EMAGE mouse gene expression spatial database

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EMAGE:14302

A930038C07Rik RIKEN cDNA A930038C07 gene ( MGI:1915419)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004095. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004095_01	euxassay_004095_02	euxassay_004095_03	euxassay_004095_04

euxassay_004095_05	euxassay_004095_06	euxassay_004095_07	euxassay_004095_08	euxassay_004095_09

euxassay_004095_10	euxassay_004095_11	euxassay_004095_12	euxassay_004095_13	euxassay_004095_14

euxassay_004095_15	euxassay_004095_16	euxassay_004095_17	euxassay_004095_18	euxassay_004095_19

euxassay_004095_20	euxassay_004095_21	euxassay_004095_22	euxassay_004095_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14302_wholemount_strong.wlz

14302_wholemount_moderate.wlz

14302_wholemount_weak.wlz

14302_wholemount_possible.wlz

14302_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14302_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

cervical intervertebral disc

strong

regional

strong expression: see section 09 10 11 12

lumbar intervertebral disc

strong

regional

strong expression: see section 09 10 11 12

tail vertebral pre-cartilage condensation

strong

regional

strong expression: see section 13 14

thoracic intervertebral disc

strong

regional

strong expression: see section 09 10 11

hand mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04

foot mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 06 07 08 20

body-wall mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 14 15 16 17 18 19

