EMAGE mouse gene expression spatial database

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EMAGE:14357

Gxylt2 glucoside xylosyltransferase 2 ( MGI:2682940)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004176. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004176_01	euxassay_004176_02	euxassay_004176_03	euxassay_004176_04

euxassay_004176_05	euxassay_004176_06	euxassay_004176_07	euxassay_004176_08	euxassay_004176_09

euxassay_004176_10	euxassay_004176_11	euxassay_004176_12	euxassay_004176_13	euxassay_004176_14

euxassay_004176_15	euxassay_004176_16	euxassay_004176_17	euxassay_004176_18	euxassay_004176_19

euxassay_004176_20	euxassay_004176_21	euxassay_004176_22	euxassay_004176_23	euxassay_004176_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14357_wholemount_strong.wlz

14357_wholemount_moderate.wlz

14357_wholemount_weak.wlz

14357_wholemount_possible.wlz

14357_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14357_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 01 02 03 04 05 15 16 17 18 19 20

radius

strong

regional

strong expression: see section 01 02

ulna

strong

regional

strong expression: see section 01 02

humerus

strong

regional

strong expression: see section 01 02

fibula

strong

regional

strong expression: see section 02

tibia

strong

regional

strong expression: see section 01 02

femur

strong

regional

strong expression: see section 02 03 04 19 20

mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

aorta

strong

regional

strong expression: see section 07 08 09 10 11 12 13

tongue muscle

strong

regional

strong expression: see section 13 14 16 17

mandible

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 16 17 18 19 20 21

lower jaw incisor

strong

regional

strong expression: see section 13

maxilla

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 15 17 18 19 20 21

upper jaw incisor

strong

regional

strong expression: see section 13 14

axial skeleton

strong

regional

strong expression: see section 05 06 07 14

basioccipital bone

strong

regional

strong expression: see section 11 12 13

basisphenoid bone

strong

regional

strong expression: see section 11 12 13

temporal bone petrous part

strong

regional

strong expression: see section 02 03 04 19 20

orbito-sphenoid

strong

regional

strong expression: see section 02 03 04 05 06

clavicle

strong

regional

strong expression: see section 04 05 06 07 08 15 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7892
Entity Detected:	Gxylt2, glucoside xylosyltransferase 2 ( MGI:2682940)
Sequence:	sense strand is shown >T7892 GCCACCGCTAGCTTCGACCCTAGTCCGGGGCGAGCCGGCCTGGACGCTGCGCCGCCGCGGGCAGCGATGC GCAGAGTGTCCGCGCCGCCCCGGGGCCGCCGTGCGGCACCATGAAGCTCCGCAGCAAGGCGGCAGCGCTG CTCCTGCTCGCGCTGGCCGTGCTGCTGCTAGCGCTGCTGTCCTTGCGCGCCCGCCGGGACCCGGAGCCCC CTGGGTTCCCCGCGCGCCCGGAGGCTGCCCCTCAGCGCCGCCACGCGCCGGTCCCCACGCTCCCTCCGGA GCCCCGCGCCTTCCCCGGAGCCGCTGGCCGGCGCTCCCCCAGGCGCCAGCCCCCACGTCTGCGCCCCCGA GCGGGCCGCCCGCGCGCCGCCAGCCGCGAGAAGTTGGCGAGGAGGCCTGGGGAAACGAGGAGCTTGCACT CTGTGCCACCCGAACTCTGGATTCACCTGGCCGTGGTGGCCTGTGGCAACCGGCTGGAGGAAACTCTGGT CATGCTCAAGTCAGCTGTGCTTTTCAGCCACAGGAAGATGCGCTTTCACATCTTCACGGAAGATGCTCTG AAGCCCGAATTTGATAAACAGTTGCGCCAGTGGCCCGACTCCTATACAAAGAAGTTTGAGCACAGACTCT ACCCTATCACATTTTCCGTCGGAAACCCTCAGGAATGGAAGAAACTATTCAAACCCTGTGCCGCCCAGAG GCTCTTTCTCCCGGCGATCTTAAAGGACGTGGACTCGCTTCTCTACGTGGACACTGACGTCCTCTTTCTG CGGCCTGTGGACGACATCTGGAAGCTTCTGAGGCAGTTTAACTCCACCCAGCTTGCTGCTATGGCCCCAG AGCATGAAATCCCGAAGATCGGCTGGTACAGTCGCTTTGCCCGGCATCCTTTCTACGGCTCTGCCGGGGT TAACTCCGGGGTCATGCTCATGAACTTGACTCGCATAAGAAATACCCAGTTCAAGAACAGCCTGATTCCA GCAGGCCTGGCTTGGGAGGAGATGCTGCTTCCACTCTACCAGAAGTATAAAAGTGCCATCACATGGGGAG ACCAGGATTTATTAAATATTATTTTTTATTACAACCCAGAGTGTCTCTATGTGTTCCCCTGCCAGTGGAA CTACCGCCCTGACCATTGCATGTATGGAAGCAACTGCAAAGAGGCTGAACGGGAAGGCGTGTCTGTTCTG CATGGCAACCGGGGTGTCTACCACGACGACAAACAACCCACTTTCCGAGCGCTTTATGAAGCCATCCGGG ATTTTCCCTTTCAGGACAATCTGTTTCAATCTATGTACTACCCTCTGCAGCTGAAGTTTTTGGAGACGGT GCACACCCTGTGTGGACGGATCCCCCAGGTTTTTCTCAAGCAAATTGAGAAGACGATGCGAAGGGCTTAC GAGAAGCACGTCATCATCCACATGGGCCCCAACCCCATGAGCTGAGCCAGTGTCTGGGACCGAGGACGTG GATCCTTACGCTGCCTGGATATTTGTGTGTGTGTGTGTGAATATTTAATGGTGCTCTGTGACTGTTGCGG TTCAAAAGCCTGATTGTATTTTCCTAAGTTAATTATCCCTAAAAGGGAATATGCTTTTCTTTATTTTCTC CTGATATCTCCTGAATCTTTTTATTTTTAAAGTTATTTTATGAATCTTATATTGTCCCAATGGGCTGAGA GAGAAAAGGGGAACTTTTTCTAGCCCTCAAATATGAGCCCCTGTTGATATATGCAGCTATCGTGATAATC TGGATTTCTTGAGAATCTTCCTATGCAACTTGCTCTCTCATACCCTATTAAAAAGCAATACATGTTCAGG CTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6509198 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6509198 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14362 same embryo
	EMAGE:14359 same embryo
	EMAGE:14358 same embryo
	EMAGE:14360 same embryo
	EMAGE:14361 same embryo
	EurExpress:euxassay_004176 same experiment
	MGI:4825289 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source