EMAGE mouse gene expression spatial database

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EMAGE:13787

Irx3 Iroquois related homeobox 3 (Drosophila) ( MGI:1197522)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019561. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019561_01	euxassay_019561_02	euxassay_019561_03	euxassay_019561_04

euxassay_019561_05	euxassay_019561_06	euxassay_019561_07	euxassay_019561_08	euxassay_019561_09

euxassay_019561_10	euxassay_019561_11	euxassay_019561_12	euxassay_019561_13	euxassay_019561_14

euxassay_019561_15	euxassay_019561_16	euxassay_019561_17	euxassay_019561_18	euxassay_019561_19

euxassay_019561_20	euxassay_019561_21	euxassay_019561_22	euxassay_019561_23	euxassay_019561_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13787_wholemount_strong.wlz

13787_wholemount_moderate.wlz

13787_wholemount_weak.wlz

13787_wholemount_possible.wlz

13787_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13787_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

interdigital region between hindlimb digits 1 and 2

moderate

regional

moderate expression: see section 04 05 23

interdigital region between hindlimb digits 2 and 3

moderate

regional

moderate expression: see section 05 23

interdigital region between hindlimb digits 3 and 4

moderate

regional

moderate expression: see section 05 22

interdigital region between hindlimb digits 4 and 5

moderate

regional

moderate expression: see section 22

vibrissa

weak

regional

weak expression: see section 06 07 21 22

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 13 14 moderate expression: see section 09 10 11 12 15 16 17

pons mantle layer

moderate

regional

moderate expression: see section 06 07 18 19

pons ventricular layer

moderate

regional

moderate expression: see section 09 17 weak expression: see section 08 10 14 15 16

midbrain ventricular layer

strong

regional

strong expression: see section 06 07 09 10 11 12 13 14 15 16 17 18 moderate expression: see section 08

intermediate grey horn

strong

regional

strong expression: see section 14 15 moderate expression: see section 13

saccule

strong

regional

strong expression: see section 05 06 07 17 18 19 20 21

cochlea

strong

regional

strong expression: see section 09

neural retina

strong

regional

strong expression: see section 01 02 03 23 24

stomach

moderate

regional

moderate expression: see section 05 06 weak expression: see section 02 03 04

metanephros

moderate

regional

moderate expression: see section 09 10 17 18 19 weak expression: see section 07 08

ovary

strong

regional

strong expression: see section 06 07 moderate expression: see section 19 20 21

lung

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T55089
Entity Detected:	Irx3, Iroquois related homeobox 3 (Drosophila) ( MGI:1197522)
Sequence:	sense strand is shown >T55089 AGCCCAAGATCTGGTCACTGGCCGAGACGGCCACCAGCCCGGACAACCCACGCCGCTCGCCTCCCGGAGC CGGAGGTTCGCCTCCCGGCGCAGCCGTTGCGCCCCCGACGCTGCAGCTCTCGCCCGCGGCCGCTGCAGCG GCTGCAGCGGCACACAGACTCGTGTCGGCGCCGCTTGGCAAATTCCCCGCTTGGACCAACAGGCCTTTCC CAGGGCCGCCGGCCGGTCCGCGGCCGCACCCGCTGTCCATGCTGGGCTCGGCCCCACAGCACCTGCTGGG ACTTCCCGGAGCCGCAGGTCATCCCGCTGCGGCCGCAGCCGCCTATGCTCGGCCTGCCGAGCCGGAGAGT GGAACAGATCGCTGTAGTGCCTTGGAAGTGGAGAAAAAGTTACTCAAGACAGCTTTCCAGCCGGTGCCAA GGCGGCCACAGAACCATCTGGACGCTGCTCTGGTCTTATCAGCTCTCTCCTCGTCTTAACTTTTCAACCA TTTTTAAATCGTTGTACTAATTGTAAAAAACAAAACAAAACAAAATCGCTCTGTATAGTGACGAGTTGCA AGCATGTCTGTGTATGATTACCTTAAAAGAAACACTAAACAACAACAACAACAACAACAACAACAGGAAA GGACAAAAGAAAGAAAGAAAGCCAGTCCCCCCACCTTGGCCCTCCCCCAAGTCACCCCACATTTAGATTG TGAGGTTCCTTCGTTGTGTTGATTTCGGGGGGGGGGGGAGTGGGGTTACGGAGAGAACAAGCCTGTCTGA CTCTTTTTGTATATACGGGAAAATGTACATACGTGTGAACCAAATTGTACAAGAAAGTATCTATTTTTTG GCTAAATAAATGAGCTGTTGCCACTTTTGAC
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:AGCCCAAGATCTGGTCACTG; Reverse Primer - name:unspecified, sequence:GTCAAAAGTGGCAACAGCTC. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	MGI:4825624 same experiment
	EurExpress:euxassay_019561 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source