vibrissa

moderate

regional

moderate expression: see section 04 05 06 19 weak expression: see section 20

hypothalamus ventricular layer

strong

regional

strong expression: see section 12 13

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 11 12 14

thalamus mantle layer

weak

regional

weak expression: see section 08 09 10 15 16 17 18

medulla oblongata basal plate mantle layer

strong

single cell

strong expression: see section 11 12

medulla oblongata basal plate marginal layer

strong

single cell

strong expression: see section 10 13 14

medulla oblongata basal plate ventricular layer

strong

single cell

strong expression: see section 09 13 14

pons ventricular layer

strong

regional

strong expression: see section 07 08 15 16 17 weak expression: see section 06 18

tongue epithelium

strong

regional

strong expression: see section 09 10 11 12 13 14

lower jaw molar

moderate

regional

moderate expression: see section 06 07 17 18

upper jaw molar

moderate

regional

moderate expression: see section 05 06 07 08 16 17 18

left lung

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11

right lung

strong

regional

strong expression: see section 12 13 14 15 16 17 18 19 20

sacral vertebral cartilage condensation

strong

regional

strong expression: see section 10 11 12

tail mesenchyme

moderate

regional

moderate expression: see section 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5602
Entity Detected:	A930038C07Rik, RIKEN cDNA A930038C07 gene ( MGI:1915419)
Sequence:	sense strand is shown >T5602 AGCAGAGCTTAGCGAGCCGCGTATGAGCCGCCGCCTCTGTGGCTGGTGCACGGGGCTCTGTCTCTGCAGC CCGCGCCGGCGTTTAGCGAGCCAGTCGTCCGGCAGTGCCAGCTCCACGGGCGCGTTCAGTATCCTCCCGC CACCCAGCGCCCGCACTGACCAGCGCTGTCCTGAGACCCCCGGGCCGGCGCCGCCGTCTGCGCCACTTCG CAGCAACTTCTGCGGCTGCGTGTCGCCGGCCTTCTGCTTGTGTTGGAAGTTTGCAGCGCCCGCCACAAAG TTGGGACACTTGGGCATTACATTTGCCCTAGCGTGCACGTCCCCTCGGTTCGTGGATCCTGGTATTTGAC ATCTTGGTAGGATCCCCGGACCTGGGCTTTTTCCGCACCACACACCCCCCGGGAAGAAGAAAAAAAAAAC CAACCAAACTTTCTTCTTCCCACTAGAAAAAACAAATTCTTTAAGGATGGAGCTGTTCTACTGGTGTTTG CTGTGCCTCCTGTTACCACTCACTTCAAGGACCCAGAAGCTGCCCACCCGAGATGAGGAACTTTTTCAGA TGCAGATCCGGGATAAGGAATTTTTTCACGATTCATCTGTGATTCCAGATGGAGCTGAAGTCAGCAGTTA TCTCTTTAGAGATACACCTAGAAGGTATTTCTTCATGGTTGAGGAAGATAACACCCCACTGTCAGTCACA GTGACACCTTGCGATGCACCTTTGGAGTGGAAGCTTAGCCTTCAGGAGCTGCATGAGGGGTCCAGTGCAG ATGGATCAGGTGACCCAGAACTACTTGACCAGCAGAAGCAGCAGATGACTGATGTAGAGGGCACAGAACT ATTCTCCTACAAGGGCAATGATGTAGAGTATTTTCTGTCTTCAAGTTCCCCATCTGGTTTGTATCAGTTG GAGCTTCTTTCAACAGAGAAAGACACACATTTCAAAGTATATGCCACCACCACACCAGAATCTGACCAGC CATACCCTGAATTACCATATGACCCCAGAGTTGATGTGACCTCCTTTGGACGTACCACAGTCACTTTGGC CTGGAAGCCAAGCCCTACAGCCTCTATATTGAAACAACCCATAGAGTACTGTGTGGTCATCAACAAGGAG CACAATTTCAAAAGCCTCTGTGCAGCAGAAACAAAGATGAATGCAGATGATGCCTTCATGGTGGCGCCCA AACCTGGTCTAGACTTTAACCCCTTTGACTTTGCCCATTTTGGATTTCCAACAGATAATTTGGGTAAAGA TCGAAGCCTCCTGGCAAAGCCTTCTCCCAAAGTGGGGCGCCATGTCTACTGGAGGCCCAAGGTTGACATT CAAAAAATTTGCATAGGAAATAAAAACATTTTCACAGTCTCTGACCTGAAGCCCGATACCCAATACTACT TTGATGTCTTCATGGTCAATACCAACACCAACATGAGTACAGCTTATGTGGGTGCCTTTGTCAGGACCAA GGAGGAGGCAAAACAGAAGACAGTGGAACTCAAAGACGGGAGGGTCACAGATGTGTTTGTTAAAAGGAAG GGGAAAAAGTTTCTACGGTTTGCTCCAGTCTCCTCTCACCAAAAGGTCACGTTCTTTATTCATTCTTGTA TGGATGCCGTTCAAGTCCAAGTGAGAAGAGATGGGAGGCTGCTTCTGTCACAGAATGTGGAAGGCATTCG GCAGTTTCAGTTAAGAGGAAAACCCAAAGGAAAATACCTCATTCGACTGAAAGGCAACAGGAAAGGAGCA TCAAAGCTGAAAATACTGGCGACCACCAGGCCCAGTAAGCATGCATTCCCCTCTCTTCCCGAAGACACAA GAATCAAAGCTTTTGACAAGCTACGTACCTGCTCTTCAGTTACGGTGGCATGGCTTGGCACCCAAGAAAG GAGAAAGTTTTGTATCTACAGAAAGGAAGTGGATGGAAACTACAGTGAAGACCAGAAGAGAAGAGAGCAA AACCAGTGCCTAGGACCAGACACCAGAAAGAAATCAGAGAAGGTCCTTTGCAAATACTTCCACAGCCAAA ACCTGCAGAAAGCAGTGACTACAGAGACAATCAGAGATCTGCAGCCTGGGAAGTCTTACCTGCTGGATGT TTACGTCGTAGGACATGGGGGACACTCTGTGAAGTATCAAAGTAAAATTGTGAAAACAAGGAAGGTCTGT TAGTCACCTTGAGTGAAGATCAGGAGAACTCCAGGAGAGACATGGAGTCACACTGCTTGTAACTGACTAC TCTCGTGACAAACAAAAGTTGTACTTGAAAGAAAGGGTAACAACATGTGTACATTGATGCTTGCCTAATG TAGCATGGAGACTTGTACTCACGCACACCTGTGGTACTTAGGGTCCATCTGTCTAATGCTGGTTAATGTC AAAAGAAGAGGGTATCTCAATGGATGCCACCCCTTGCTTTGACCATTGCATGAACTGCTTCTAAATTATT TTATTACCTAAAATTTTAAAATATGCCATTCGTTGCACACATTCACAAATGCAAATAATTCCTCTCTATA GATGTTAGGGGAAGTTTATATATAAATATATAAATATAAATTATTTTATAAAGTCTGTTTTAATTGTTGG TGTTTCTATTATTGTAAGCTATTAAATTCTTTCTCTGTTAAAGTATAGATCTAATCTAAGATCGTATCAC CCTCTAAGCAGTAATATTGTTGTAAATTCTTATTTGTTCAGTAAAATGTTTAAATGCTGTCTGTATGTAT CTCATGTAAAAAGTTGATATACATTATATTCATGTATATAAAATTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6489681 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6489681 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14303 same embryo
	EurExpress:euxassay_004095 same experiment
	MGI:4822847 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source