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EMAGE:14318

Slc38a10 solute carrier family 38, member 10 ( MGI:1919305)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14318 EMAGE:14318 EMAGE:14318 EMAGE:14318 EMAGE:14318
"Pseudo-wholemount" of euxassay_004151. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_004151_01 euxassay_004151_02 euxassay_004151_03 euxassay_004151_04
EMAGE:14318 EMAGE:14318 EMAGE:14318 EMAGE:14318 EMAGE:14318
euxassay_004151_05 euxassay_004151_06 euxassay_004151_07 euxassay_004151_08 euxassay_004151_09
EMAGE:14318 EMAGE:14318 EMAGE:14318 EMAGE:14318 EMAGE:14318
euxassay_004151_10 euxassay_004151_11 euxassay_004151_12 euxassay_004151_13 euxassay_004151_14
EMAGE:14318 EMAGE:14318 EMAGE:14318 EMAGE:14318 EMAGE:14318
euxassay_004151_15 euxassay_004151_16 euxassay_004151_17 euxassay_004151_18 euxassay_004151_19
EMAGE:14318 EMAGE:14318 EMAGE:14318
euxassay_004151_20 euxassay_004151_21 euxassay_004151_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14318Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14318_wholemount_strong.wlz
14318_wholemount_moderate.wlz
14318_wholemount_weak.wlz
14318_wholemount_possible.wlz
14318_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14318_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
rib
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 18 19 20 21 22
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 08 15 16 17
mandible
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 10 11 15 16 17 18 19 20 21 22
palatal shelf
moderate moderate
regionalmoderate expression: see section 05 06 07 08 09 10 11 12 15 16 17 18 19 20
orbito-sphenoid
moderate moderate
regionalmoderate expression: see section 02 03 04 05 21 22
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T5632
Entity Detected:Slc38a10, solute carrier family 38, member 10 ( MGI:1919305)
Sequence:sense strand is shown

>T5632
CTGAGGTGTTGCTAGCGTTGCCTGCGGCGCTTCCGGGTTCCCGCTTCACTCTGGGGAGGCGGAGGCGGGA
ACCTGGAACCATGGCTATGAGGCTGCTGCGGAGCCTGGCAGGGTCTGGAGGCTGACCTCGCGACGCCTGT
CCGCGGCCCCCCCTGCTTCAGGTTTAGGGTTATCTAGGGTCGCTCTGTCCTGGTCCCTAGACCCTCAAAA
TGACGGCCGCCTCCACCTCCAAGTGGGGGCTGATCACGAACGTGGTGAACAGCATCGTGGGCGTCAGCGT
GCTCACCATGCCTTTCTGCTTCAAGCAGTGTGGCATTGTCCTGGGGGCCCTGCTCCTGGTCTTCTGCTCC
TGGATGACACACCAGTCTTGCATGTTCTTGGTGAAGTCGGCCAGCCTGAGCAAGAGGAGGACCTATGCTG
GCCTGGCGTTCCACGCCTACGGCAAGGCCGGGAAGATGCTGGTGGAGACCAGCATGATTGGGCTGATGCT
GGGATCCTGCATTACCTTCTATGTCGTGATCGGTGACTTGGGGTCCAACTTCTTTGCTCCGCTGCTTGGA
TTACAGGTGACCAGGACTGTCCGTGTGTTCCTGCTCTTCGCAGTGTCCCTGTTCATTGTGCTCCCGCTCA
GCCTGCAGAGAAACATGATGGCCTCTATCCAGTCCTTCAGTGCCATGGCGCTCCTCTTCTACACCGTCTT
CATGTTCGTGATTGTGCTGTCCTCCCTTAAACATGGCCTCTTTAGTGGGCAGTGGCTGCGACAGGTCAGC
TACATTCGCTGGGAAGGTGTTTTCCGCTGTGTCCCCATCTTTGGCATGTCCTTTGCCTGTCAGTCCCAGG
TCCTGCCCACCTATGACAGCCTGGACGAGCCATCAGTGAAGACCATGAGCTCCATCTTTGCCTCCTCCCT
CAACGTGGTCACCGCCTTCTATGTCATGGTGGGGTTTTTTGGTTACGTCAGCTTCACTGATGCCACCACA
GGCAATGTGCTGATCCACTTCCCCTCCAACCCGGTGACAGAGATGATCCGAGTGGGCTTCGTGATGTCTG
TGGCTGTGGGCTTCCCCATGATGATTCTGCCGTGCAGGCAGGCCTTGAACACACTGCTGTTTGAGCAGCA
GCAAAAAGATGGAACCTTTGCTGCAGGAGGCTACATGCCCCCACTCCGGTTTAAAGTCCTCACCCTCTCG
GTGGTGTTTGGAACCATGGTTGGTGGGGTCATGATCCCCAATGTGGAAACCATCCTTGGCTTCACAGGAG
CAACGATGGGGAGCCTCATCTGCTTTATCTGCCCGGCTCTGATCTATAAGAAAGCCCACAAGAATGCCCC
CTCAGCCCAGGTGGTGCTCTGGGTCGGCCTGGGCATCCTCGTGGTCAGCACACTCACCACCCTCTCTGTG
ACCGAAGAAGCTCCTCTGGACTTGACGCAAGAAGCTCGCAGCGGCCACCGAGGAGATGCTGAGGGCGCAA
TGAAGGTGGAGGCAGCTCGGCTATCAGTCCAGGATCCCGTTGTAGTTGTTGCTGAGGATAGCCAAGAGAA
GCTAAAGCCAGCAGAGGACAAAGAGGTACTGGAGCAGGCCCAGATCAAGGGTCCTGTAGATGTGCCTGGC
GGGGAAGCTCCCAAGGAGAAGCAGGAAGCCGCACAGCTGGATCGCCCCGGCCAAGGTATTGCTGTCCCTA
TGGGTGAGGCCCATCGCCATGAGCCTCCCATCCCTCATGATAAAGTGGTGGTGGATGAAGGCCAGGACCA
AGAAGGGCCAGAGGAGAAAAAGCCACCTCCCAGGCTCCCAGATGAAGGAGACCCTGCAGGCAGGGGTCAA
GGGGCACCACCTCTGCCTGAGTCAGAGAAGGAGAAGCAGGAACCTGAGAGAGGAGGGGAAGGAAAGAGAC
CCGGGCAAGTCCTGGCAGTAGGAGAAACTGAACATCCTCAGAAGGTTCCAGAAGCAAATGGCCAGCCACC
TGTGCAGCCCAGGAAGGAAGATTCCAGGCCAGGAAATAGGGATCCGCAGCCAGCTGCCCAGGCCAGGGAC
TCTGTGGAGCTGAAGGCTCTGGCAGCAGATGACGGCAGGGAGCCTGCACAGAAGGCTGGAGGCGCCCTGT
GGAAGCCCGTGGAGAGTGCTGCTGAGAGCGATGCTGGTGGGAAGGCGGGCCTCCCTGTGCAAAGGCCGGA
GGCTGCAGAGCAGAGGGAGAAGAAGGAGGCTGAGCAACAGGGTGGAGACCAGGCGGGGAGCAAGCTGGAA
GCTGAAATTAAAAAGCTAGTAGCAGAAGCTGGGAGGGCAGAGATGCTGGACCATGCCGTGCTGCTACAGG
TGATCCAAGAGCAGCAGGTGCAGCAGAAGCGCCTCCTGGACCAGCAGGAAAAGCTGCTCGCAGTGATTGA
GGAGCAGCACAAGGAGATCCGGCAGCAGCGACAGGAGGGCGAGGAAGACAAGCCTAAGCCTGACGTGCAG
CCAGAGCCTGGGGTGGCTGTACTCCGAGGACAGGAGGAGGAGGCTGAGCACGCTGGGGAGACACTGGGAG
ATGACCCTTCACAGCCTTTGCAACCCGTGCTTGGAGCTCCTAGGGGTCGCCCCGCTCCATCCCAAGACAT
GGGCCAGCACCTCCCAGGGGAAGTCAAGGTGCTGCCAGGCAGAGACCTTGCTGACCTTCCTGCTGGTGGC
TCTGAAACAGAGCCCCAGGGGGCCCCGATTGATCTGAGAGAAGACCCGAAAGCTGCCATCAAGGCGGCTG
GAGCTGGGAAGGAGCTGGTCCCAGGGGACTTGGAAGCAGTGCACAAGGCAGCCCCTCCTGAGGTACCCAA
GAGTCCAGAGAAGCAGGTTGCCAAGGCAGTTGCCGGGCAGCGCCAAGATGTCTTTGGTGAAGGCTCCGAA
GAAAGGAAAGAAACTGGAAAGGAAGCAATGGCCCCTGGTGCTGATACTCAGAAAGAGGCTGTCCAGCCCT
TGGTAGGAGCAGAAGCTAAGGACACAAAATCCAGGCAGTCGGGACCCACCAAGGCCCCAGTTCAGACCCA
AGCCAAGTTTCACCCAGAACCCCAGGCCATCTTTGACACAGGTCAGGGTTCTCACCCAGAGGTGAGAAGT
GAGGCCCCCCGAGCGGTTCACATACCTCCTGAGGAGCAGCACAAAGGAAAAGGGGGTGCCGCCATCCAGG
AGGCAAAGCAGAGACCAGATCCTAACTCTGGGCCCAAACTAGCTGTGCCTGCGGGTCAGAAGCCAGAGAA
TGCCAAACCCAACCGAGACCTAAAAGTGCAGGCTGGCTCTGACCTGCGGAGGAGACGGCGGGATCTGGCC
TCTCATCCAGAGCAGGAGCTGGCTCCAAAGGATGGCGTCATCATTAGCTTTAACTCCCTCCCTAATGTTC
AGGTGAACGACCTCCGCAGTGCTCTGGACACCCAGCTCCGCCAGGCTGCAGGGGCTGCGTTGCAAGTGGT
ACACAGCCGACAGATTAAACAGTTGTCTGGAGATCTGGAGGAAGCCTGAGGGCCTGGTCAGGGAAAGGCT
GCCACTAGACATTGGACGGCAGCTGGTGTTCTCTGGTGCCACCCTGATTGGAGGCCACGTTCTTATGTTC
CCAGGGACTGGTATCTAAGTCCCTCATGATGAGTTGTGAAAGTCTAACCTGCCACCCCACCCCAGACATC
ACTTGCTCCAAGGCTACACCAAAGCACTCACTAGCCCGATTATGGTTTGGGTTTTGAGGCAGCCCAGGAA
AGACAGTGCTAAGGTTCAGCTCCGACGCTTGTCTGTTCAGACTCACGGTCTGGCCAGAAGCAGTGCTGAC
AGCATGGCAGGCAACTTTGAAGTTGCCAGTCTTCCTCTAACTTGCAACCTGTGACAGCCAGATTTCACTG
ACTCCATAAGGTGGGAAACCTACAACAGGCATGTCTGTCTGCCCTGGGGTGGCCGGGGGGTGGGGGGTAT
GACTTGACTTGGGCTGGGGAAAGAGGCACACAGCTCCCCTGGTTTTTATCACCCACATTCTTCTGAAGGC
TGATTATGAAACAAAAAAATAAAACGCCACTGACAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:6493546 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6493546 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14316 same embryo
 EMAGE:14315 same embryo
 EMAGE:14317 same embryo
 EurExpress:euxassay_004151 same experiment
 MGI:4828212 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS