EMAGE mouse gene expression spatial database

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EMAGE:14736

Axin2 axin2 ( MGI:1270862)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005683. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005683_01	euxassay_005683_02	euxassay_005683_03	euxassay_005683_04

euxassay_005683_05	euxassay_005683_06	euxassay_005683_07	euxassay_005683_08	euxassay_005683_09

euxassay_005683_10	euxassay_005683_11	euxassay_005683_12	euxassay_005683_13	euxassay_005683_14

euxassay_005683_15	euxassay_005683_16	euxassay_005683_17	euxassay_005683_18	euxassay_005683_19

euxassay_005683_20	euxassay_005683_21	euxassay_005683_22	euxassay_005683_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14736_wholemount_strong.wlz

14736_wholemount_moderate.wlz

14736_wholemount_weak.wlz

14736_wholemount_possible.wlz

14736_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14736_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hand skin

strong

regional

strong expression: see section 07

adenohypophysis

strong

regional

strong expression: see section 13 moderate expression: see section 09 11 12 14

skin

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

vibrissa

strong

regional

strong expression: see section 22 moderate expression: see section 08 09 weak expression: see section 07

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 12 13 14

diencephalon meninges

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 not examined expression: see section 04 05 06 07

thalamus

moderate

regional

moderate expression: see section 15

cerebral cortex mantle layer

moderate

regional

moderate expression: see section 15 16 17 not examined expression: see section 21

cerebral cortex ventricular layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 17 18 19 20 moderate expression: see section 03 04 05 06 15 16 21

telencephalon meninges

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 01 02 03 04 07 12 14 15 16 17 18 19 moderate expression: see section 05 06 08 09 10 11

hindbrain meninges

strong

regional

strong expression: see section 01 moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

metencephalon rest of alar plate mantle layer

strong

regional

strong expression: see section 13

metencephalon rest of alar plate ventricular layer

strong

regional

strong expression: see section 01 02 03 04 10 11 12 14 15 16 17 18 19 moderate expression: see section 05 06 07 08 09

midbrain meninges

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

midbrain mantle layer

strong

graded

strong expression: see section 08 09 10 11 12 13 moderate expression: see section 14 15 16

spinal cord meninges

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

inner ear

strong

regional

strong expression: see section 01 moderate expression: see section 02 03 04 05 06 07 14 15 16 17 not examined expression: see section 20

pharyngo-tympanic tube

strong

regional

strong expression: see section 01 moderate expression: see section 02 20 21 22 23

heart

moderate

regional

moderate expression: see section 07 08 09 10 11

tongue epithelium

strong

regional

strong expression: see section 11 12 13 14 moderate expression: see section 15

lower jaw molar

moderate

regional

moderate expression: see section 07 08 19

oral epithelium

moderate

regional

moderate expression: see section 05 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

upper jaw molar

moderate

regional

moderate expression: see section 07 08 19

metanephros

moderate

regional

moderate expression: see section 07 08 09 15 16 17

lung

strong

regional

strong expression: see section 14 moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9175
Entity Detected:	Axin2, axin2 ( MGI:1270862)
Sequence:	sense strand is shown >T9175 GCGCGGCGGGATCACTGGCTCCCCGAGCCCGGCCCGGGGGAGTCGGCTGGAGCCGGCTGCGCTTTGATAA GGTCCTGGCAACTCAGTAACAGCCCAAGAACCGGGAAATAAAAATAAGCAGCCGTTCGCGATGGATTTCG GGGCCACCCGGAGGCCGAGGCGTCCGCCTCCCCAAAGGAGAGCTTTGCTGTAAAAGAGAGGAGGCTCACA TGAGCCCCTGCTGACTTAAGAGAGACCAAGCCGATTGCTGAGAGGAACTGGAAGAAGAAAAAGGAGGAGG AGGGAAAAAAAGCAAAACAAAATCCAAACTCAGTGAGACGCTCTCCCTCACCATGAGTAGCGCCGTGTTA GTGACTCTCCTTCCAGATCCCAGCAGCAGCTTCCGCGAGGATGCTCCGCGGCCCCCGGTTCCGGGAGAAG AAGGGGAGACCCCACCGTGTCAGCCTAGTGTGGGCAAGGTCCAGTCCACCAAACCTATGCCCGTTTCCTC TAATGCTAGGCGGAATGAAGATGGACTGGGGGAGCCCGAGGGGCGGGCCTCCCCCGATTCCCCTTTGACC AGGTGGACCAAGTCTTTACACTCCTTGTTGGGTGACCAGGATGGTGCATACCTCTTCCGGACTTTCCTGG AGAGGGAGAAATGTGTGGATACGCTGGACTTCTGGTTTGCTTGTAATGGGTTCAGGCAGATGAACCTGAA GGATACCAAAACTTTGCGAGTGGCCAAAGCAATCTATAAGAGGTACATTGAGAACAACAGCGTTGTCTCC AAGCAGCTGAAGCCCGCCACCAAGACCTACATACGAGATGGCATCAAGAAGCAACAGATCGGCTCGGTCA TGTTTGACCAGGCACAGACCGAGATCCAGGCAGTGATGGAGGAAAATGCCTACCAGGTGTTCTTGACTTC TGACATTTACCTGGAATATGTGAGGAGTGGGGGGGAAAACACAGCTTACATGAGTAACGGGGGACTGGGG AGCCTAAAGGTCTTATGTGGCTACCTCCCCACCTTGAATGAAGAAGAGGAGTGGACGTGTGCCGACCTCA AGTGCAAACTCTCACCCACCGTGGTTGGCTTGTCCAGCAAAACTCTTCGGGCCACCGCGAGTGTGAGATC CACGGAAACAGCTGAAAACGGATTCAGGTCCTTCAAGAGAAGCGACCCAGTCAATCCTTATCACGTAGGT TCCGGCTATGTCTTTGCACCAGCCACCAGCGCCAACGACAGCGAGTTATCCAGCGACGCACTGACCGACG ATTCCATGTCCATGACGGACAGTAGCGTAGATGGAGTCCCTCCTTACCGCATGGGGAGTAAGAAACAGCT CCAGAGAGAGATGCATCGCAGTGTGAAGGCCAATGGCCAAGTGTCTCTACCTCATTTTCCGAGAACCCAC CGCCTGCCCAAGGAGATGACGCCTGTGGAACCTGCTGCCTTCGCCGCCGAGCTCATCTCCAGGCTGGAGA AACTGAAACTGGAGCTGGAAAGCCGCCATAGTCTGGAGGAGCGGCTGCAGCAGATCCGGGAGGATGAAGA AAAGGAGGGGTCTGAGCAGGCCCTGAGCTCACGGGATGGAGCACCGGTCCAGCACCCCCTGGCCCTCCTA CCCTCCGGCAGCTATGAAGAGGACCCACAAACCATTTTGGACGACCACCTCTCCAGGGTCCTCAAGACCC CCGGCTGTCAATCCCCTGGTGTGGGTCGCTATAGCCCACGGTCCCGCTCCCCCGACCACCACCACCAGCA CCACCACCATCAGCAGTGTCATACCCTTCTTCCGACTGGGGGCAAGCTGCCCCCCGTGGCTGCTTGCCCC CTCCTTGGAGGCAAGAGCTTCCTGACCAAACAGACGACGAAGCACGTTCACCACCACTACATCCACCACC ACGCTGTCCCCAAGACCAAGGAGGAGATCGAGGCAGAAGCCACACAGAGAGTCCGCTGCCTCTGTCCTGG GGGAACAGATTATTATTGCTACTCCAAATGCAAAAGCCACCCAAAGGCTCCAGAGCCCCTGCCTGGGGAG CAGTTTTGTGGCAGCAGAGGTGGTACCTTGCCAAAACGGAATGCAAAGGGCACCGAACCGGGTCTTGCAC TGTCGGCCAGGGATGGAGGGATGTCCAGTGCAGCGGGGGCCCCCCAGCTTCCTGGGGAAGAAGGAGACCG GTCACAGGATGTCTGGCAGTGGATGTTAGAGAGTGAGCGGCAGAGCAAGTCCAAGCCCCATAGTGCCCAA AGCATAAGAAAGAGCTACCCATTGGAGTCTGCCTGTGCGGCCCCAGGAGAACGAGTCAGCCGGCACCATC TGTTGGGGGCCAGCGGACACTCCCGCTCGGTGGCCCGGGCTCACCCATTTACCCAGGACCCTGCAATGCC TCCCCTTACCCCACCCAACACTTTGGCACAGCTAGAGGAAGCCTGCCGCAGGCTGGCAGAGGTGTCGAAG CCCCAGAAGCAGCGGTGCTGCGTGGCCAGTCAGCAGAGGGACAGGAACCACTCGGCTGCTGGTCAGGCAG GAGCCTCACCCTTCGCCAACCCAAGCCTGGCTCCAGAAGATCACAAAGAGCCAAAGAAACTGGCAAGTGT CCACGCGCTCCAGGCCAGTGAGCTGGTTGTCACCTACTTTTTCTGTGGAGAAGAAATTCCATACAGGAGG ATGCTGAAGGCTCAAAGCTTGACCCTGGGCCACTTCAAGGAGCAGCTCAGCAAAAAGGGAAATTACAGGT ATTATTTCAAGAAGGCGAGTGACGAATTTGCCTGCGGAGCAGTTTTTGAGGAGATCTGGGACGACGAGAC AGTGCTCCCCATGTACGAAGGCAGGATCCTGGGCAAAGTGGAGAGGATCGACTGAGCCTTGGCCTCCTCG GCGTGCAACCTGGGCAAGCACCTCGGCGTGCACCATGGAGCCGAAGCCCAGAGACCCTGTCTCAGGCCTA CGCAACAGCCACGAAATATTCTGAAGGAAAATGAAACCAATTAAGAAGACAAAGCCTAGGGAGGGACTGG CGCCTGGGCCTTCAGGAGGGCGGGGGTAGTTGATCTTCAGTCTCCAGGAGCCTGGGTACCGAGATGAGAA AAGCCTGAACTATTTATTCAAAACATGACCACTCTGGGCTATAGAAGATGCTGAGTGTGTTTGAGAGACT GACATACATAATAGATGACTTCCTAGGGTTCTGAAATTCATAGACTAAGAGAAAACTGTGTATAGCTTGC CCGAACAGGAGTCCTTACTGATATTTATTGAACAGTCGATTCCCCTACCCGCCCTCCCTACCCCCAGCCC CCAGTTTATGCTGCTTTTAAACCTGGAAGTGGGAGTGAGAAGTTTGGATTGCTGTCCACGCTTAGGAGCC AAGCCGGGAATGCATTAATCATTTCTTCGTTAACAGAGGAATCTGCTCTGCATGGCATTTCTCCAAGTGA AGACAGGCTTCTTTTTTTTTTTTTTAAATGGTGCCCGCCCCACCCATCGACACATGCAGAAATGGGTCAC CCCCACCCCACCCCACCCCCCGCCATGCTGCTCTGCTTGTCACAGACGTCCTGTGGGTTGGTTGTGACAG CATCTTCACCACTTTGGGGACCATCCAGAGTGGGGGAGTGGGGGAGACTTCACCCTGGAGCCAAAGGCTA CACCGTACGTGTAGTCCCAGAGCCCGTCACAGCCCTTGTGGTTCAAGCTTCTTCTGCCTCTTTAGGAAGT GAGGGTTTCTTGTTCTCCTTTAAAAATCAGTCTCTAGACTACGGCCATCAGGAATCTACCCTCGCCCACC TGACCCTGCGAGGACACGGGCACCCAGCAGCACCTCTCCTCTGTTACCTTCCCCTTGGCGATCGCTCGGG TTTGTGTTAAGTTAATCTGTATGTCCTGTCTGCCAGCGTTCCCTGTACTATAGGCCTGTGTATAGTGTAG GGCAGAGCGTTGACCCACTGGCTAGTGCTTGACTTGGGAATCAGGACAGTACCTGTACAGGCACGGGGAC CCGCTCCGTCCGTGCGCCGCCCTATATTGAGGGCTCCAGCTCTCCCTTGGTTTTTGAAAGGGGTTTATGT ATAAATATATTTTATGCCTTTTTATTACAAGTCTTGTTACTCAATGACTTTTGTCATGGCAGTTTGTTCT ACTTTAGACTGTAAATTATGCATTATAAAGAGTTCATTTAAAGAAAACTACTTGGTACAATAATTATTGT AATTAAGAGATGTAGCCTTTATTAAAATTTTATATTTTTCAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6827741 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6827741 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14737 same embryo
	EMAGE:14739 same embryo
	EMAGE:14738 same embryo
	EurExpress:euxassay_005683 same experiment
	MGI:4823366 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source