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EMAGE:13875

Rorc RAR-related orphan receptor gamma ( MGI:104856)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:13875 EMAGE:13875 EMAGE:13875 EMAGE:13875 EMAGE:13875
"Pseudo-wholemount" of euxassay_019635. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_019635_01 euxassay_019635_02 euxassay_019635_03 euxassay_019635_04
EMAGE:13875 EMAGE:13875 EMAGE:13875 EMAGE:13875 EMAGE:13875
euxassay_019635_05 euxassay_019635_06 euxassay_019635_07 euxassay_019635_08 euxassay_019635_09
EMAGE:13875 EMAGE:13875 EMAGE:13875 EMAGE:13875 EMAGE:13875
euxassay_019635_10 euxassay_019635_11 euxassay_019635_12 euxassay_019635_13 euxassay_019635_14
EMAGE:13875 EMAGE:13875 EMAGE:13875 EMAGE:13875 EMAGE:13875
euxassay_019635_15 euxassay_019635_16 euxassay_019635_17 euxassay_019635_18 euxassay_019635_19
EMAGE:13875 EMAGE:13875 EMAGE:13875 EMAGE:13875 EMAGE:13875
euxassay_019635_20 euxassay_019635_21 euxassay_019635_22 euxassay_019635_23 euxassay_019635_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:13875Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
13875_wholemount_strong.wlz
13875_wholemount_moderate.wlz
13875_wholemount_weak.wlz
13875_wholemount_possible.wlz
13875_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:13875_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
thyroid gland
moderate moderate
regionalmoderate expression: see section 13 14 weak expression: see section 18
pituitary gland
weak weak
regionalweak expression: see section 13 14 15 16 17
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T55168
Entity Detected:Rorc, RAR-related orphan receptor gamma ( MGI:104856)
Sequence:sense strand is shown

>T55168
CTCATCGACAAGGCCTCCTAGCCAAGCTGCCACCCAAAGGAAAACTCCGGAGCCTGTGCAGCCAACATGT
GGAAAAGCTGCAGATCTTCCAGCACCTCCACCCCATCGTGGTCCAAGCCGCCTTCCCTCCACTCTATAAG
GAACTCTTCAGCACTGATGTTGAATCCCCTGAGGGGCTGTCAAAGTGATCTGGAGGAAGGACAACTTTCT
ATTTCCTTCAGCCCTCTGACCCGTCTCCCTGGACTCCCTTCACCCAGCCTTTCCCTTTCTGCACTCTATG
AAGGGTGGTATCCCTAGGAGTAAGCAAATCCTAAGACTGATTTTCTGCCCCTAGGCTTGCCTTGTAGGAC
AACAGCAGCAAGTGATGGAGAAAAGGCTTGTTATGTTTGATTTCCCATAAGTTCCACCCTGGCTTCTGGA
AGCTGTGGGGTAGATGGGATAGAGATAGGATGACCAAGTCAAATAAAAAACAGACTGACAATCAGCAGGG
ATAAATCCAGGTACCTGGGATAAGGAGAACTCAAATCTAGGCTTGAAAGCTAATAACAGTCCTTTCAATA
CCTCATTGTATTTCCCCATGGGTCCTCCTGGGGGGACATGGATCTAGCTCAGAGACTGGTGGCAAGCCCC
CAGAAGGACCTGTATATAATAAGAATATAGATTCCTGAGACTTTTCTGCCTTTCTTCTTCCTAGTTAAGA
AATGTTGTTGACCCCCTCTGCCTGTTTTCTGGGACCTAAAATGCCTGGATGTGTAAAGAATGAGGGTGGG
GTGGAGATAAGGTCCCAAGATAACTGTTTTATGGGGTTTGGGTATGAAGAAAAACATCACTGGAAAAATT
AGAATGGAAACCTCTTTGCACACTTTAAAAGTGTCAGATTCGTTAGCAGTCTAATCAGAGACACACATCC
ACACAGGTGGAGCACACAGAGGCTCTGCCCCCAGTGACACCATTCTGTAGACTTTCCCTCTGGCACACAA
T
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:CTCATCGACAAGGCCTCCTA; Reverse Primer - name:unspecified, sequence:ATTGTGTGCCAGAGGGAAAG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:MGI:4827791 same experiment
 EurExpress:euxassay_019635 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS