EMAGE mouse gene expression spatial database

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EMAGE:14291

Elmo3 engulfment and cell motility 3, ced-12 homolog (C. elegans) ( MGI:2679007)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004103. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004103_01	euxassay_004103_02	euxassay_004103_03	euxassay_004103_04

euxassay_004103_05	euxassay_004103_06	euxassay_004103_07	euxassay_004103_08	euxassay_004103_09

euxassay_004103_10	euxassay_004103_11	euxassay_004103_12	euxassay_004103_13	euxassay_004103_14

euxassay_004103_15	euxassay_004103_16	euxassay_004103_17	euxassay_004103_18	euxassay_004103_19

euxassay_004103_20	euxassay_004103_21	euxassay_004103_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14291_wholemount_strong.wlz

14291_wholemount_moderate.wlz

14291_wholemount_weak.wlz

14291_wholemount_possible.wlz

14291_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14291_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 01 02 03 04 05 06 15 16 17 18 19 20 21

humerus

weak

regional

weak expression: see section 03 21 22

femur

weak

regional

weak expression: see section 03 05 19 20 21

thymus primordium

moderate

regional

moderate expression: see section 09 10 11 12 13

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 10 11 12 13 16 17 18 19

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 14 15 17 18

pharynx epithelium

moderate

regional

moderate expression: see section 10 11 12 13

not examined

regional

not examined expression: see section 14

tongue epithelium

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17 18

stomach

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08

rectum

moderate

regional

moderate expression: see section 12 13

midgut

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17

meckel's cartilage

weak

regional

weak expression: see section 11 12 13 14 15 16 17 18 19

oral epithelium

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19

liver

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

lung

weak

regional

weak expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

nucleus pulposus

moderate

regional

moderate expression: see section 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5584
Entity Detected:	Elmo3, engulfment and cell motility 3, ced-12 homolog (C. elegans) ( MGI:2679007)
Sequence:	sense strand is shown >T5584 GGCCTCGGAGCCCGATCAGGAGTGGGAACCTGGGCTGTGAGTGCAGGCTACAAGTCCTAGACCCTAGACC GGGTGCACTCTCGGCGGGAGGTGGCACATTAGTGCCAAGTGCCAGCGGCCACCGCGGCTGGACTATGGCG CCCCCGCGGAATGTGGTGAAGATTGCTGTTCAGATGTCTGACGCCATCCCACAGCTTATCCAGCTAGACC AGGCAAAGCCCCTGGCCACTGTACTGAAGGAGGTGTGTGACGCGTGGAGCCTGACTCATCCGGAGCACTA TGCCCTGCAGTTTGCTGATGGGCACAGGAAATACATCACAGAGAATAACCGCTTGGAGATCAAGAATGGA AGCATCCTGTGTCTCAGCACTGCCCCAGATCTGAAGGCCCAGCAGCTACTGGGTAGACTGCAGAATACAA GTCGTGAAGGGTGCTGTGAAGTCCTGAGAAACCTGGTCCTGCTGGCCTCAGACATGACCTTTGCCCAGGA GGTCATCAGTCGTGATGGGCTTCAGAAACTAAGCACCATCATTGAAAATGGGGATGACCTAGGGGAGATG CTGGCCCTTGGTCTAAGGGCTTTCTTGGAGCTCATGGAACACGGTGTAGTGTCCTGGGAGACACTCAGCA TCTCCTTTGTCAGGAAGGTGATATCTTATGTGAACATGAACCTGATGGATGCCTCTGTCCAGCCCCTGGC TCTGAGACTGCTGGAGAGTGTGACCTTGAGCAGTCCTGCCCTGGGCCAGCTGGTGAAGAGTGAGGTGCCA CTGGATAGGCTGCTGGTGCACCTGCAAGTAATGAACCACCAGCTGCAAACCAAGGCCATGGCTCTGTTGA CGGCCCTGCTGCAGGGAGCCAGCCCCACTGAACGCAAGGAAATGCTTGACCACCTATGGAAGAAGAATCT TCGCCAGTTCATCTACAAGAACATCATCCACAGTGCAACACCCATGGGTGACGAGATGGCTCATCACTTG TATGTGCTGCAGGCTCTTACCCTAGGGCTGCTGGAGCCACGGATGCGGACACCACTTGATCCCTACAGCC AGGAACAGCGGGACCAGCTGCAGGCTCTGCGCCAGGCAGCCTTTGAACCAGAGGGGGAGTCCTTGGGCAC AGGGCTCAGTGCAGACCGCCGCCGGTCTCTCTGTGTCCGAGAGTTCCGCAAGTTAGGCTTCTCTAACAGC AACCCAGCCCAGGACCTGGAGCGTGTGCCCCCCGGCCTGCTGGCCCTGGACAATATGCTCTACTTCTCCA GACATGCACCAAGTGCATACAGTCGGTTTGTGTTGGAGAACAGTAGCCGAGAGGACAAGCATGAATGCCC CTTTGCCCGGAGCAGCATCCAGCTGACAGCGTTGCTGTGTGAGCTGCTCCGTGTTGGGGAGCCTTGCTCC GAGACAGCCCAGGACTTCTCGCCCATGTTCTTCAGCCAAGACCACAGTTTCCATGAGCTCTTCTGTGTGG CTATCCAGCTACTGAATAAGACCTGGAAGGAGATGCGGGCAACACAGGAAGATTTTGACAAGGTGATGCA AGTGGTTCGGGAGCAGCTGGCCCGTACCCTGGCTCTGAAGCCCACCTCCCTGGAGCTCTTTCGAACCAAA GTGAATGCCCTCACCTATGGGGAAGTGTTGAGGCTGCGGCAGACAGAGCGGCTGCACCAGGAGGGCACGC TGGCCCCTCCTATACTGGAATTGCGAGAGAAACTGAAGCCAGAGCTCATGGGCTTAATCCGCCAGCAGCG TTTGCTCCGACTCTGTGAAGGAATGCTCTTCCGCAAGATCAGCAGCCGGAGACGACAGGACAAGCTATGG TTTTGCTGTTTATCCCCCAACCACAAAGTACTGCAGTATGGGGATGTGGAGGAGGGTGCCAAGCCCCCTA CCCTAGAAAGCCTACCTGAACAGCTCCCTGTGGCAGACATCAGGGCACTCCTAATGGGCAAGGACTGCCC CCATGTCCGGGAGAAGGGCTCTGGGAAGCAGAACAAGGACCTGTATGAGCTGGCTTTCTCCATCAGCTAT GACCACGGGGAGGAAGAAGCATACCTCAACTTCATTGCCCCCTCCAAACGGGATTTCTACCTGTGGACAG ACGGGCTAAGTGCCCTGCTGGGCAGTACCATGGGCAGTGAGCTGACTCGGCTAGACCTGGAGCAGCTGCT CACTATGGAGACCAAGTTGCGGTTGTTGGAACTGGAGAACGTGCCCATTCCTGAGCAGCCTCCCCCAGTA CCCCCACCCCCTACCAACTTTAACTTCTGCTATGACTACAGCATCACTGAGCCGTGACTCAAGTTAGGCC ACAGTTGATACTGCTATAGCAGCCACGAAATCACAGACAACCTGACTAGGAGAGCCTCTAACAGAAATAA AGTTAGCCTGGCTTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6485393 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6485393 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14290 same embryo
	EMAGE:14288 same embryo
	EMAGE:14287 same embryo
	EMAGE:14289 same embryo
	EMAGE:14292 same embryo
	EurExpress:euxassay_004103 same experiment
	MGI:4824527 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source