EMAGE mouse gene expression spatial database

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EMAGE:13961

Tgif1 TGFB-induced factor homeobox 1 ( MGI:1194497)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007804. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007804_01	euxassay_007804_02	euxassay_007804_03	euxassay_007804_04

euxassay_007804_05	euxassay_007804_06	euxassay_007804_07	euxassay_007804_08	euxassay_007804_09

euxassay_007804_10	euxassay_007804_11	euxassay_007804_12	euxassay_007804_13	euxassay_007804_14

euxassay_007804_15	euxassay_007804_16	euxassay_007804_17	euxassay_007804_18	euxassay_007804_19

euxassay_007804_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13961_wholemount_strong.wlz

13961_wholemount_moderate.wlz

13961_wholemount_weak.wlz

13961_wholemount_possible.wlz

13961_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13961_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

moderate

regional

moderate expression: see section 03 04 16 17 18 weak expression: see section 02

diencephalon lateral wall ventricular layer

weak

regional

weak expression: see section 10 11 12

telencephalon ventricular layer

moderate

regional

moderate expression: see section 04 05 07 08 09 10 12 13 14 15 16 17 18 weak expression: see section 06

midbrain ventricular layer

moderate

regional

moderate expression: see section 09 12 13 15 weak expression: see section 07 08 10 11 14

esophagus

moderate

regional

moderate expression: see section 08

hindgut

weak

regional

weak expression: see section 10

rectum

weak

regional

weak expression: see section 10

midgut

weak

regional

weak expression: see section 06 07 08 09 10 11

bladder

weak

regional

Expression in the fundus region.

renal cortex

weak

regional

weak expression: see section 05 06 07 12 13 14 15

testis

weak

regional

weak expression: see section 03 04 05 14 15

urethra of male

weak

regional

weak expression: see section 09 10

lung

moderate

regional

moderate expression: see section 02 03 05 06 09 10 11 12 13 14 15 weak expression: see section 04 07 08 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3076
Entity Detected:	Tgif1, TGFB-induced factor homeobox 1 ( MGI:1194497)
Sequence:	sense strand is shown >T3076 TGNCCTCGAGNCAGAATTCGGACGAGGTGCACTCAAACGAGCGGCAGAGATGGAGCTTCAGGCCAAACTC ACAGCTTAACCGTTTTTTCAAACAAAACAGTTCTCCAAAATACGGTCCTGATTGCCGGGGGTGATGGCAA GAGATGCATTATTTTATATATTTTTCTATTAATATTTGCACATGGGATTGCTCAGACGAAGCTTCCTGTT ACTAAGATGTCTTAAGTGGAATAGAGTCATTCCAAGAACTACAAACTAAAGCTACTGTAGAAACAAAGGG TTTTCTTTTCGAATGTTTCTTGGTAGTTTCTCATAATGTGAGACGGTTCCCAGTATCATGTGATCTTCTC CTCCAGACTCCTCTTCTTTATGTTCCAAGACTGTGCAATACTTTAGACGCCCTCGCACCTCTCTCTTCCC ATGTGGAATGGGACGCCCACCTACAGTCTAATGAGTAAACTTTCAGTTTTTTGTTTGTTTGTTTTTTTTT AGATTCAAGCAAGTATGAATCTAGTTGTTGGATACCTTTTTTCATGATGTAATAAAGTA
Notes:	The probe template was PCR amplified from IMAGE:1546796 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1546796 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13957 same embryo
	EMAGE:13959 same embryo
	EMAGE:13956 same embryo
	EMAGE:13958 same embryo
	EMAGE:13960 same embryo
	EurExpress:euxassay_007804 same experiment
	MGI:4828682 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source