EMAGE mouse gene expression spatial database

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EMAGE:14088

Dynll2 dynein light chain LC8-type 2 ( MGI:1915347)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001725. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001725_01	euxassay_001725_02	euxassay_001725_03	euxassay_001725_04

euxassay_001725_05	euxassay_001725_06	euxassay_001725_07	euxassay_001725_08	euxassay_001725_09

euxassay_001725_10	euxassay_001725_11	euxassay_001725_12	euxassay_001725_13	euxassay_001725_14

euxassay_001725_15	euxassay_001725_16	euxassay_001725_17	euxassay_001725_18	euxassay_001725_19

euxassay_001725_20	euxassay_001725_21	euxassay_001725_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14088_wholemount_strong.wlz

14088_wholemount_moderate.wlz

14088_wholemount_weak.wlz

14088_wholemount_possible.wlz

14088_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14088_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

regional

weak expression: see section 11 12 13 14

diencephalon

weak

homogeneous

weak expression: see section 08 09 10 11 12 13 14 15 16 17

telencephalon mantle layer

moderate

homogeneous

moderate expression: see section 05 06 07 16 17 18 19

telencephalon marginal layer

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

olfactory cortex marginal layer

moderate

homogeneous

moderate expression: see section 08 09 10 12 13 14

hindbrain

weak

homogeneous

weak expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

midbrain

weak

homogeneous

weak expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

midbrain mantle layer

moderate

homogeneous

moderate expression: see section 19

midbrain marginal layer

moderate

homogeneous

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 07 17 18

trigeminal v ganglion

weak

regional

weak expression: see section 04 05 06 07 08 15 16 17 18 19

vagus x ganglion

weak

regional

weak expression: see section 08 16

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 07 08 16 17 18

spinal cord

weak

homogeneous

weak expression: see section 11 12 13 14 15 16

dorsal root ganglion

weak

regional

weak expression: see section 08 09 10 15 16 17

not examined

regional

not examined expression: see section 01 02 20 21

neural retina

moderate

regional

moderate expression: see section 01 02 weak expression: see section 03 20 21

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 10 12 13 14 15 weak expression: see section 06 07

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4114
Entity Detected:	Dynll2, dynein light chain LC8-type 2 ( MGI:1915347)
Sequence:	sense strand is shown >T4114 CGGAGCGGACGGCGAGGTATCAGCCGAGACAGGCGGTCGGCCGGGTGGCACGAGGCGGAGCGCGGGCGTC CGGGGAAGCTCGGAGGGCGACCAGCAGTGCGAGCGCTCGGCCCCGGGGCTGCGGGAGCAGGGGGATACCG CGGCGGCAGCGGCTGCTGCTGCAGGACGAGCCCAGGGGACACCGCCCCTGCTCGCGCTTTGCTTCAGCCC AGCGCTGCTCCCCAGGGGCCTGTGCGGACTCGCCTGCGTGACGTGTCACGATGTCTGACCGGAAGGCAGT GATCAAGAACGCAGACATGTCTGAGGACATGCAACAGGATGCCGTTGACTGTGCCACACAGGCCATGGAG AAGTACAACATAGAGAAGGACATTGCTGCCTATATCAAGAAGGAATTTGACAAGAAATATAACCCTACCT GGCATTGTATCGTGGGCCGGAATTTTGGCAGCTACGTCACACACGAGACAAAGCACTTCATCTATTTTTA CTTGGGTCAAGTTGCAATCCTCCTCTTCAAGTCGGGCTAGGTGGCCGAGGCGAAGGTGTCCGTGGCGGCG GCAGCGATGGCAAGCAGGCGGCGTTGCTGGGACTGTTGCACTGGGGCCAGCATCAGGAAGTCCTCTCCAA TGGCTGTGCTACTGCATGGACTGTGTACTCGATTTCATGTGTGTGTCGCAGTAAACAAAAACCAAACTCC TTTCTGTTTAGTTGCCTGGGGAAGAAGGCTGCTTTGCGTTTGTTTTTAAGACGTTAACATTATTTTTTTG GTTGTACTGCACTAGGAAATCTCTCCCACCCCTCCTTTTCTCTTCTCTCCCTAGATAAAAATAAAAGGCC CTCCTCGAAGCCCTAAGGACTAGGAGGGCCGAGGAATAGAAATAGGTCTGAGGTGGAAGTTAAGCTGCAG GTGCCTCTTGGGTGGTGGGTGCTGCTGTAGGAGGCCGGGGAGGCTGTGAGGACAGGGTTAGGATTGGCAA GGAGAGGGGCAGGAAGGTGGGGGGGGGGTGGCTCTAGTACCAGGGAGAATATTCCACAGAACTGTGACTA TGGCTTGGGGCAGGGGTGGGTGTGCAGTCTCTAAGGGGCCCTAAGGTGTGTTTGTGGTGTGGGAATGGGG AGCAGACTCGTCTTGCCGTCTGTCAGGATTTTAAGTAAGGGCTGTGTCTTTGTGGGTCATCTTCTCATAT TCTTCCAGCCAGAAGTCGTGTTGGGAGGATGCTGGGAGTAGGGTTAGCCTGACTTCCCCTTTACATTCTG TCTGCTCCCTGCTTAGCCCTCGTTTTTTCTCATTCCTTCGTTCTCTTAGAGCAGCCCCTATTGTTGGCTG GCAAGGGAATTTCTGGTGACTGTAGTTCCTTAGTTAGGTCTTAGCAATCAAACCAAATCAATGCCCGTCA GTTCTTCTTTGTGTACGTTTGTGTTTGTCTGTGTGCGTGTGTGGGGGTGGGTTTGGGGTAGAAGGGTGGG AAGGGGCAGGATAAGTGGAATCTCTAGGGTGTATGGGAAGTAGAGGGCAGTTAGTTCTAAGTGGGCTTTT ATGTTCCCTGTTGTGAGGGTGAGAACAGGTTGGTATCAAACCATCCCCCTCCCCTTAGTTCATCTTGCTT GGACACTGCTTTGCCTTGTGGCTGTCTGTTCCCTGGGAAAGGTTTTGGAAGGTCACCCAGAACAGGATGG CCATACTAACATCTTTCCTGGAGTTGGATCTGGGACCAGGTGACTGGTCTCTAGTGGGAAGTGAGAATGG AAGTGATGGGGTGGAGAGCTCCTTGGAACAGGTCTGAAGTATAGCAATGACCTCTGGGAGGCAGGAGGTG GAGAATGGAAGTGGGGTGGAGGGCTCCTGGAACAGGTCTGAAGTATAGCAATGACCTCTGGGAGGCAGGA GGTGGAGAAGGCTGTGATTGGGAGGAGATTCCAGAGAAGAGTGCAAGGGAAACCTTGTCTGTCGGTGACA TGATTGGGGTGGGTGGGGGTTGTCATTGCCAATTGAGCTGCTGATTCTTATGAATCACATTCAGAACTCG CATAGGGTTGACACTGTAAGGGGTGGGGAAATCTAGCTATTTTTTTTTCCCCAGCCCATTCTTGGGGGTC GGGGGGAGGCAGGAGAATGCCCCTTCCTCCTAAGCCCTTCCTGTGTGTTGAGCTTTTGTTTTTCTTCAGT GCTGGCAGGGGAGGGTGGGAACTGGTGGGGACTGAGTTCCCTGTTCAAACCGTTATTGGGTACAGGATTG AATGTTTGATTTTAGGTTTTATTTTTCTTATGAATGTCCAGATCTGTGTTTCCCTGCCCTCCCAGACTCT GGCCAGTTGGAAGTGTCTCGTTTTTGTTCATCTCTCATTTCTGGTGCAGGGACTGAGACCCTGCCACATC TATGCTCTGCATCCACGCCTCTTTTGGACATTAAAGGTCGATTGATGCACTTCTGAAAAAAAAAAAAAAA AAAA
Notes:	The probe template was PCR amplified from IMAGE:4459858 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4459858 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14089 same embryo
	EMAGE:14085 same embryo
	EMAGE:14086 same embryo
	EMAGE:14087 same embryo
	EurExpress:euxassay_001725 same experiment
	MGI:4824431 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source