EMAGE mouse gene expression spatial database

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EMAGE:14785

Cxcl12 chemokine (C-X-C motif) ligand 12 ( MGI:103556)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005766. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005766_01	euxassay_005766_02	euxassay_005766_03	euxassay_005766_04

euxassay_005766_05	euxassay_005766_06	euxassay_005766_07	euxassay_005766_08	euxassay_005766_09

euxassay_005766_10	euxassay_005766_11	euxassay_005766_12	euxassay_005766_13	euxassay_005766_14

euxassay_005766_15	euxassay_005766_16	euxassay_005766_17	euxassay_005766_18	euxassay_005766_19

euxassay_005766_20	euxassay_005766_21	euxassay_005766_22	euxassay_005766_23	euxassay_005766_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14785_wholemount_strong.wlz

14785_wholemount_moderate.wlz

14785_wholemount_weak.wlz

14785_wholemount_possible.wlz

14785_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14785_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 08 09 10 11 12 14 15 16 17 18 19 20 21 moderate expression: see section 02 03 04 05 06 07 22 23

forearm rest of mesenchyme

strong

regional

strong expression: see section 01 02 moderate expression: see section 03 04

upper arm rest of mesenchyme

moderate

regional

moderate expression: see section 03 04 23 24

hand mesenchyme

moderate

regional

moderate expression: see section 05 06 21 22 24 weak expression: see section 23

interdigital region between hindlimb digits 1 and 2

moderate

regional

moderate expression: see section 07 08 09 22 24 weak expression: see section 23

interdigital region between hindlimb digits 2 and 3

moderate

regional

moderate expression: see section 07 08 09 22 24 weak expression: see section 23

interdigital region between hindlimb digits 3 and 4

moderate

regional

moderate expression: see section 07 08 09 22 24 weak expression: see section 23

interdigital region between hindlimb digits 4 and 5

moderate

regional

moderate expression: see section 07 08 09 22 24 weak expression: see section 23

foot mesenchyme

moderate

regional

moderate expression: see section 21 22

lower leg rest of mesenchyme

strong

regional

strong expression: see section 01 moderate expression: see section 02 03 04 24

upper leg rest of mesenchyme

moderate

regional

moderate expression: see section 04 05 24

axial musculature

moderate

regional

moderate expression: see section 04 20 21 weak expression: see section 03

cranial musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 20 21 22 23 24

physiological umbilical hernia

weak

regional

weak expression: see section 14 15 16

diencephalon meninges

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18

telencephalon meninges

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

hindbrain meninges

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

midbrain meninges

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18

trigeminal v nerve maxillary division

strong

regional

strong expression: see section 06 07 08 20 21 moderate expression: see section 22

extrinsic ocular muscle

moderate

regional

moderate expression: see section 05 06 20 21 weak expression: see section 04 22 23 24

viscerocranium

strong

regional

Expression in the turbinate bone.

aorta

moderate

regional

moderate expression: see section 09 10 11 12 13 14

tongue

weak

regional

weak expression: see section 12 13 15

lower lip

strong

regional

strong expression: see section 13 17 18 moderate expression: see section 09 10 11 12 14 15 19

upper lip

strong

regional

strong expression: see section 13 17 18 19 moderate expression: see section 09 10 11 12 14 15

bladder

weak

regional

weak expression: see section 12 13 14

metanephros

moderate

regional

moderate expression: see section 07 08 09 15 16 17

male reproductive system

moderate

regional

moderate expression: see section 10 14

genital tubercle of male

moderate

regional

moderate expression: see section 12 13 14 weak expression: see section 16

testis

moderate

regional

moderate expression: see section 05 weak expression: see section 06 07 08 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8767
Entity Detected:	Cxcl12, chemokine (C-X-C motif) ligand 12 ( MGI:103556)
Sequence:	sense strand is shown >T8767 ACTCTCGGTCCACCTCGGTGTCCTCTTGCTGTCCAGCTCTGCAGCCTCCGGCGCGCCCTCCCGCCCACGC CATGGACGCCAAGGTCGTCGCCGTGCTGGCCCTGGTGCTGGCCGCGCTCTGCATCAGTGACGGTAAACCA GTCAGCCTGAGCTACCGATGCCCCTGCCGGTTCTTCGAGAGCCACATCGCCAGAGCCAACGTCAAGCATC TGAAAATCCTCAACACTCCAAACTGTGCCCTTCAGATTGTTGCACGGCTGAAGAACAACAACAGACAAGT GTGCATTGACCCGAAATTAAAGTGGATCCAAGAGTACCTGGAGAAAGCTTTAAACAAGGGGCGCAGAGAA GAAAAAGTGGGGAAAAAAAGAAAAGATAGGAAAAAAGAAGCGACAGAAGAAGAGAAAGGCTGCCCAGAAA AGGAAAAACTAGTTCCCCGCTTCCTGCCGATGGACCGCAGTGCGCTCTGCTCTGGCGCTTTGTAACTCGC TCCTCCCTCTTCGGGGGCAGACCCCACACTCCGGGCAGGTGCTCAAACCTGACGGTAAACTCTTCCCTCT TCGGGGGCAGACCCCATACCCCGGGGCGGGTGCTTAGGCTTTCCTGCCCTGGTGGCCACACCAGCTGCTG TATTTATGTGCTTCATAAGGCCCTGCTCTGTCTGCTAAAGCTATGAAGAAAGATGTGCAGAGACTGGGGT GGAGACTAAGCCAAAGAGGAGCTGCCTAGCCTGGCAGCATTGCCCCGAGCTGAGCCCCCTTGGCCAGGAC TTCACAAGGCTCACACCTACAATCCCATGAAGGCCAGGGTGGTCTGCTTAGCCAGGAAAGGGCAAGTGCC TTCCCCTCGGCCACACTGCCCCTTGTGGCCTTCTCGGGACATGTGGTAACTGACTTGCTCTCAGGCCCAC CCGCAGCTTTTCCAAATACCTGCAGCCTTCAGCCCTGCTGCCCTGCCTGTGGGAGCAGCTTTGACTCCAG TCCAGAAGGGTTTCTGCAGACTGTGTTGGGTGAGACGCAGAAAGGATGAAATCTCAGAACACATGTCAGC TGCTTCTCAGGAAATCTTTTCTTTGGACAATTCACTTTAGAGTCTTTAAACGGGTCTCTCGTGGGGAGGA TAGATGTGCTCTGGAACTTTCTGAAGGACCAGCAGCTTCAGGGACTCTTAGTCTGTCCTTCCCCACTTTT GGTCCCAACATCCCTGGGATGGTGTGCTGTCTGGGCACCACGGTCTCCATCCTCACTCCTGAGAGATTTC TGCCTTCTGTGAGTTGGGTTAAAGCTCTGGAATTATCTACTATCCCAATCCACTACCCTCACCTGGCAAT ATTTGTCTGTTTTTGTTTGTTTGTTTGTTTGTTTTTGTCTTTTGCCAGTTTGAATTAGAAGGCAAGGCTC TGATTTTAGTAGTGTTTTGGAAAAGGACTTTTTTCTTCACCTTCCTCTTTGCCTCATGTGTACACACACA CACACATCTTGTACCCCAGACCTCTGGGTATAATTTTCATAATTGGTGCAGAAAGAAGAAATGATCTGAA GATGTGTTAAATGGATTGCAGGGGAAGGAAGGCCCAGGGCCCTGTGTGTCATGCCCTCTTGGGTTCCTAA GTTCTATGTTCCTTAGAGGTTCTAGCATTAAACAGATAAAGCCCTTCATGGTCCTGGCTGAGGAAGAGTC TTGCTAGGGGGATTCAGGGAAGACCCGTGTTACCAGCTCTTACCCTTTATCTGGACAGCTCTCCTACCCT GTATCTTCTCCTCAGATCTGAGGATAGCAGGCTGGACTATTGGTGGGCACCTTTCAAGCCCAGGGCTACT GTTTGTCCTGTGGCAGCCGGCTACAGTCTCGTCTGAGTGGCCTCATCTGGACCCTTCCTGTTATTAATAA AACGCTTCTGGAGGCCAGATCTGTGCTCAAGCCATAGTTCTGCTTAGAAAGGGATGCCCCACCCTTACCG GACACTGGGAAGAACTGTTGGCCCCTAGAAACCAAAGGCCAAACTGAGGCTGCCCTGAGTTGGAAGACCA CTTTCTGAAATGCCCATGGACTCTGCCTCCCAACCATTCGTCTCTCACTCCTAGCAGAGCTGTCTGTGCA GACTGTTTCTTAGGAGGCACAGCAAGCTCCAGGGAACCCTCTGTGCTTATGAAGCTCGTCTGGTGGGCAA CCCCAGCCCACTGGACAGAGTCCTCATGGAAATGCCTGGGAAGCTGATTTCATCTAAGGATGGGTTGAAG TAGGATGTGCTCCTGCGACTTCTCAGGCAGGTGAGAGGGGTAGTCCTTACACTGTCTAGCATAAACGCCT TCCGGAAGGACCTGCAGCTCCAGAGACCACCTCCTGAGCACCAAGACCTCTTCTGGTGGTGTGGAACCAG CCAAGAGATTTCAAGGAAGAGTGATTATTTGATGAATGCTATGGGAATGGCCTCTTCTCTTGGAGTTCTG AGGCCTGGGGATGCCCAGGAACACTGGGCACCTGCTGCTGTTAGGGCCAATGCATAGTCTCAGCACCGGT GTCCTAAGGTTAAGGCGGTGCGCCTTGTCATGTGCTCCTTGTACCATGCCATCTGTGCCAGTGTGTGTCT GCCTCACCCTGTGCTTGACATGTTCACCCATCTTCTCTGCTTCCCGCCACCATCCAGATCCTCAGCGGCC GCCCCGGCTGTGCCCTTCCCTGCTCTCCCGCTCTCTCAGGCCTCGGAAGGAAGATCGGTGGCTGCGAGCT GAACTAAGGAGTAGGGCCTGTGGCTCAGCGCTAGGCCACGCACGCAGCATCCCAGGCATGTGGTGAGAAA CTGCCTTAATGTGTCTCCTCTGTTCTTGTCAACAGGAGGCTCAAGATGTGAGAGGTGTGAGTCAGACGCC CGAGGAACTTACAGGAGGAGCCTAGGTCTGAAGTCAGTGTTAGGGAAGGGCCCATAGCCACTTCCTCTGC TCCTGAGCAGGGCTGAAGCCGTTTGCAAGGGACTTGCTTTGCACAGTTTTGCTGTACTTTCACATTTTAT TATGTAGCAAGATACATGGTGATTTTTTTTTTTTTCATTTAGCCTGATTTTCCAACGTCATTGGTGACAG GCCAAGGCCACTATGTTATTTCCTTTGTTCTGGTATCCTTCCCTTGGAGGACCTTCTCTGAGTAGTGGCT CCCCAGGTTTGTCCTTTGAGCTGAGGCAGGAGGCTCACCCATTCTTCTGAATAGGAACTGGGTGTTCCCA CCCCCCAAGGACTGCAGGGCTTTCCCAAGCTGAGGCAGGAACGTGAGGCCAGGGAAGAGTGAGCTTCACC CTCATCCCACGCTGTCCTCCTCAACCCACCATGCTCATCATTCTGTCTCATCCATCCATCCATCCATCCA TTCATCGCCATGTGTCCGCAAGACTGTCTCCATGACCCTGAAAAAGGACTCTCGAGATGAAATCCTTTAT TCAAATGGGACAGCAAGAAGGAAAAGCCAATGTCTGGTGTCTCTCCCCCCGCCCCTACCCTGCGCGCATC TATGTCTTGTTTGGAATATTGTCTCTTCAACCCCCTGTTCATGTCCTTCTCACTCATGATCGATGTCTTG TCTGTGCACTGTCTCTAACCCAAATGCAAAGGCTGAGTGTGAGGTGATGGCCCCGAGGTCCAGGTTGTAG TCATGGAAAGAGCCCTGCTGTCTCCCTTCTCAGGGGGCCCATTTTAGACACACAAAGCCCAAAGAAAGGT GGTTTGCAACAGTGCTTAGCTCGAGCCTCCATATTTCCATAACTGTTAGCTTAAAACTGTGGGGTTTTAC CTTCCTGGAACCAAATGCATTCTTCTGTTGAGGAGTAACAGGTCTCAATTCTTTTCAATTAATTTTAAAA GTCAATCACTAAGAGCATCGGCTTTGGGCCCTGATGGGCAGGCATTTCCCTGGAAAGGGGGTGAACTACC TACCTCTCCTCAAGACAGCCGAAGGGTGGGATTGGTGCCGCTCTGGGAAGCGTGGCCCCAGGAGTTTTGT CCTCTGCAGTTTTTAATGCAAGTTCACTGCCACTTTGACAAAAGCCCAATTAGAAGCCAGTCTCTAGTTC CTTAAACAAAACAGACAGAGTAAGGAAAGGAAGGAGGGTGGCAGCCAGCTGGCTGGACACTCGAGAAAGA CGGGGAAGTAAGCTACAGAAAGATAGTCTTCAAAAACAGGTGTTTGAGAGTGAATACTCTGTAGAATTGT TAGTGGGGTGTGTGTGGTGGTGGTGGGGGGATTTCTACAAAATAGTCCTTTAAGTTGAGTTTACAGCAGA TGAAAAATCCAACCAGCAAAATTTTGATCAAATTTGAACAAAAACCCAAAAACCTAAAACTGTTGAGCAG GTTGCGATGAGGAGCACAGGGCTAGCTGCAGAGCTGGATCCTCAGGAGGATAGCGAATTATTTTCAACCC TGGAATAGAAACCACACACTGGCTTGCTGTGCACCAGCCACTTTGCATCTAATCCAAGCTTTGAAGGGTG TTGCTTGGGAGGAAACAAATACAGCCTTCCATCTTCACTCCAGTTAGGGATCCTTTCAAAGTCTCCTTCA CAGTGAGGAAAAAGAGAAGGGTAGAAACTTTAGGGAGCCGGATTTGTGTATCAATTCCTCCGCTGACAGT CAGTTTCTAGATGGAGACAGCCTGCTTAAAGCAAATCCGAATTTAAATAGGACATTTACATCGGAAAAGT CTCTCCCTACCTTAATCCCCCATTCTCTTGCTTTCAAAATACAAGCACAGCAGTCCTTGAATGGCTGTTG ACCCAGGGCACCTAGCTGTCCCTGCTGGTCCTGGGGCTGCCAGAATTCCCTTGGGCGCCAAGCAACCTGC CAGGTAGCCAGTCCCTCTGTTACAAGCCTTTGCATCTGGATAGGGAAAGGGGTGGAGACATACAGTCTGC TTTGTGTTGAAACCCAGATTTGTACCCTGTGTTTATACACTGCTGCTGGCTCCCGAGGACAGTGGGACTT TAGCAAGGAAGTGCAGCCGAGGGGTAAAGAGCCCTCTGGTTCATTGCCTGATCGGCTTTGAGAGAGGGTT TGGAGGGCAAGGGGCTGCATTCCTCTGAGGGACTTGGCCTGAGGCCTTTCGGGCCTCTCCAGTGGGTTCT GTTTATCCTCTCATGGGTGATTATCTCAGTGGTGTCACCAGGGGCTTTCTCCCAGAAGTCAGTCATCCCC AGGCCGTGCACCCTTTTCAGCTGGATGAGAGCCAGGGATGCATTCTCTCCAAACAGCTACCCTGGCCCAT TTTAAGGTAATCTCATTCTTCAAAATGTTCCATAGAATCCTCCAAATTCCCCCAGCAGACTTCTACCCTC GCCAAGTTCCCAAAACCCACTCAGCAAAGTTGCCAACCTCGACGGGCTAGCAGTGTCTAAGCAGCGATGG GTTCAGTGTTGTGTGTGGTGAATACTGTATTTTGTTTCAGTTCTGTCTCCCAGATAATGTGAAAACGGTC CAGGAGAAGGCAGCTTCCTATATGCAGCGTGTGCTTTCTTATTCTTATTTTTAATATATGACAGTTATTT GAGAAGCCATTTCTACTTTGAAGTCATTATCGATGAAAGTGATGTATCTTCACCTACCATTTTCCTAATA AAGTTCTGTATTCAAATATAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6406409 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6406409 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14782 same embryo
	EMAGE:14783 same embryo
	EMAGE:14787 same embryo
	EMAGE:14784 same embryo
	EMAGE:14786 same embryo
	EurExpress:euxassay_005766 same experiment
	MGI:4824132 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source