EMAGE mouse gene expression spatial database

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EMAGE:14067

Lum lumican ( MGI:109347)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001718. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001718_01	euxassay_001718_02	euxassay_001718_03	euxassay_001718_04

euxassay_001718_05	euxassay_001718_06	euxassay_001718_07	euxassay_001718_08	euxassay_001718_09

euxassay_001718_10	euxassay_001718_11	euxassay_001718_12	euxassay_001718_13	euxassay_001718_14

euxassay_001718_15	euxassay_001718_16	euxassay_001718_17	euxassay_001718_18	euxassay_001718_19

euxassay_001718_20	euxassay_001718_21	euxassay_001718_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14067_wholemount_strong.wlz

14067_wholemount_moderate.wlz

14067_wholemount_weak.wlz

14067_wholemount_possible.wlz

14067_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14067_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 15 16 17 18 19 20

diaphragm

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

head mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

dermis

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

diencephalon meninges

moderate

homogeneous

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18

telencephalon meninges

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 weak expression: see section 21 22

hindbrain meninges

moderate

homogeneous

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

midbrain meninges

moderate

homogeneous

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

spinal cord meninges

moderate

homogeneous

moderate expression: see section 07 08 09 10 11 12 13 14

cochlea

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 16 17 moderate expression: see section 18 19 20

tongue muscle

weak

regional

weak expression: see section 11 12 13 14 15

stomach

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08

hindgut

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15

midgut

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16

bladder

strong

regional

strong expression: see section 13

bladder

strong

regional

Expression in the fundus region.

clavicle

moderate

homogeneous

moderate expression: see section 07 08 09 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2752
Entity Detected:	Lum, lumican ( MGI:109347)
Sequence:	sense strand is shown >T2752 AGAGTATACCAACAGTTAATGAAAATCTTGAAAATTATTACCTGGAGGTCAATGAACTTGAAAAGTTTGA TGTGAAGAGCTTCTGTAAGATCCTGGGACCACTGTCTTACTCCAAGATCAAGCATCTGCGCTTGGATGGC AATCCTCTCACTCAGAGCAGTCTGCCTCCTGACATGTATGAGTGTCTACGTGTAGCAAATGAAATCACCG TTAACTAACATCCTCTCATTCCAATACATTGAAGTATGTTCTGGAGCAGAACTTCATGGTTGGGAATGTG GGGGATGTTTTAAAGTTTTCACAATATCCTTCTCGTTGCTGGTGGTATTACTTCATGGATTTTAATTAAT GAAGGAAATGTTTTATGAACATTTACCACATTTAAATAAAAGATGAAAAGCAGGCCTTTTTCATCCTGAG AACAGAAATACAAAACCCGTTAAACTTAAGTCTTTATTTGTAAATTTAATGTTTTCTACAGCTCTATGTT CAGATGGTATGAAAGCCTTTTTACTGATTGCATGGAAATCAGCCAAGTTTTTTA
Notes:	The probe template was PCR amplified from IMAGE:1514427 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1514427 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14066 same embryo
	EMAGE:14065 same embryo
	EMAGE:14068 same embryo
	EurExpress:euxassay_001718 same experiment
	MGI:4826023 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source