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EMAGE:13690

Znhit3 zinc finger, HIT type 3 ( MGI:3051596)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:13690 EMAGE:13690 EMAGE:13690 EMAGE:13690 EMAGE:13690
"Pseudo-wholemount" of euxassay_019512. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_019512_01 euxassay_019512_02 euxassay_019512_03 euxassay_019512_04
EMAGE:13690 EMAGE:13690 EMAGE:13690 EMAGE:13690 EMAGE:13690
euxassay_019512_05 euxassay_019512_06 euxassay_019512_07 euxassay_019512_08 euxassay_019512_09
EMAGE:13690 EMAGE:13690 EMAGE:13690 EMAGE:13690 EMAGE:13690
euxassay_019512_10 euxassay_019512_11 euxassay_019512_12 euxassay_019512_13 euxassay_019512_14
EMAGE:13690 EMAGE:13690 EMAGE:13690 EMAGE:13690 EMAGE:13690
euxassay_019512_15 euxassay_019512_16 euxassay_019512_17 euxassay_019512_18 euxassay_019512_19
EMAGE:13690 EMAGE:13690 EMAGE:13690 EMAGE:13690
euxassay_019512_20 euxassay_019512_21 euxassay_019512_22 euxassay_019512_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:13690Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
13690_wholemount_strong.wlz
13690_wholemount_moderate.wlz
13690_wholemount_weak.wlz
13690_wholemount_possible.wlz
13690_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:13690_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
thymus primordium
weak weak
regionalweak expression: see section 12 13 14 15 16
submandibular gland primordium
weak weak
regionalweak expression: see section 07 08 09 10 17 18 19 20 21
pancreas
weak weak
regionalweak expression: see section 09 10 11 12 16 17 18
vibrissa
weak weak
regionalweak expression: see section 06 07 20 21 22
liver
moderate moderate
regionalmoderate expression: see section 01 02 03 05 weak expression: see section 04 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T55232
Entity Detected:Znhit3, zinc finger, HIT type 3 ( MGI:3051596)
Sequence:sense strand is shown

>T55232
ATGGCGTCGCTGAATTGTAGGACTGCGGTCTGTGTGGTCTGTTTGGAGAAGCCGAAATACCGTTGCCCGA
CTTGCCGCGTGCCCTACTGTTCGGTCCCCTGTTTTCAGAAGCACAAAGAGCAGTGCAGCTCTGAAGCCCG
ACCTGTTGAGAAGAGAAGAGCAGGGCCTCCTGTGAGGTCTGAGGAGAGCAAAGATGATGACTCCTCCGTA
GCTGATTTCCTCAACAGTGATGAGGAAGAAGACAGAGTGTCTCTGCAGAATTTAAAGAATCTAGGTGAAT
CGGAAACTTTAAGAAGCTTGCTGCTGAACCCACACCTGAGGCAGTTGATGATTAGCCTCGATCAGGGTGA
CAACAAAGCAAAGCTGATGCGAGCCTGTATGCAGGAGCCCCTTTTCGTGGAGTTTGCAGACTGCTGTTTA
GGAATCGTGGAACCATCCCAGAAGAGGGATTCCTAAGACTGGATGTGTTCTCTGCTCAAGTTACCTGCTG
CTGCTCCAAGAGGTGGCTGTTTTCAAGGCCACCATACGCAGCATGCACACGTGGGTCTTCCTCTACCACA
TGGCTCGCTGCAGCAACTTCATGAAGAGGAAACTTGTAGCCC
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:ATGGCGTCGCTGAATTGTAG; Reverse Primer - name:unspecified, sequence:GGGCTACAAGTTTCCTCTTCA. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:13693 same embryo
 EMAGE:13694 same embryo
 EMAGE:13692 same embryo
 EMAGE:13691 same embryo
 EurExpress:euxassay_019512 same experiment
 MGI:4829362 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS