EMAGE mouse gene expression spatial database

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EMAGE:14689

Itgb6 integrin beta 6 ( MGI:96615)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005620. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005620_01	euxassay_005620_02	euxassay_005620_03	euxassay_005620_04

euxassay_005620_05	euxassay_005620_06	euxassay_005620_07	euxassay_005620_08	euxassay_005620_09

euxassay_005620_10	euxassay_005620_11	euxassay_005620_12	euxassay_005620_13	euxassay_005620_14

euxassay_005620_15	euxassay_005620_16	euxassay_005620_17	euxassay_005620_18	euxassay_005620_19

euxassay_005620_20	euxassay_005620_21	euxassay_005620_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14689_wholemount_strong.wlz

14689_wholemount_moderate.wlz

14689_wholemount_weak.wlz

14689_wholemount_possible.wlz

14689_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14689_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

moderate

regional

moderate expression: see section 01 02 03 04 05 06 15 16 17 18 19 20 21 22 weak expression: see section 14

arm

moderate

regional

moderate expression: see section 01 02

hand

moderate

regional

moderate expression: see section 03 04 05 06

foot

moderate

regional

moderate expression: see section 01 07 08 21 22

leg

moderate

regional

moderate expression: see section 03

lower leg

moderate

regional

moderate expression: see section 02 04 21

upper leg

moderate

regional

moderate expression: see section 02 04 21 22

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

tongue muscle

moderate

regional

moderate expression: see section 12 13 14 15

stomach

strong

regional

strong expression: see section 05 06 moderate expression: see section 01 02 03 04 07 08 09

rectum

moderate

regional

moderate expression: see section 12

midgut

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17

bladder

moderate

regional

moderate expression: see section 12

lung

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

tail mesenchyme

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5562
Entity Detected:	Itgb6, integrin beta 6 ( MGI:96615)
Sequence:	sense strand is shown >T5562 TGTTTTCATTTTAGTCTTAGTGAAAGGTTTTGAATTTACAGCTCAAGTTACTTTTCAAAGCAGTGTGAGT AGTTTTACTTAAAAAAACAAAACAAAACCAAAAACCCCGGCAAGAAAGACTTTCACGGCTTCCAGCTTTG GTCTTGTAACCCTGAAGGTAATTCATTTTTAATCTGTCTGCACAGTGAAAACTGAACCGAATGGGGATTG AGCTGGTCTGCCTGTTTCTGCTACTTCTAGGAAGGAATGATCACGTCCAAGGTGGCTGTGCCTGGGGTGG TGCAGAAAGCTGCTCAGACTGCCTGCTCACAGGACCCCACTGCGCCTGGTGCTCCCAGGAGAATTTCACC CACCTGTCTGGAGCTGGCGAGAGGTGCGACACCCCAGCGAATCTTTTAGCCAAAGGATGTCAACTACCCT TCATTGAAAACCCTGTCTCCCGCATAGAAGTCCTTCAAAATAAGCCTCTCAGCGTGGGCCGACAGAAGAA CAGTTCTGACATTGTTCAGATTGCTCCTCAAAGCTTGGTTCTTAAATTGAGACCAGGGCGTGAGCAGACT CTGCAAGTGCAGGTCCGCCAAACTGAAGATTACCCAGTAGATCTGTATTACCTCATGGACCTCTCCGCCT CTATGGATGACGACCTCAACACCATCAAAGAGCTTGGCTCCCGGCTGGCCAAAGAGATGTCCAAACTAAC CAGCAACTTTAGACTGGGCTTTGGCTCTTTTGTGGAAAAGCCCGTTTCTCCTTTTATGAAAACAACCCCA GAGGAAATCACCAACCCTTGCAGTAGTATCCCCTATTTCTGCTTACCTACATTTGGATTCAAGCACATTT TGCCATTAACTGATGATGCCGAGAGATTCAATGAAATTGTGAGGAAACAGAAGATTTCTGCTAATATTGA CACACCTGAAGGTGGATTTGATGCAATCATGCAGGCTGCTGTGTGTAAGGAAAAGATTGGCTGGCGCAAT GACTCGCTCCACCTCCTGGTTTTTGTGAGTGATGCCGATTCTCATTTTGGAATGGACAGCAAGCTGGCAG GCATTGTCATTCCCAATGATGGACTCTGTCACTTGGACCACAGGAATGAATATTCCATGTCAACTGTCTT GGAATATCCAACTATCGGCCAACTCATTGATAAACTGGTACAAAACAACGTGCTACTGATCTTTGCAGTC ACCCAAGAACAAGTCCATCTGTATGAGAACTATGCAAAACTCATTCCTGGAGCAACCGTGGGACTGCTTC AGAAGGATTCTGGGAACATCCTTCAGCTGATCATCTCCGCTTATGAAGAACTGCGGTCTGAGGTGGAACT GGAAGTGTTAGGGGACACAGAAGGACTCAACCTGTCTTTCACAGCTCTCTGTAACAATGGTGTCCTCTTC CCACACCAAAAGAAATGCTCCCACATGAAAGTTGGAGACACAGCATCCTTCAATGTGACTGTGAGCGTAT CCAACTGTGAGAAAAGAAGCAGGAACCTCATCATAAAGCCAGTGGGGCTGGGGGACACCCTAGAAATACT CGTCAGTGCAGAATGTGACTGCGACTGCCAGAGAGAAATAGAAACTAACAGCTCTAAGTGTCACAATGGG AATGGCTCCTTCCAGTGTGGGGTGTGTACCTGCAACCCCGGCCACATGGGTCCTCACTGCGAGTGTGGTG AGGATATGGTGAGCACGGATTCCTGCAAGGAGTCCCCGGGTCACCCTTCATGCAGTGGAAGGGGTGACTG CTATTGTGGGCAGTGCATCTGCCACTTATCGCCGTACGGAAGCATCTACGGACCTTACTGCCAGTGTGAC AACTTCTCCTGTCTGAGACACAAAGGCCTTCTCTGTGGAGATAACGGTGACTGTGACTGTGGCGAGTGTG TGTGCCGGGATGGCTGGACAGGTGAATACTGTAACTGTACAACCAACAGGGACTCGTGTACATCTGAGGA TGGAGTGCTGTGCAGTGGACGTGGGGACTGTGTCTGTGGCAAGTGTGTCTGCAGAAACCCTGGAGCCTCA GGACCCACCTGTGAACGCTGTCCTACCTGTGGCGACCCCTGTAACTCTAAACGGAGTTGCATCGAGTGCT ACCTGTCTGCAGATGGCCAGGCCCAAGAAGAGTGTGCTGACAAGTGTAAAGCCATTGGTGCCACCATCAG TGAGGAAGATTTTTCAAAGGATACTTCTGTCTCCTGCTCTCTACAAGGAGAAAATGAATGCCTTATTACA TTCCTAATAACTACGGATAATGAAGGGAAAACCATCATTCACAACATCAATGAAAAAGACTGCCCCAAAC CTCCAAACATTCCCATGATCATGTTGGGGGTGTCACTGGCGATCCTGCTCATCGGAGTTGTGCTACTGTG CATTTGGAAGCTGCTGGTATCATTTCATGACCGGAAGGAAGTTGCTAAATTTGAAGCAGAACGCTCTAAG GCCAAGTGGCAAACGGGAACCAATCCTCTGTACCGAGGTTCCACCAGCACTTTTAAGAACGTGACCTACA AGCACAGGGAGAAGCACAAAGCAGGCCTTTCCTCAGATGGGTAGACAGACCTCCTTCGGCCAGAGAAAGT CAACTCCGCTTGCGTTAAAGGTTTACTGCACTGTATGGTTTATCATGCTTTGTCGCTTCAAAAGGAGGTC GACTTAAGATAATAACAGGTCATTCAACAACCAGTCATTCAGGGCATGAAGGTGAGACATTGTTCGCTGA TTGGAAACAATCAAGAAAAGAATATCCCAAGCAAAGAGATGAATTCAAGATCCCTTGAAGAATATAAACT CTGTTGCATTAATGCCTCAAAAAAAAAAATCATCAAAACGATTCCCGGGAACTTGATTTGCATTTGAAAA ATGTTCAGAATTAGAATCTCATTTGCTTCCGGAATCCAGCTACCTAAAGTCTTTGTCTCCAAAAAAATCC CGGAGTCCTTATGCATTGTCTACCCATACTTGCAGAAAAATCTATTTCCTGAAGGAATTTGTTTCTTTGG TTGGTTTGGTTTGGTTTTGTTTTTTTATTTCCTTTCCTTTTCTTTTTTTCTTTTTTCTTTCTTTCTTTCT TTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTTTCTTTTTTTTTTTTTTTTAGAAAGGGTGTCT CTATGGCCATTCTGGAACTGACCAGGCTGCCTGGCCTTGAACTCACAACCATGGAACTGCCTGTCTCTGC CTTCCATTTCATGCTCAGAGATCTTAACTTTTATGTATGAGAAATGAGAGTCTGATTTCCCATCAACTGA AAGAATCCAGTTCTCCCAGAGTGACTTCCCAAGTGAGAGATTGAAAATTAAGTCATGGGCTTGTACTCTT AAAAGACATGGACACAAGTATTTTGTTTGAAAGAGGAGGAAGGGGTAATTTATAAAATGTGTTGCTAGTA ACACTAAAGATATGGGTCCTCCGTGTTCAATTGCCCTCTGATCCCCAAGTTTCCCTCCTATTATTCCACA TTTCTCAATTATTGCACTCAACTACAGTCTTCAAAACTCCACAACTGTCATACAAAAGTATCCACTTTTT AAAAAATCACTTTAAGTTATTCGCTTGTCAACTAGAATTTTGAGAATAGAAAAGAAACCTAGGGTAACAG AATCAAAGGCTACACAGCTTTATATCAAATGTGTCAAGACTGTGGAAGTCCCGCATGAAGTCTGCCCTAC ATGTAGGACATTCCTATCCCGTATGTACCACCCAGAACACACATCCCAGCTTCAGGAGGTGGGCATGCCA CCCACTTGCTCTAAAAAGTAAAACCACTTACAAAATCTATGCAAGGCAACAAACAAAACCGGTAGAAGAT TGGAGGGATAGTCTCTGGAGGGTTTCATTTCTTATAGTTACTGCTGCATTACAAACCACTTTGATCTCAC TGGATCTCCATAGAGATGCTGAGAGGATGGGCAGAAGAGGTGGGTACAGACTCTCAATCTTATAACCCCA AGCCCTTTGTTCTCTCTACTGCACGAGACTCTTTTGTCAGTAAAATGATGGAAGGATTGTATTAGTTATG GTATAACTGTCTTAGTTTGAAAGTATACTGACACAAAACAATTAGCACCTAGGCCCCCGGAATGTGGACC GGTTGCTGTATTTTTGTGCTGTGTGTGTTGCCTTCACTGGATATTAAGGTGAATCCACTCCCTAGGTGAA CAGCTCTGTGTTTGTGAGTGGAGGCTTTGACAGCTGTCTGTTGAATTGTCAGCTTGGTCTCAAACCTCCT TCACTCCTGCCATATAGGTCTGTGTACGTGTCCACATCATCTAGGTTCTTTCTTTTCTGGTTTTTAAATG ACTCAGGTAAATTAGTCTGTTAGGGAGTACACAGCTAAATTTAAAAGTCTTAATGAATCTCCTTTGGATC CCTGAATGGTCCAAGATCTTGGAGTCTGGAGCAACCAGCAGATTCACTCCCAGTGATGTGGTGAGACTGC TGAGGATACACGGGGCTGTTTGTGCTGTTTTTGTGTTATTGCTGAAATGAGATGTTAGGAAATAGAAGTT TCTCTCATGTGTGTATCAGGAAGAATTTGTTCTTTCTCTAATTACCGTTTTGACCCAAGGCAGGACCTTG AAAGAGCCAAAACATTAACAGTAGTGCTTCTGTTCACTGAAGAATTATGTTACATGGAGATAAAATGGTT GTTTTTTTTTTTTAAAGTGGTTTTATTGTATTTTGCGTTGACATAAATAAACATAATTTAAACAATGAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6475423 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6475423 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14688 same embryo
	EMAGE:14687 same embryo
	EurExpress:euxassay_005620 same experiment
	MGI:4825656 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source