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EMAGE:13828

Foxf2 forkhead box F2 ( MGI:1347479)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:13828 EMAGE:13828 EMAGE:13828 EMAGE:13828 EMAGE:13828
"Pseudo-wholemount" of euxassay_019593. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_019593_01 euxassay_019593_02 euxassay_019593_03 euxassay_019593_04
EMAGE:13828 EMAGE:13828 EMAGE:13828 EMAGE:13828 EMAGE:13828
euxassay_019593_05 euxassay_019593_06 euxassay_019593_07 euxassay_019593_08 euxassay_019593_09
EMAGE:13828 EMAGE:13828 EMAGE:13828 EMAGE:13828 EMAGE:13828
euxassay_019593_10 euxassay_019593_11 euxassay_019593_12 euxassay_019593_13 euxassay_019593_14
EMAGE:13828 EMAGE:13828 EMAGE:13828 EMAGE:13828 EMAGE:13828
euxassay_019593_15 euxassay_019593_16 euxassay_019593_17 euxassay_019593_18 euxassay_019593_19
EMAGE:13828 EMAGE:13828 EMAGE:13828 EMAGE:13828 EMAGE:13828
euxassay_019593_20 euxassay_019593_21 euxassay_019593_22 euxassay_019593_23 euxassay_019593_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:13828Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
13828_wholemount_strong.wlz
13828_wholemount_moderate.wlz
13828_wholemount_weak.wlz
13828_wholemount_possible.wlz
13828_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:13828_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
forelimb digit 1 phalanx
strong strong
regionalstrong expression: see section 02 03 04
forelimb digit 2 phalanx
strong strong
regionalstrong expression: see section 02
hindlimb digit 1 phalanx
strong strong
regionalstrong expression: see section 02
hindlimb digit 2 phalanx
strong strong
regionalstrong expression: see section 01 02 24
hindlimb digit 3 phalanx
strong strong
regionalstrong expression: see section 02 24 moderate expression: see section 03
hindlimb digit 4 phalanx
strong strong
regionalstrong expression: see section 04 05 24 moderate expression: see section 03
saccule
strong strong
regionalstrong expression: see section 06 09 17 18 19 moderate expression: see section 07 weak expression: see section 08
esophagus
moderate moderate
regionalmoderate expression: see section 14 weak expression: see section 13
tongue
strong strong
regionalstrong expression: see section 11 17 18 moderate expression: see section 12 13 14 15 16
stomach
moderate moderate
regionalmoderate expression: see section 06 07 weak expression: see section 05 08 09 10 11 12
hindgut
moderate moderate
regionalmoderate expression: see section 14 15 weak expression: see section 16
midgut
moderate moderate
regionalmoderate expression: see section 12 13 14 15 weak expression: see section 11 16 17 18 19 20 21
palatal shelf
strong strong
regionalstrong expression: see section 09 10 11 18 moderate expression: see section 14 15 16 19
bladder
strong strong
regionalstrong expression: see section 14 15 moderate expression: see section 16
genital tubercle of male
strong strong
regionalstrong expression: see section 13 14 15 16 17 18 19
urethra of male
strong strong
regionalstrong expression: see section 15 16
larynx
strong strong
regionalstrong expression: see section 14 moderate expression: see section 13 15
lung
moderate moderate
regionalmoderate expression: see section 05 06 07 09 10 11 14 15 17 18 19 weak expression: see section 08 12 13 16 20 21 22 23 24
axial skeleton tail region
strong strong
regionalstrong expression: see section 16 17 18
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T55055
Entity Detected:Foxf2, forkhead box F2 ( MGI:1347479)
Sequence:sense strand is shown

>T55055
GGTTATGGTGGCCTCGACATGATGCCCGCGGGCTATGATACAGGGGCGGGTGCTCCGGGCCACGCGCATC
CACAGCACCTCCACCACCACCACGTCCCCCACATGTCGCCCAACCCGGGCTCCACCTATATGGCCAGCTG
CCCGGTGCCCGCAGGTCCTGCGGGCGTCGGTGCGGCAGCGGGTGGCGGCGGTGGCGGCGGGGACTATGGG
CCGGACAGCAGCAGCAGCCCTGTGCCCTCATCCCCGGCTATGGCAAGCGCCATTGAGTGTCACTCGCCCT
ACACTAGCCCTGCGGCACATTGGAGCTCGCCTGGCGCTTCACCTTACCTCAAGCAGCCGCCTGCCCTGAC
GCCAAGCAGTAATCCCGCGGCCTCTGCTGGTCTGCACCCCAGCATGTCTTCCTACTCGTTGGAGCAGAGC
TACTTGCACCAGAACGCCCGCGAGGATCTCTCAGTCGGACTGCCCCGTTACCAGCACCACTCCACTCCAG
TGTGCGACAGGAAAGATTTCGTCCTCAATTTCAATGGCATTTCTTCTTTCCACCCTTCCGCTAGTGGCTC
TTATTATCACCATCATCACCAGAGCGTGTGCCAAGATATTAAGCCCTGTGTTATGTGAATGGACAGAGGC
CTTGAAGGCCACTCTGCTCTCCCTCTTCTGCCTTCTTCTCCTCTCCCCTCCGAGAGGGGCAGCCAGGAAC
TGCAACGGACTCACACTGTGCACGCTGGATAGCGAATAGCAGTAAGTAGCACACCCATCACTTAGACAAA
TACCCAAGGGAGTTCTGCTCACCGATATTTGCCCGGCCCCTGGAAGAGGAAACCTTTCGAAAGCTAATAT
CCCAGAAGAGCGACAGACAGAGGAGGTGACTACATGTAAGACATATGTTACTGTGTGGAGGACATAAAAC
TTTTCAGTTCTGGGGTGGCCATTGCATTCACTAATCAGGGTCTGAAAAGGGAGGTGTGTGTG
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:GGTTATGGTGGCCTCGACAT; Reverse Primer - name:unspecified, sequence:CACACACACCTCCCTTTTCA. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:13827 same embryo
 EMAGE:13826 same embryo
 EMAGE:13824 same embryo
 EMAGE:13825 same embryo
 EurExpress:euxassay_019593 same experiment
 MGI:4824905 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS