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EMAGE:14343

Scara3 scavenger receptor class A, member 3 ( MGI:2444418)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14343 EMAGE:14343 EMAGE:14343 EMAGE:14343 EMAGE:14343
"Pseudo-wholemount" of euxassay_004188. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_004188_01 euxassay_004188_02 euxassay_004188_03 euxassay_004188_04
EMAGE:14343 EMAGE:14343 EMAGE:14343 EMAGE:14343 EMAGE:14343
euxassay_004188_05 euxassay_004188_06 euxassay_004188_07 euxassay_004188_08 euxassay_004188_09
EMAGE:14343 EMAGE:14343 EMAGE:14343 EMAGE:14343 EMAGE:14343
euxassay_004188_10 euxassay_004188_11 euxassay_004188_12 euxassay_004188_13 euxassay_004188_14
EMAGE:14343 EMAGE:14343 EMAGE:14343 EMAGE:14343 EMAGE:14343
euxassay_004188_15 euxassay_004188_16 euxassay_004188_17 euxassay_004188_18 euxassay_004188_19
EMAGE:14343 EMAGE:14343 EMAGE:14343 EMAGE:14343 EMAGE:14343
euxassay_004188_20 euxassay_004188_21 euxassay_004188_22 euxassay_004188_23 euxassay_004188_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14343Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14343_wholemount_strong.wlz
14343_wholemount_moderate.wlz
14343_wholemount_weak.wlz
14343_wholemount_possible.wlz
14343_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14343_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
head mesenchyme
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 17 18 19 20 24
body-wall mesenchyme
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 14 15 16 17 18 19 20 24
cochlea
strong strong
regionalstrong expression: see section 06 07 08 09 16 17 18 19
mandible
strong strong
regionalstrong expression: see section 04 05 06 07 08 09 10 11 12 13 16 17 18 19 20
upper jaw skeleton
strong strong
regionalstrong expression: see section 13
maxilla
strong strong
regionalstrong expression: see section 10 12 13
clavicle
moderate moderate
regionalmoderate expression: see section 06 07 08 09 15 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T7887
Entity Detected:Scara3, scavenger receptor class A, member 3 ( MGI:2444418)
Sequence:sense strand is shown

>T7887
CGCGCAGAAGGGACCATGAAAGTGAGATCAGCTGGCAGTGACAGGGATGTACTGTGTGTCACTGAAGAGG
ACCTGGCTGGTGAAGACGAGGACATGCCATCCTTCCCATGCACCCAAGAGGGCCGGGCAGGACCCCGCTG
CAACCGCTGCCAGAAGAACCTATCTTTGCACACATCAGTGCGGATTTTGTACCTCTTCCTCACTCTGCTC
CTGGTGGCCGTGGCTGTGCTGGCCTCTCTGGTTTTTAGAAAGGTGGACTCTCTCTCAGAAGATATCTCCT
TGGCCCAGTCCATCTATAATAAGAAGCTTGTGTCAATGCAAGAAAATCTTCAAGGACTAGACCCAAAAGC
CCTGATCAATTGTTCCTTTTGCCGTGAGGCTGAGCAGCTGGGGCAAGAGATCAGGAAGGTCCAGGAGGAG
CTGGAGGGGCTTCAGAAGATGCTGCTAGCCCAGGAGGTCCAGCTGGACCAGACCTCCCAGACCCATGAGC
TGCTCTCCACCAGAAGCAGTCAAATCTCCCAGGAGATGGGCAGTTGCTCCTTCTCCATTCACCAGGTCAA
TCAGTCCCTGGGACTCTTCCTGGCACAGGTGAGGGGCTGGCAGGCCACCACAGCAGGCATGGACATCACC
CTGAAGGACCTCACCCAGGAGTGCTATGATGTCAAGGCTGCTGTACACCAGATCAACTTCACGGTGGGCC
AGACTGCTGAGTGGATCCACGGGATCCAAAGGAAGACAGATGAGGAGACCCTGACCCTGCAGAAGATAGT
CACTGACTGGCAGAACTACACCAGACTCTTTGGGGGTCTGCGAACCACCTCTGCCAAGACTGGCGAAATA
GTCAAGACCATTCAGACCACCCTGGGGGCCTCCTCACAGCGCATTAGCCAGAATTCTGAGAGCATGCATG
ACTTGGTTCTGCAGGTCATGGGCCTACAGCTACAGCTGGACAACATCTCTTCCTTTTTGGATGACCATGA
GGAGAACATGCATGACCTCCAATACCACACCCGATATGCCCAGAATCGCACAGTGGAAAGGTTCGAGTCT
CTGGAAGGACGTATGGCTTCTCATGAGATTGAAATCGGCACTATCTTCACCAATATCAATGCCACCGACA
ACCACGTGCACAGCATGCTCAAGTACCTGGACGACGTGCGGCTGTCTTGCACACTGGGCTTCCACACCCA
TGCTGAAGAGCTCTACTACCTGAATAAATCCGTCTCCCTCATGCTGGGCACCACAGATCTGCTCAGGGAG
CGCTTCAGTCTGCTCAGTGCCCGGTTAGACTTCAATGTCCGAAACCTCTCCATGATCATGGAGGAAATGA
AGGCTGTGGACACACACCACGGAGAAATCCTTCGCAATGTCACCGTCATCCGAGGTGTCCCCGGCCCTCC
AGGACCAAGAGGACTCAAAGGAGATACAGGCGTGAAAGGACCTGTTGGTAGCAGAGGACCTAAAGGGGAT
CCTGGCAATCTGGGACCCCCAGGACCTCAGGGTCCTCAGGGGCAGCCTGGGGAGCCAGGACCTGTGGGAG
AAAGAGGGCCAGCTGGCCCCAGAGGCTTTCCAGGCCTCAAAGGCTCAAAGGGCAGCTTTGGCACTGGAGG
ACCAAGGGGGCAGCCAGGCCCCAAGGGGGATGTGGGGCCCCTAGGGCCAGAGGGGCCCCCAGGGTCTCCA
GGACCTTCAGGGCCTCAGGGAAAGCCAGGAATTTCAGGAAAGACAGGGTCACCAGGCCAGCGAGGGGCCA
CAGGGCCTAAGGGTGAACCAGGCATCCAGGGTCCCCCTGGACTACCTGGACCTCCAGGTCCACCAGGGAA
CCAGAGCCCCTACTGAAAAAAGTCTTTGGCCTGATGCTGCCATGCAGGTTGGAGAGGTGAGGGTCAGAGA
AAGCAGCCAGAAGGGTTGCTACAATCATTGTTCTTTGATCCTAATACGAGGGTGTCTCTTAGAGCTGACT
GCATAGCCTTAAGCTTCCCAATAATGACTGTACCATAGGAAAACAGCTCACCTCCCCCTACTTTTTTCTG
CTGACTAAAGGGGAGTCTATTGGGTTTCTATGGCTGGTTAGAAGGAAAGGCTGTGAGAGCCTGCTGGTGG
GGGAAGGGATAGCTACCTGGGGAAGAATGTCTTAGATCTCTTGGATGGTAACTACTCTGGTCTCTGCAAT
GACTTAACACCCATGCGAGCCAAGTCTTGAAGAAAAAAAAAATCCAGGGAAACTTTTGGGGCATTATAAA
TGAAACTCCAGGGATAGCAGCCTCCCCTCTTTCCCCCTCCCTCGACACATAACAGTCACGGTAGAATCAT
GTGGAGCTGCCTTGATCCACACAGGGCAAGAGAAGTCTGCCTGGCTGGCATTCCCAGGCCTTATGCATGG
ATACTGACCCTGACAAGTTCAGAGAGTTTTTATTCACATGTCCAGAAAATTCCTCTTTTCAGTCTTACCT
CTGCATCTCAAGAGTGGCCTGGAAGAAACCCAAGCAGAGTTTCATCGGCAGGTCAAGAACCTACAGGTCC
CAGAGCACTCTGCTTGACTTCTATCCAGCTCCCAGGAAGACCACAGGGCTGGAGGTGGGAGGACATATCA
GGGGAAAACTCCCCTCTCCAAAGCCACCACTTGTTCAACCTACTCTGCCTTGTCTTACTCCCAGTAATAG
CCAAGGAGGAAGAAGCTGGTAACACGGCAGCTCTTGCCTCCTCCTCTTGGTTGACCTGAGACAGATGCAC
AATGACCTCACCCCACAATGACCTAAGATGGCTGGAGTTCTTTCCCACATGAACAGCAAGCTGGACCAGG
CATGCGAAAGGCTGAACGTGCACAAGGAACCTTAGATGTTACTTCTCGTCTTTCATGAACTTCTTGAATA
CACTGGCAGAAAGGGATGCTGGGAAAGGCCTGCATTTCCTTGACTCATTTTGTAGAGCTGCCCTGATCCT
TCATACTCCAGCAGACTCAAACACTCCTGGGCAGAGGCTAGAGCTGTGTCCTCTCTTGCCCTGCCTCAGA
GACCTGTCTTCTTCTTCCTGTTCAAAGGCTCTGTGCTACCTTCTTCATCAATATCAGTGTGCAACTGGCT
TGCACGACCCATCTACCCCTGCTGTATAATTGTCCTGTCTACATATGAAAATATCATGCAACACAATATC
GCTGACCCAGTAAAGAGCTGTTTCCAGTAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:6508271 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6508271 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14339 same embryo
 EMAGE:14340 same embryo
 EMAGE:14341 same embryo
 EMAGE:14342 same embryo
 EMAGE:14344 same embryo
 EurExpress:euxassay_004188 same experiment
 MGI:4827886 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS