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EMAGE:13812

Mrps27 mitochondrial ribosomal protein S27 ( MGI:1919064)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:13812 EMAGE:13812 EMAGE:13812 EMAGE:13812 EMAGE:13812
"Pseudo-wholemount" of euxassay_019261. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_019261_01 euxassay_019261_02 euxassay_019261_03 euxassay_019261_04
EMAGE:13812 EMAGE:13812 EMAGE:13812 EMAGE:13812 EMAGE:13812
euxassay_019261_05 euxassay_019261_06 euxassay_019261_07 euxassay_019261_08 euxassay_019261_09
EMAGE:13812 EMAGE:13812 EMAGE:13812 EMAGE:13812 EMAGE:13812
euxassay_019261_10 euxassay_019261_11 euxassay_019261_12 euxassay_019261_13 euxassay_019261_14
EMAGE:13812 EMAGE:13812 EMAGE:13812 EMAGE:13812 EMAGE:13812
euxassay_019261_15 euxassay_019261_16 euxassay_019261_17 euxassay_019261_18 euxassay_019261_19
EMAGE:13812 EMAGE:13812 EMAGE:13812 EMAGE:13812 EMAGE:13812
euxassay_019261_20 euxassay_019261_21 euxassay_019261_22 euxassay_019261_23 euxassay_019261_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:13812Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
13812_wholemount_strong.wlz
13812_wholemount_moderate.wlz
13812_wholemount_weak.wlz
13812_wholemount_possible.wlz
13812_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:13812_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
axial musculature
moderate moderate
regionalmoderate expression: see section 06 07 08 09 22 23 weak expression: see section 24
adrenal gland
moderate moderate
regionalmoderate expression: see section 09 10 11 19 20 21 weak expression: see section 12
thymus primordium
strong strong
regionalstrong expression: see section 13 14 17 18 moderate expression: see section 15 16
submandibular gland primordium
strong strong
regionalstrong expression: see section 08 09 10 11 19 20 21 22 23
pancreas
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13 18
thyroid gland
weak weak
regionalweak expression: see section 13 14 18
pituitary gland
weak weak
regionalweak expression: see section 12 13 14 15 16 17
vibrissa
moderate moderate
regionalmoderate expression: see section 07 08 09 10 20 21 22 23 weak expression: see section 05 06
olfactory cortex ventricular layer
moderate moderate
regionalmoderate expression: see section 11 12 17 18 19
telencephalon ventricular layer
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 15 16 17 18 19 20 21 22 23 weak expression: see section 24
medulla oblongata basal plate mantle layer
weak weak
regionalweak expression: see section 10 11 18 19
rest of cerebellum marginal layer
weak weak
regionalweak expression: see section 07 08 10 11 13 14 15 16 17 18 19 20 21 22 23
rest of cerebellum ventricular layer
weak weak
regionalweak expression: see section 07 08 09 10 11 13 14 17 18 19 20 21 22 23
pons mantle layer
weak weak
regionalweak expression: see section 09 20
midbrain ventricular layer
moderate moderate
regionalmoderate expression: see section 13 14 15 16 17 18 weak expression: see section 11 12 19
facial vii ganglion
weak weak
regionalweak expression: see section 06 07 22 23
glossopharyngeal ix ganglion
weak weak
regionalweak expression: see section 08 09 21
trigeminal v ganglion
weak weak
regionalweak expression: see section 04 05 06 07 08 09 10 11 19 20 21 22 23
vagus x ganglion
weak weak
regionalweak expression: see section 10 20
cervico-thoracic ganglion
weak weak
regionalweak expression: see section 11 19
cervical ganglion
weak weak
regionalweak expression: see section 11 19
thoracic ganglion
weak weak
regionalweak expression: see section 14 15
dorsal root ganglion
weak weak
regionalweak expression: see section 11 13 18 19 20 21
pharyngo-tympanic tube
moderate moderate
regionalmoderate expression: see section 01 02 03 weak expression: see section 04 05 06
neural retina
weak weak
regionalweak expression: see section 01 02 03 04
nasal cavity olfactory epithelium
weak weak
regionalweak expression: see section 09 10 11 17 18 19 20 21
stomach
moderate moderate
regionalmoderate expression: see section 04 09 10 weak expression: see section 02 03 05 06 07 08
midgut
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 weak expression: see section 06 07
lower jaw incisor
moderate moderate
regionalmoderate expression: see section 17 18 weak expression: see section 12 13 14 16
lower jaw molar
moderate moderate
regionalmoderate expression: see section 09 20 21 weak expression: see section 08
upper jaw incisor
moderate moderate
regionalmoderate expression: see section 17 18 weak expression: see section 12 13 14 16
upper jaw molar
moderate moderate
regionalmoderate expression: see section 09 20 21 weak expression: see section 08
liver
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 weak expression: see section 24
metanephros
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 18 19 20 21 22 23
testis
strong strong
regionalstrong expression: see section 06 07 08 moderate expression: see section 09 22 23 24
lung
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 weak expression: see section 23 24
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T465
Entity Detected:Mrps27, mitochondrial ribosomal protein S27 ( MGI:1919064)
Sequence:sense strand is shown

>T465
CTCGCCTTTGCCTCGCAGGTAAAAGATGCCTGCTTTCTGCAGCGTACGTGGACAGCCACCAGTGGGAAGC
CAGAGAGAAGGAGGAATACCATCTGGCTGATCTTGCATCCTTAATGGACAAAGCGTATGAAAGAAAATTG
CCTGTTAGTTCTTTATCAATATCACGGTTTGTGGACAACATTGCATCCCGAGAAGATCTAGATTCTGCAG
AGTACTATCTTTACAAATTTCGACACAGTCCCAACTGCTGGTACCTAAGAGACTGGACCATCCACAGCTG
GATTAGACAGTGTCTGAAATACGGAGCACAAGACAAAGCCTTGTATACACTTGTTAATAAGGTTCAGTAT
GGAATTTTTCCAGATAACTTTACATTCAACTTACTGATGGATTATTTCATAAAGAAAGAAAAACTCCCCG
CCTGTGAGGCCGAGGACCTGGCCACCTACGAGCAGAAACTCC
Notes:The probe template was PCR amplified from IMAGE:1450742 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1450742 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:13813 same embryo
 EMAGE:13811 same embryo
 EurExpress:euxassay_019261 same experiment
 MGI:4826454 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS