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EMAGE:14789

Cd5l CD5 antigen-like ( MGI:1334419)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14789 EMAGE:14789 EMAGE:14789 EMAGE:14789 EMAGE:14789
"Pseudo-wholemount" of euxassay_005723. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005723_01 euxassay_005723_02 euxassay_005723_03 euxassay_005723_04
EMAGE:14789 EMAGE:14789 EMAGE:14789 EMAGE:14789 EMAGE:14789
euxassay_005723_05 euxassay_005723_06 euxassay_005723_07 euxassay_005723_08 euxassay_005723_09
EMAGE:14789 EMAGE:14789 EMAGE:14789 EMAGE:14789 EMAGE:14789
euxassay_005723_10 euxassay_005723_11 euxassay_005723_12 euxassay_005723_13 euxassay_005723_14
EMAGE:14789 EMAGE:14789 EMAGE:14789 EMAGE:14789 EMAGE:14789
euxassay_005723_15 euxassay_005723_16 euxassay_005723_17 euxassay_005723_18 euxassay_005723_19
EMAGE:14789 EMAGE:14789 EMAGE:14789 EMAGE:14789 EMAGE:14789
euxassay_005723_20 euxassay_005723_21 euxassay_005723_22 euxassay_005723_23 euxassay_005723_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14789Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14789_wholemount_strong.wlz
14789_wholemount_moderate.wlz
14789_wholemount_weak.wlz
14789_wholemount_possible.wlz
14789_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14789_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
liver
strong strong
single cellstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T7233
Entity Detected:Cd5l, CD5 antigen-like ( MGI:1334419)
Sequence:sense strand is shown

>T7233
CTAGTGTTCAAACCAAATACCACTGCCTGAGGCCTACAGCCTGTCTCACCACTCCATTGTCAGCTGCCTG
GGCCATGGCTCCATTGTTCAACTTGATGCTGGCCATCTTGAGCATTTTTGTTGGATCGTGTTTTTCAGAG
TCTCCAACCAAAGTGCAGCTAGTGGGAGGTGCCCACCGCTGTGAAGGGCGAGTGGAGGTGGAACACAATG
GCCAGTGGGGGACTGTGTGTGATGATGGCTGGGACCGGCGTGATGTGGCTGTGGTGTGCCGAGAGCTCAA
TTGTGGAGCAGTCATCCAAACCCCGCGTGGCGCATCATATCAGCCACCAGCATCAGAGCAAAGAGTTCTT
ATTCAAGGGGTTGACTGCAACGGAACGGAAGACACGTTGGCTCAATGTGAGCTAAATTACGATGTTTTTG
ACTGCTCACATGAAGAAGATGCTGGGGCACAGTGTGAGAACCCAGACAGTGACCTCCTCTTCATTCCAGA
GGATGTGCGTCTAGTAGATGGCCCGGGGCACTGCCAGGGTCGAGTGGAGGTGCTCCACCAGTCCCAGTGG
AGCACTGTGTGTAAAGCAGGCTGGAACTTACAGGTCTCAAAGGTGGTGTGCAGGCAGCTCGGGTGTGGGC
GGGCATTACTGACCTACGGAAGCTGCAACAAGAGTACTCAGGGCAAAGGACCCATCTGGATGGGCAAGAT
GTCGTGTTCTGGACAAGAAGCAAACCTTCGGTCTTGCCTTTTGAGTCGTTTGGAGAACAACTGTACCCAT
GGCGAGGACACATGGATGGAATGTGAAGATCCTTTTGAGCTGAAGCTGGTGGGAGGAGACACCCCCTGCT
CTGGGAGGTTGGAGGTGCTGCACAAGGGTTCCTGGGGCTCCGTCTGTGATGACAACTGGGGAGAAAAGGA
GGACCAAGTGGTCTGCAAGCAACTGGGTTGTGGGAAGTCCCTCCATCCATCCCCCAAAACCCGGAAAATC
TATGGGCCTGGGGCAGGCCGCATCTGGCTGGATGACGTCAACTGCTCAGGGAAGGAACAGTCTCTGGAGT
TCTGCCGGCACAGGTTGTGGGGGTACCACGACTGTACCCACAAGGAAGATGTGGAGGTGATCTGCACAGA
CTTTGATGTGTGAATTGGATCCCTGCTTGTTCAGTGGGCCCTCATTCTCCCAGTGGTTACATCAGGCTGT
GGGCTTTAGACACCTTTCCCTCAGCCTCGAAAGAGTCTGAACATTGTGTTCCTATCTTGATCTCAAGGCT
ACACGCCCCCATAATCACCTCAAGACATGAGCTGCTGAGCTCCCTTGCTGACCTTTCCAGCTGCCCTAGG
CTCACTGTTCACTCCTTGGTGAAAAGCCCCCACCTTTACTGTCTCTCCCCAGCCTGCCTGCAACTCTTGG
GCCTGCCAGAGTGAGCAGCTGTACAGGCCAGGAGTAAGACACGGCCTGTCTGTGAACACCACTGAGGATG
TGACAACATGAGGAACACTTGAGAGGGAATGTGGGTAGACAGATTCTTGGAGGCGGGAGAGATAATACAA
TTGTTTAAATGCTTTTTAAACTTTGTAACAAGTGAAGTGATCATAATAATAACACTTCACTACTCTGCTT
CTCTCAGAGAAAGCAGCAGGGTGGTTTCCTGCAGCCCTCAAATGTTACCTGTTGAGTTCTAGATGTCTAC
CCCAAACCTCCATGTTTAAAGTTTGATGTCTAATGCAACAGTATTCTGAGGTGGGGCCTTAGGGATCTAA
CTGCATCATGTGGTTTGATCCCTCAGTCTTTATGAGTGGATTAATCACTGGTGAATCCCCTGGGAGATGG
TAGAGACTTGAGGAGTTGGGACCTAGTTAGAAGAAGAAGGTCGTTGGAGTGTGCCTTTGGAAGGGGCTGT
TTTGTCCACAGCTTGACCACCTCCCTGTGTAGATGGGTCGCTTGCATATCTTCTCTACTGTGTGTGAATT
ATGCTACAATAAACATGAGTGCTCAAGTAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:4161650 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4161650 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14790 same embryo
 EMAGE:14788 same embryo
 EMAGE:14792 same embryo
 EMAGE:14791 same embryo
 EurExpress:euxassay_005723 same experiment
 MGI:4823739 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS