EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:14338

Bnip3 BCL2/adenovirus E1B interacting protein 3 ( MGI:109326)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004164. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004164_01	euxassay_004164_02	euxassay_004164_03	euxassay_004164_04

euxassay_004164_05	euxassay_004164_06	euxassay_004164_07	euxassay_004164_08	euxassay_004164_09

euxassay_004164_10	euxassay_004164_11	euxassay_004164_12	euxassay_004164_13	euxassay_004164_14

euxassay_004164_15	euxassay_004164_16	euxassay_004164_17	euxassay_004164_18	euxassay_004164_19

euxassay_004164_20	euxassay_004164_21	euxassay_004164_22	euxassay_004164_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14338_wholemount_strong.wlz

14338_wholemount_moderate.wlz

14338_wholemount_weak.wlz

14338_wholemount_possible.wlz

14338_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14338_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

forearm mesenchyme

moderate

regional

moderate expression: see section 01 02

upper arm mesenchyme

moderate

regional

moderate expression: see section 01 02 03

forelimb digit 1 mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04

forelimb digit 2 mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04

forelimb digit 3 mesenchyme

moderate

regional

moderate expression: see section 02 03 04 05

hindlimb digit 1 mesenchyme

moderate

regional

moderate expression: see section 05

hindlimb digit 2 mesenchyme

moderate

regional

moderate expression: see section 05 19 20

hindlimb digit 3 mesenchyme

moderate

regional

moderate expression: see section 05 06 17 18 19 20

hindlimb digit 4 mesenchyme

moderate

regional

moderate expression: see section 05 06 17 18 19 20

hindlimb digit 5 mesenchyme

moderate

regional

moderate expression: see section 06 17 18

foot mesenchyme

moderate

regional

moderate expression: see section 02 03 04

lower leg mesenchyme

moderate

regional

moderate expression: see section 01 02 03

upper leg mesenchyme

moderate

regional

moderate expression: see section 01 02 03

mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

anterior naris epithelium

moderate

regional

moderate expression: see section 11 16 17

external naris epithelium

moderate

regional

moderate expression: see section 16 17

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 10 11 13 14 15 16 17 18

heart ventricle

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17

stomach

moderate

regional

moderate expression: see section 04 05 06 07

midgut

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17

clavicle

strong

regional

strong expression: see section 09 10 16 17

axial skeleton tail region

strong

regional

strong expression: see section 13 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7853
Entity Detected:	Bnip3, BCL2/adenovirus E1B interacting protein 3 ( MGI:109326)
Sequence:	sense strand is shown >T7853 CGCCTGGCCTCAGAACTGAGCGGCGCAGGCTGCCCCTGCTACCTCTCGGTGACCGCCTCCCAGGCCTGTC GCAGTTGGGTTCGGGCTCCTTTGCCGAGCCACCATGTCGCAGAGCGGGGAGGAGAACCTGCAGGGCTCCT GGGTAGAACTGCACTTCAGCAATGGCAATGGGAGCAGCGTTCCAGCCTCCGTCTCTATTTATAATGGTGA CATGGAAAAAATACTGTTGGATGCCCAGCATGAATCTGGACGAAGTAGCTCCAAGAGTTCTCACTGTGAC AGCCCACCTCGCTCCCAGACACCACAAGATACCAACAGAGCTGAAATAGACAGCCACAGCTTTGGCGAGA AAAACAGCACTCTGTCTGAGGAAGATTATATTGAGAGAAGAAGAGAAGTTGAAAGTATCCTGAAGAAAAA CTCAGATTGGATATGGGATTGGTCAAGTCGACCAGAAAATATTCCCCCCAAGGAGTTCCTTTTTAAACAC CCGAAGCGCACAGCTACTCTCAGCATGAGAAACACAAGCGTTATGAAGAAAGGGGGAATTTTCTCAGCAG ACTTTCTGAAGGTTTTCCTTCCATCTCTGTTACTGTCTCATCTGCTGGCCATTGGCTTGGGGATCTACAT TGGAAGGCGTCTGACAACTTCCACTAGCACCTTCTGATGAAGATTTGGATCTGACTTCTGTTCACACAGT GAGGATTCATGTCTGAGCTGTGACAGCTAATTGAAGAGCTAGCATGGTCCTTGGGTGTTTGCACTATGTG TGTTAATTTGTTCTGTAAATGCGGTGTTCCTGATTTAGTGAGACAGAATAGACTCTTGTCATGACCTATA ATTATACCTATGGGATCAATTAATAAGCATGTCAGACTGATGCCTATTGTAGCATTTACTAGTAAATTTC TTTGAATATTAGATATAAATAGTAAATTTCTTTGAATATTAGATATAAATAGTATAAATAATTTTAATAT AGTAGCAATGGTGTATTTAAAACTGATCTGTAATCATAATCCAGTTAAGACACTTTACACTTCAAAAGAA CAAGTAAGTGATATTAAAATCATTAAAGGGTTTTCCCCAAAGGAATATTGTGGCCTTATAATCCTATTAC ATAGCAGAAAATTAAAGGGTGCGTGCGGGTTATCTGTAAAGGCCTCTAACTTTGTGAACTGAGTAGCAAG TAGAAGCTAAGGTTACCCACGAACCCCACTTTGCAGTTCCCCCTCGTCCTGCTTTGCAGGATGAGGATTC TCGCCTTGCTGTCCACCATTACCTTGGGTGGTCAGTTTTGTTGCAGGCACCTTTATCACTCTGCTGAATT CTCTTGTTTGTATTTAAATCTTATGTGCTCTGAAAATCAGCTGTCTACTTTAAAAGACTGGAAATGAAAA ACAAAATCTTTGCCAAATCCACTGGAGAATACTATATTTGCATATCCATTAATCAGGATCCAAAGCTGCT TAAATCGGGCGTCCTCCCAGTCTTCTCTTTTCTATAGCATGGTTTTAAAGCCTGCCTCCTTGACATGCTG TATATATCCTGTTGTATTTGTTTCACTGTCCCACACTTAACTCAGGTGTGCTAAAATAAAAGTGACATTT TAACAGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6390074 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6390074 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14337 same embryo
	EMAGE:14333 same embryo
	EMAGE:14336 same embryo
	EMAGE:14335 same embryo
	EMAGE:14334 same embryo
	EurExpress:euxassay_004164 same experiment
	MGI:4823489 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source