EMAGE mouse gene expression spatial database

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EMAGE:13767

Cd164 CD164 antigen ( MGI:1859568)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019262. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019262_01	euxassay_019262_02	euxassay_019262_03	euxassay_019262_04

euxassay_019262_05	euxassay_019262_06	euxassay_019262_07	euxassay_019262_08	euxassay_019262_09

euxassay_019262_10	euxassay_019262_11	euxassay_019262_12	euxassay_019262_13	euxassay_019262_14

euxassay_019262_15	euxassay_019262_16	euxassay_019262_17	euxassay_019262_18	euxassay_019262_19

euxassay_019262_20	euxassay_019262_21	euxassay_019262_22	euxassay_019262_23	euxassay_019262_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13767_wholemount_strong.wlz

13767_wholemount_moderate.wlz

13767_wholemount_weak.wlz

13767_wholemount_possible.wlz

13767_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13767_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 weak expression: see section 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20 21 22 23 24

thymus primordium

moderate

regional

moderate expression: see section 12 13 14 15 16

pancreas

moderate

regional

moderate expression: see section 09 10 11 12 15 16 17

pituitary gland

moderate

regional

moderate expression: see section 11 12 13 14 15 16

vibrissa

moderate

regional

moderate expression: see section 05 06 07 19 20 21

hypothalamus ventricular layer

strong

homogeneous

strong expression: see section 11 13 14 moderate expression: see section 12

diencephalon lateral wall ventricular layer

strong

homogeneous

strong expression: see section 11 13 14 moderate expression: see section 12

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 09 10 11 15 16

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 13 14 15 16 17 18 19 20 21 22 23 moderate expression: see section 12

medulla oblongata basal plate ventricular layer

strong

homogeneous

strong expression: see section 09 10 11 12 13 14 15 16 17 18 19

rest of cerebellum ventricular layer

strong

homogeneous

strong expression: see section 05 06 07 17 18 19 21 22 moderate expression: see section 04 08 09 20

pons ventricular layer

strong

homogeneous

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19

midbrain ventricular layer

strong

homogeneous

strong expression: see section 13 14 15 16 17 18 moderate expression: see section 12

ventral grey horn

moderate

regional

moderate expression: see section 13 14 weak expression: see section 11 12 15 16 17

spinal cord ventricular layer

moderate

regional

moderate expression: see section 14

cornea epithelium

strong

regional

strong expression: see section 01 02 24 moderate expression: see section 03 23

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 14 18 weak expression: see section 09 10 11 15 16 17

tongue muscle

moderate

regional

moderate expression: see section 12 13 14 weak expression: see section 11 15

lower jaw incisor

weak

regional

weak expression: see section 11 12 15 16

lower jaw molar

weak

regional

weak expression: see section 19 20

upper jaw incisor

weak

regional

weak expression: see section 11 15 16

upper jaw molar

weak

regional

weak expression: see section 06 20

lung

moderate

regional

moderate expression: see section 06 10 11 13 15 16 17 18 19 20 weak expression: see section 07 08 09 12 21 22 23 24

nucleus pulposus

strong

regional

strong expression: see section 13 weak expression: see section 14

orbito-sphenoid

strong

regional

strong expression: see section 01 02 03 04 21 22 23 moderate expression: see section 05 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6653
Entity Detected:	Cd164, CD164 antigen ( MGI:1859568)
Sequence:	sense strand is shown >T6653 CGGACGCCTGGGCTGAAGACACAATGTCGGGCTCCTCCCGCCGACTGCTCTGGGCCGCCACCTGCCTCGC CGTGCTCTGCGTCTCGGCCGCGCAGCCCAACATCACCACCCTGGCTCCCAACGTGACCGAGGTGCCGACC ACGACCACCAAAGTGGTCCCGACGACGCAAATGCCCACCGTGCTACCAGAAACCTGTGCGAGCTTCAACA GCTGTGTTTCCTGTGTTAATGCCACCTTTACTAATAATATTACCTGCTTTTGGTTACATTGCCAAGAAGC AAATAAGACCTATTGTGCAAATGAACCATTAAGTAATTGTTCCCAGGTGAACCGCACTGACTTGTGTTCT GTAATACCTCCTACCACTCCAGTGCCAACCAATTCTACAGCTAAGCCCACAACTCGGCCTTCCTCTCCTA CACCTACTCCCTCAGTTGTCACATCAGCAGGGACAACAAATACCACACTGACTCCAACCTCCCAGCCTGA GCGGAAGTCGACCTTTGATGCAGCCAGCTTCATTGGAGGGATCGTCCTTGTCTTGGGTGTGCAGGCTGTA ATTTTCTTTCTCTATAAGTTCTGCAAATCTAAAGAACGAAATTACCATACTCTGTAAACAGACCTGTTAA ATTAATGTGGACTGGCAGTTAACTTGTGCAACTCACTGAAACCAAAATATTGAAGAAGTCCCACATGGAA GACGCTGTTCCAGGATCTTTACCTTTCCAAAGGATGCATACAGGATATTTTGGAGCATCATCCTCGAAGA AAAGTCAGTTAAACCATTTAGTTCATCAGGAATATTTAAAATATTCTGCATGAATCCGTGTGGCTGTCGT CTTTGTTTACCATGGCTGCTGCTGTGGGACTGTTTTTTCCTCTGACATGCCAAATGTAACTTCTGTAAGT GGTGGAGAGTGCTGTCCTGTGTAGGCAGCTTCTGACTGCAGTTGAAATGTAGTCCCCTAGAATCCTAAAG GCTGTTATTCTCATCCTAGTTCCAGGTGTAACTTGGTGGCCAGAATTGAGAAGAGTGCCAAATGACCACC CGTCAGGCAGATTGAGGGCCATCACTTCAAACATAGATTAAATTTGTTTAGCACTAAACTATCCCTAGAG TAAATTTTTATGCTTGACAAGTTGTATTTTATATATTAGAAATAAGATGCCACACAGGGCAATCCAGATT TAGATTAAAGAAAGGATACACAGACATTAATCTGCAACAGGTCTTGTTCATACTCATAAAGTGTACCGTA TTAAGAAATCAGAACAGTGCCTTAAGACAGACTGAGACGTCGTGAAAAATGTGTTCACACTGTGGGAGGC AAAGCTTGGTCTGAGAATGTAACTGTTGATGTGAGCAGCAGAAATCTGCTTATAGATAACATACTGTTAA TATTTAATTAAAAGCATTGTTTTTTCTAGAGCTGAGAAATAGAAGCTAAAGAATAATTAGAGCTGAAAGG CTTCCTTATATCAAACCATCTAACATTTTTCTTAGGGCTGTTAAACTAGACATAAAATTGATTTATCTTT CTCTCTGTACACAAAGTATCCAAATATTATACCTTAAGGATTCCTACCAGGTTTTCTTGAAAAGTGGCTT TTACATGAGGCAAGAAGGAAGTAGTTGTAGATAGAATCATTTTCTAGTTCTTTAAATGAGACAATCACTT TGTTTTCTAATAGATGTGACCTGTGTAACTTAAAAAATCTGAGGGTTTGAGAGAGGTTGCTCTAGATTTC CAATGACGATACCCTGGCTGAATAGGCTTTTCTCTTCCTTGGGCCTTTACTTCTTTTCTTCCTCCTTGGT TTCAGTTCCCATGTGCTATCATTATAATCATGCAGACTTTTACTTCAGTTGCTTCTGAAAGCACTAAGTT TTCAGTTTTGGAGTTACTTCTCCCTTGCCTCTGACACACTGCACACGTCTAGAGGCTGTAATTGGAAATA GGCTTTTGGTTTCCTGTGCCATTCAAGTTTGGGGCTTTCATTTGGTTACAGAGTTCAGCGCTTGGTTCCC AGGGTTTATATACGAAACAGCGGATTGCTGTGGGCTGGTTCATAAAGTGAAACTAATGAATCTATCTCTT TGTCACTTGCTGGTACAGCTAGTTTGTTTCACTTGCACATTTGTACATATATCTAATGTTTTCAAGTGCC TTACTTGTTTAAACTCTGACTTCAAAGGTTTTTGGGGAAAGATAGGTTTACCTCACAGTTATGTTTCCAT TGTAATTTCTAGGTTCCTCACAAAAATCTATTTCAGTGCTGTAGCTGTTAGTTTTAAGTGAGTGCTAAAG TTCTTTTAAAAGTACACAGAATTTCCATTCCTTATAAAGGTGGCAAAATTGGTTTGTCATTTACCTCCAT GTGACTCCTTGCCCCATATAAATGTCAGCATATGTAGCAAGTTTTCTAAGAATGTAACTTAATTTGAAGC TCCTGAAATTTTGAGCACTGCTCTAACATTGTAGGAAGTTAGTGACATTTTGCAAGTCTCTACAAAAGCT CTTGCCACAGAATTCACTGCTGCCCTGACTCTTCACCATCTTTCTTTAGCAAGGTATTTTGAGCCATTAA TGATTTTGGGTTTTTTTAATCCAGAAGGTATATAAAAGCCTTTTCAGAGTTTTCATCTGATTTGTCCTGA GGATCTAGTTCTATCAAATACCTTATTTTACCTTTGCCATACTTGCTGCACAAGCAGACACTGCTGGACT TAGAAGTTTCTATTTGAGTTCCTTAAACTACAAAACTAATATGTATGTGTACCAAACTGATTTAAAATAA ATGTTTATTAGTCTCTTGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3600535 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3600535 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13768 same embryo
	EurExpress:euxassay_019262 same experiment
	MGI:4823716 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source