section 06 07 08 09 21 22 23

lung

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

trachea

moderate

regional

moderate expression: see section 13 14

axial skeleton tail region

weak

regional

weak expression: see section 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6234
Entity Detected:	Ier5l, immediate early response 5-like ( MGI:1919750)
Sequence:	sense strand is shown >T6234 GGGGAGCGTGAGCCTGCGGGTGGCGCGCAACGCATGGTGGCGACTTCTCTCGGAGTGTCGCCGACCCGCC GACCCGGGCTCGGTTCCCTGTGCCTGGCTCGCCCGGGCGACCAACTGGAGACACACGCAAGGAGCAGCAT TCGCAGCACCAGAGGCGGCTGCTCCGCCGCCCGCGTCTCCGCGGGAGCATGGAGTGCGCCCTGGACGCCC AGAGCCTGATCAGCATCTCCCTGCGCAAGATCCACAGCTCCCGGACCCAGCGCGGCGGCATCAAGCTGCA CAAGAACCTCCTGGTGTCCTACGTGCTCCGCAACGCGCGCCAGCTCTACCTGAGCGAGCGTTACGCCGAG CTCTACCGGCGCCAGCAGCAGCAGCAGCAACAGCAGCCGCCCCACCACCAGCACCAGCACCTCGCTTACG CGGCGCCCGGAATGCCGGCCAGCGCGGCCGACTTCGGCCCGCTCCAACTTGGCGGCGGCGGGGACGCGGA GGCGCGCGAGCCGGTTGCCCGGCACCAGCTGCACCAGCTCCACCAGCTTCACCAGCTGCACCTCCAGCAG CAGCTGCACCAACACCAACACCCGGCGCCCAGGGGCTGCACGGCGGCGGCGCCGGTGGCGGTGGCCGGGG CGCCCGCGGGCTGCGCGGGGGCGCTCTCGGAGCTGCCCGGGTGCGCTGCGCTCCAGCCGCCGCACGGCGC GCCCCACCGCGGGCAGCACTTGGAGCCGCTGCAGCCGGGTCCCGCGCCGCTGCCGCCGCCCGCGCCCGCC GCGCTCTGCCCGCGGGACCCTCGCGTCCCGGCCGCTTGCTCTGCGCCCTCGGTGCTTCCGGGGGCTGCCC CTTCGACCGTCGCTGCCTCCTCTCCGCCCGCCTCTACAGCCCCTTCCTCATCCACTGGCTTCTACCGGGG CGCGTACCCGGCCCCCTCGGACTTCGGCGTGCACTGCAGCAGCCAGACCACCGTGCTGGACCTGGACACT CACGTGGTGACCACGGTAGAAAACGGCTACTTGCACCAGGACTGCTGCGCCTCCGCCCACTGCCCCTGCT GTGGCCAGGGCGCTCCGGGACCCGGTCTGGCGTCCGCCGCTGGTTGCAAGCGCAAGTATTACCCTGGCCA GGAGGAAGAGGACGACGAAGAGGACGCGGGCGACCTGGGAGCCGAGCCCCCCGGGGGTACCCCATTCGCC CCCTGTAAGCGCGCCCGCTTCGAGGACTTCTGCCCGGACTCGTCCCCGGACGCGTCCAACATCTCAAACT TGATCTCCATCTTTGGCTCGGGCTTCTCGGGGCTGGTGAGCCGACAGCCGGACTCCTCGGAGCAGCCGCC GCCGCTCAACGGGCAGCTGTGCGCCAAGCAGGCGCTCGCCAGCCTCGGCGCCTGGACTCGAGCCATTGTC GCCTTCTAGGGACCCCCGAGGGCATGGGGACCCTGGGCCCCGCGGGGCTGGGGCCAGACAAAGACTCGGC CAAGGGGCGATAGGGAGCGAGCGGGTCCTGGCCACTCGGGGCTGAGCTGGGGACGAGCAGAGGCTGATGT TTTATAAATTGTAAAATAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3499467 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3499467 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13049 same embryo
	EMAGE:13047 same embryo
	EMAGE:13046 same embryo
	EurExpress:euxassay_013772 same experiment
	MGI:4825505 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source