EMAGE mouse gene expression spatial database

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EMAGE:14014

Cyld cylindromatosis (turban tumor syndrome) ( MGI:1921506)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001691. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001691_01	euxassay_001691_02	euxassay_001691_03	euxassay_001691_04

euxassay_001691_05	euxassay_001691_06	euxassay_001691_07	euxassay_001691_08	euxassay_001691_09

euxassay_001691_10	euxassay_001691_11	euxassay_001691_12	euxassay_001691_13	euxassay_001691_14

euxassay_001691_15	euxassay_001691_16	euxassay_001691_17	euxassay_001691_18	euxassay_001691_19

euxassay_001691_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14014_wholemount_strong.wlz

14014_wholemount_moderate.wlz

14014_wholemount_weak.wlz

14014_wholemount_possible.wlz

14014_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14014_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

foregut-midgut junction

strong

regional

strong expression: see section 09 10 11 12

pituitary gland

moderate

regional

moderate expression: see section 08 09 10 11 12 13

epidermis

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

vibrissa

strong

regional

strong expression: see section 01 moderate expression: see section 02 03 04 18 19 weak expression: see section 20

naris

strong

regional

strong expression: see section 08 09 10 12 13

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 06 07 08 09 12 13 14 15 16

esophagus

strong

regional

strong expression: see section 12

stomach

strong

regional

strong expression: see section 01 02 03 04 05 06 07

hindgut

strong

regional

strong expression: see section 10 11 12 13

rectum

strong

regional

strong expression: see section 10

midgut

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17

oral epithelium

strong

regional

strong expression: see section 18 moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17

bladder

moderate

regional

moderate expression: see section 10 11

urethra of male

strong

regional

strong expression: see section 10 11

lung

strong

spotted

strong expression: see section 02 03 04 05 06 07 08 09 10 12 13 14 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3071
Entity Detected:	Cyld, cylindromatosis (turban tumor syndrome) ( MGI:1921506)
Sequence:	sense strand is shown >T3071 TGGCCTCGAGCCAGATTCGGCACGAGGAAGGCTGTGCGCGCAGACTTCTTTGCGATGCATACATGTGCAT GTACCAGAGTCCAACCATGAGCCTGTACAAATAACCAGGTTATCCGCAGTGGGCAGAGACAGCACAAACC TTAGTGCTGTGTCTTCCTCCAGCTTGTGGTTCTGTCCGGCACCCATTGCCGGCAATGGGTGTCTCTTTTG ATGATGGCCCTTCAGCAAAGGATTCCTGTGTTTCCAAACAAATTGCTTTTGTGTTCCTGAAGTATTTAAT AAGAAGCATTTTGCACTCTAGGAAGTATGTTTGTGTTGATTTTTTAAGAAGTCTAAATGGATTTATTAAT ATCTGACACTTTAAGTTAACTGCATTGATGATACGATATTTTTGGAAGCATACAATTTTAATTGTGACGT CTAAAACCTCTTTTAGTCCATTGAGGATGTAAATAAATATTTCTTCTTATGGACCAGGGATATGAAATCA TTTTCCTTTTGTAGCTAATGATTGCCTTGAGAAAGACATAATATGATTTTATTAAGAGTCTGCCTTTACT CCAAT
Notes:	The probe template was PCR amplified from IMAGE:1546667 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1546667 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14013 same embryo
	EMAGE:14015 same embryo
	EMAGE:14012 same embryo
	EMAGE:14011 same embryo
	EMAGE:14016 same embryo
	EurExpress:euxassay_001691 same experiment
	MGI:4824156 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source