EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:14021

Rab3ip RAB3A interacting protein ( MGI:105933)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007883. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007883_01	euxassay_007883_02	euxassay_007883_03	euxassay_007883_04

euxassay_007883_05	euxassay_007883_06	euxassay_007883_07	euxassay_007883_08	euxassay_007883_09

euxassay_007883_10	euxassay_007883_11	euxassay_007883_12	euxassay_007883_13	euxassay_007883_14

euxassay_007883_15	euxassay_007883_16	euxassay_007883_17	euxassay_007883_18	euxassay_007883_19

euxassay_007883_20	euxassay_007883_21	euxassay_007883_22	euxassay_007883_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14021_wholemount_strong.wlz

14021_wholemount_moderate.wlz

14021_wholemount_weak.wlz

14021_wholemount_possible.wlz

14021_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14021_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

stomach

moderate

regional

moderate expression: see section 01 02 03 04 weak expression: see section 05 06 07

hindgut

weak

regional

weak expression: see section 13

midgut loop

weak

regional

weak expression: see section 09 10 11 12 13 14 15 16

kidney calyx

strong

regional

strong expression: see section 05 06 07 08 09 15 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1365
Entity Detected:	Rab3ip, RAB3A interacting protein ( MGI:105933)
Sequence:	sense strand is shown >T1365 CTGGATCGCAGAGAGCGCCAGCCGCGGCGGAGTGGAGCGGCGTCTGTCCGCCGCCGTCCTCCGCGTCTCC CGAGCTGGGCACGCGCACCCTGCGGCTCTGCGAGCGCTGCCGAGCGCGGGCGGCGGCCGCGGCGGATCCG GCAGCTTAGGTTGTCCTATGATGAAGCCTTTGCTATGGCTAACGACCCCTTGGAAGGCTTCCATGAAGTG AACCTTGCTTCGCCTACTTCTCCAGACCTTCTTGGTGTGTGTGACCCGGGAACCCAAGAGCAGACTACCT CCCCGAGCGTCATCTACCGGCCACACCCCTCCACGTTATGCGCTGCCCCTCTCCAGGCCAATGCCTTAGA TCTTTCTGATCTTCCTACACAACCTGTGTATTCGTCCCCCAGGCATTTCAACTGTGCAGAAGTATCTAAT ATCAGCGCTCATGCTCCGGATCCAGCATCATCGGTACCCTCTGCGGTAGCATCAGGGCTCACGAAGTTAA CCTCCAGGAAGGACAGCTGTAATGCAGAGAGGGAGTTTCTGCAGGGCGCCACCATCACCGAGGCTT
Notes:	The probe template was PCR amplified from IMAGE:2317657 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2317657 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14023 same embryo
	EMAGE:14024 same embryo
	EMAGE:14022 same embryo
	EurExpress:euxassay_007883 same experiment
	MGI:4827581 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source