EMAGE mouse gene expression spatial database

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EMAGE:14408

Mis18bp1 MIS18 binding protein 1 ( MGI:2145099)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004198. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004198_01	euxassay_004198_02	euxassay_004198_03	euxassay_004198_04

euxassay_004198_05	euxassay_004198_06	euxassay_004198_07	euxassay_004198_08	euxassay_004198_09

euxassay_004198_10	euxassay_004198_11	euxassay_004198_12	euxassay_004198_13	euxassay_004198_14

euxassay_004198_15	euxassay_004198_16	euxassay_004198_17	euxassay_004198_18	euxassay_004198_19

euxassay_004198_20	euxassay_004198_21	euxassay_004198_22	euxassay_004198_23	euxassay_004198_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14408_wholemount_strong.wlz

14408_wholemount_moderate.wlz

14408_wholemount_weak.wlz

14408_wholemount_possible.wlz

14408_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14408_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 13 14 18 19 20

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

midbrain ventricular layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7866
Entity Detected:	Mis18bp1, MIS18 binding protein 1 ( MGI:2145099)
Sequence:	sense strand is shown >T7866 GGGGAAGTGGACGAGGAAGGCGAGCGGAGTTTCAGTCTGCGGACACGCGTGGAGCCCTTGCCCGGGCCTC CGTGGGTCTGAGGCGCTGCGAGCCCTGGGTAACCACGGCCTCGAGCTGCTGTCCTCACCAAGGTTTCTCT GGACTCCTTCTGAAGTTCTGTCTGAAATTTTGTGTTGAATGAATTTTCTATAAAAAGAATTTTGTTGCCA CTCTCCTATCAAGATGATTGTAACACCTTTGAAGCATTCAGGAATTCATTTGTCTTCTGGAACATTGCAA AGAAGAAATATGCCTCTAGATGCAGTCTTTATTGACAGCATCCCTTCAGGCACACTCACTCCTTTGAAAG ATTTGGTAAAATATCAGAAGTCCTCTCTAAAAGTTAATGGTCATAAAAAGAATCAGTTATTAGAAATAAG AACTTCTAACAATAAAGATCTATTTCAATCCACCATGTTATCAGAGGCTACCCTTCCAAACAGCTCTCTT GATATCAGTGTTATAAAGCCCAGCATGGATAGATTAAGAAATGAAATGATTTATGAATCACCAGGTAAAA TCTTTCAAAGAATGAAAGCAAAAGTACAGCGTGACAAACAAGAACAACTGACAAGGAGCAGTAGCATGTT GGGATCACCACAGGGTGAACACACCAAAGACTTTCCTCCTAACACGGATAAGAAAGCTCAGTTGCAACAA ACCTATATCTGTGAGGAAAAACAAACGTCGGTCCAATCCAATGACCCTTCATTGGGAGATCCTCCAATTC TGAACCAAGAACAAAAAAATGTTTCAGCTTCGTGCATTTCAAAGAAGGCATTAACTAGAGCCCAGTTTGG CGGACAAGTTCTTCATTCAAAAGAGAGTCCTGTTAGGATCACTGTGTCCAAAAAGAACACATTTGTTTTG GGAGGCATTGATTGTACTTATGAAAAGTTTGAAAATACTGATGTTAACACCATTAGTTCTCTTTGTGTTC CTATTAAGAATCATAGCCAATCTATTACTTCTGATAATGATGTGACAACAGAAAGGACTGCAAAAGAGGA TATTACAGAACCAAATGAAGAGATGATGTCCAGAAGAACTATTCTTCAAGATCCCATAAAGAATACATCT AAAATTAAACGTTCAAGTCCAAGACCTAATTTAACACTATCTGGCCGGTCTCAAAGAAAATGTACAAAGC TTGAAACTGTTGTAAAAGAAGTAAAAAAATATCAGGCAGTCCACCTACAGGAATGGATGATTAAAGTCAT CAATAATAATACTGCTATATGTGTAGAAGGAAAGCTGGTAGATATGACTGATGTTTATTGGCATAGCAAT GTAATTATAGAGCGGATTAAACACAATGAACTTAGGACCTTATCAGGCAACATTTATATCTTAAAAGGAT TGATAGACTCGGTCTCCATGAAAGAAGCAGGATATCCCTGTTATCTCACAAGAAAATTTATGTTTGGATT TCCCCACAACTGGAAGGAACACATTGATAAATTTCTAGAACAATTAAGGGCTGAAAAAAAGAACAAGACC AGACAGGAAACAGCAAGAGTCCAAGAAAAACAAAAATCAAAAAAAAAAGATGCAGAAGATAAAGAAACTT ATGTCCTCCAAAAGGCCAGCATCACGTATGACCTTAATGATAATAGCTTAGAGAGAACTGAAGTACCCAC TGATCCCTTGAACTCACTGGAACAGCCTACCTCCGGCAAAGAAAGAAGACACCCGCTTCTCAGTCAGAAG AGAGCTTATGTTTTAATAACACCACTTAGAAACAAAAAGTTGATAGAGCAAAGATGTATAGACTACAGTC TCTCTATTGAAGGAATATCGGACTTTTTCAAAGCAAAGCATCAAGAAGAAAGTGACTCAGATATACATGG AACTCCAAGTTCTACCAGTAAGTCTCAAGAGACCTTTGAACATAGAGTGGGATTTGAAGGCAATACCAAG GAGGACTGCAATGAATGTGACATAATCACTGCCAGACATATTCAGATACCTTGCCCGAAAAGTAAACAAA TGCTCACCAATGATTTTATGAAAAAGAACAAGTTGCCCTCAAAACTGCAGAAAACTGAAAATCAAATAGG TGTATCACAGTATTGCCGGTCCTCATCACATTTGTCAAGTGAAGAGAATGAAGTAGAAATTAAAAGTAGA ACCAGAGCAAGAAATACAAAAGAAAGGCTGAATCGGGAGAGAGAAAACACAAACCACATCACAAAAGATA TCCTCCTCATTTCAGAAACTGAAGGTGAGAGAGCTTGTTATATCACACCAAAGAGACCTCGATCTTGTTA TATCACACCAAAGAGACCTCGATCTTCTGCTAAAGAGTCACATTACAAATCTGCTGTTAGCAAGGACTTT CTCACTGAAGGAAAGGCATCTGATAGAACTAGCAGACAGCTGTTAGACCATCTACCTGGTTTAACTGATG ATGAAGAATGGAGTGAGCAAGAGTTACAGAAGCTACATTGTGCTTTTACATCTCTTCCAAAGCACAAACC TGGCTTTTGGTCAGATGTGGCTATGGCAGTAGGTTCTCGAACTGCTGATGAGTGCCAGAAGAAATACACA GAAGAACCCCAAGGCCAAGGATCCCGAAAACATGGCTCTAAGAAAAAGCAAGCCAACAAGGTCCAAAATG GTGAGAAAGACAGTGCTGATGCCAAGACTATTAAGATAACTGCCAAAGTGGGAACTCTTAAAAGGAAACG ACAAATGAGGGATTGCCTAGAACATTTGGCAAAAGACAACCATGATGATTTTTTCACTGCAACACCCTTG CAGAAACAAAGAATTCAGTTGCCAAGTTTTCAATACAGTCAAGATGATGATTTTCTGCTAGATATGGACA GGGATCCAGCATCTCCATCATCAATTATTACTTCACCATTGAGAAGCACTACTCCACAATGTCAGCATTT CAGCCCCAGCATGCTAGCCGCTATAGAAAGGAATAATTGTGATAGATATGTTTACCAAATGCAAAAAAAT GCTAAAAAATATGGCAAGAGTAATGGTGGCCTTGTCTGGGGCAACATTAGGAAAAAAACAGTTAAAACTG ATCTCTCATCTCCACCACCAACACGGAAAGCCCTGTTTAACAAAGATTTAGGTAAAAACACTGATATTTC AAAGTATTTCATTGATGATACAGAATCAGATGAAGAAGAGAAAGATTATTACTTTTCCAATTCTGACTGA TAGACGACTTGCAGGAATTTTACCATCAAGCAGAATATACTTTTATCCTCATTGATGACTATTTTCTTTG TAATAAGGGAATGCAGATTTGTTTCCCAATGTCTTATGTTTGTATGCACCCTATAGAGCATTCCTCAGAA CTACAGCTTACAAATACTTTGCCTATGTGTGCATTTGTAAATTTTATGTTTGTAATATTAAAGTATATAA AGTTCAGTCTCTTGAGAACATCCAAGCCTGTTTTCTTTAGTAATCATGTCTCAAGTAGAATTTTAATTGA ACTTTTAAAAGGAGGCCTTAAATGAGGAGAGGTAAGGGGCCAGAGTTAAGATTGTGGACTGGAAGATACT AGAATGAAAACTTTTAGCATCATGCAGCCTGAAATTACAAGCCAGGGTTCCTTCTGTTTAAATTATGTCA TTCAAACTGTTTTACCTGGTTTTTATGTTTTACCTGCTTTCTCTTCTGATAGTTTTTCTTAAATTTTTTA CTGTATGTTGGTCTGTTTGTGAAAACTTCACCTCTAATTCATTGTTCTTTATTATTTTATAAAGAAAGCT GTGGACAGGTTCTGTTGTATTCTTCAGCCCTGCTATATCTATCTGATAGATCCACCCTTACAAGGACTTA CAATGCCATAATGAGCAGGGTTTCCCTTTCTTACCGCTTCAAAAGGTAACTTGCCTTCTGCCGTTGTTTT AACATTTCCCCTAAGTTATTTGTCCTCTGCTATAGTAGTATTCATCCACCCTTTAAATCTAGTTAGACTG CGCAAATAAAAATGTACATTTGTGAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6394068 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6394068 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14407 same embryo
	EMAGE:14412 same embryo
	EMAGE:14411 same embryo
	EMAGE:14410 same embryo
	EMAGE:14409 same embryo
	EurExpress:euxassay_004198 same experiment
	MGI:4823562 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source