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EMAGE:14321

Fam101a family with sequence similarity 101, member A ( MGI:1920371)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14321 EMAGE:14321 EMAGE:14321 EMAGE:14321 EMAGE:14321
"Pseudo-wholemount" of euxassay_004115. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_004115_01 euxassay_004115_02 euxassay_004115_03 euxassay_004115_04
EMAGE:14321 EMAGE:14321 EMAGE:14321 EMAGE:14321 EMAGE:14321
euxassay_004115_05 euxassay_004115_06 euxassay_004115_07 euxassay_004115_08 euxassay_004115_09
EMAGE:14321 EMAGE:14321 EMAGE:14321 EMAGE:14321 EMAGE:14321
euxassay_004115_10 euxassay_004115_11 euxassay_004115_12 euxassay_004115_13 euxassay_004115_14
EMAGE:14321 EMAGE:14321 EMAGE:14321 EMAGE:14321 EMAGE:14321
euxassay_004115_15 euxassay_004115_16 euxassay_004115_17 euxassay_004115_18 euxassay_004115_19
EMAGE:14321 EMAGE:14321
euxassay_004115_20 euxassay_004115_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14321Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14321_wholemount_strong.wlz
14321_wholemount_moderate.wlz
14321_wholemount_weak.wlz
14321_wholemount_possible.wlz
14321_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14321_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
rib
moderate moderate
regionalmoderate expression: see section 01 03 04 05 06 07 08 09 10 12 13 14 15 16 17 18 21 weak expression: see section 19
radius
moderate moderate
regionalmoderate expression: see section 01
ulna
moderate moderate
regionalmoderate expression: see section 01
humerus
moderate moderate
regionalmoderate expression: see section 03 04
hand
strong strong
regionalstrong expression: see section 06 07 moderate expression: see section 02 03 04 05 21
foot
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 21
fibula
moderate moderate
regionalmoderate expression: see section 02 03
tibia
moderate moderate
regionalmoderate expression: see section 02 03
femur
moderate moderate
regionalmoderate expression: see section 02 03 15 16 21 weak expression: see section 04 05 06 07 17 18 19 20
otic capsule
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 14 15 16 17 18 19
nasal septum
strong strong
regionalstrong expression: see section 09 11 12 13 moderate expression: see section 10
viscerocranium
strong strong
regionalExpression in the turbinate bone.
meckel's cartilage
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14 15
palatal shelf
strong strong
regionalstrong expression: see section 11 12 15 16 moderate expression: see section 08 09 10
thyroid cartilage
weak weak
regionalweak expression: see section 08 09 10 11 12 13 14
trachea cartilaginous ring
weak weak
regionalweak expression: see section 11
axial skeleton cervical region
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14 15
axial skeleton lumbar region
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 13 14 15
axial skeleton sacral region
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13 14
axial skeleton thoracic region
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14 15
cranium
moderate moderate
regionalmoderate expression: see section 01 21
basioccipital bone
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 16 17 18
orbito-sphenoid
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 16 17 18
scapula
moderate moderate
regionalmoderate expression: see section 03 04
sternum
moderate moderate
regionalmoderate expression: see section 11
pelvic girdle skeleton
moderate moderate
regionalmoderate expression: see section 08 09
axial skeleton tail region
moderate moderate
regionalmoderate expression: see section 12 13 14 15
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T7839
Entity Detected:Fam101a, family with sequence similarity 101, member A ( MGI:1920371)
Sequence:sense strand is shown

>T7839
TGGAGCCACAGGCCGGAGCCTCCTTGTTGCTCCTCTGGCTCAGTGGTGGCGGGGACCCGAGCTGCTGGGG
CCCACGCCCCGCGCCCGCCAGTCATGGTGGGTCACCTGCATCTGCAGGCCATGGGGGACACGCGGGAGCA
GAGCCGCGACGGCTTGCTGGACAGTCCCGATTCAGGACTGCCCCCCAGTCCCAGTCCTAGCCCGCCCTTC
TACGCCCTGTCGCCAGGCACCCTGGACACCCGGACCACCACTGAAGCTCCAGCGGCTCCTTCCCTCTTCC
AAACTCCCCCAGCTCTGGAGATGAGGTCACGGTTGCTGCCTGTGTTCTTTGGGGAGAGCATCGAGGTGGA
CCCAGAACCCGCACATGAAATCCGCTGCAACTCTGAGATCACCTACGCCTCAGAGAGATACTTCCGAGAC
AAGATCTTCTACGCTCCAGTGCCCACGGTCACGGCCTACAGTGAGACCATAGTGGCGGCGCCCAACTGCA
CCTGGCGGAGCTACCGCAGCCAGCTGACCCTGGAGCCTCGCCCACGGGCCCTGCGGTTCGGGAGCACCGC
CATCATCTTCCCCAAACTGGCCCGCAGCTCCTTCCGCACCACCCTGCACTGCAGCCTCGGCCAGCCTCGG
CACTGGTACTCCTCCAGCCTGCAGCTGCGGCGGTGCGGGGACCCTACGCCCGGTCCCAGCTGTCCTGATG
TACTCTGAGGAGTCCAGTCTCTGAGGAGGACCTGAGTGTGGGAGAGGTTCAGGAAAGATGGCCCGGCTAC
CACCACAGGTTAGGCTGGCTGTGCCGGAGCTCCAGCCTAGAGGAGTCTGTAAATGCATCTTTCATTAGCC
TTTAGAGGCTCTATGGCCTTAAGGGCAGGGCCAAACATGTCCTGGTGTGCCCTTGAGGCAAAGATGGCCC
TATGTAGCACAGGGCACTCAGCCATGGCCTGGCTCGAAACCTCATTCTTGGAGAGGCTTGTTTCCAAAAT
AGGCCATTAAAAAAAAAAAAAAAACGCTCATTCCAGCTGAGAACCGCAGGTGCGTGCGGAGGGGTTGAGC
TAACTTTACCTTGACCTGAGATGGCAAAGGCTGGGGGTACCCAGAACACATTGTCTGCTGTGCAGGGCGC
AGCCCTCCAGGGCGGCCCACATGTGCCCCACAAGACGCATTCCAGACTAGCTTAGAACTCTTGAGCCTCA
AAGCTCTCTGGCAGGTGCCCTGGGAGGCGTCAAAGCAAATGAGGGGACACGGCCAATAACTGCCTATGGC
TGACTCTGGACTTGAGCCTATGGCAGGGCTCAGAACCCCAAGAGAAGCGGAGTCTGTTTTTCTTGGCCTC
CCGTTCTTGCTTAGGATGGATCCTACAGTTACACCAGTTTCCCACAACCTGGTGCTGTCCCATGGTGCCC
AGGCTAAAATGAGTCCCCGAGTCTACTTTAGAGGTGGAGATGTCCCTTTGTCTCAATTGTCCAGTAGCTA
GAAACCTACCTACTGCCGCTGTGGTGGCCCAGAGGGAAGGGACATTCTTTGTGGGGCAAACAGAACCTAA
ATGACTGATAGATTTCTGTTCATCTATTAGCTTATCTGAGGTGCTCCCTGGTTAAAAGAACAGCCTAAGA
AATTAGTGAATTATCTTAATAAAATATACCCACACAGCCATAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:6336076 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6336076 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14322 same embryo
 EMAGE:14319 same embryo
 EMAGE:14320 same embryo
 EurExpress:euxassay_004115 same experiment
 MGI:4824682 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS