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EMAGE:14799

Rere arginine glutamic acid dipeptide (RE) repeats ( MGI:2683486)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14799 EMAGE:14799 EMAGE:14799 EMAGE:14799 EMAGE:14799
"Pseudo-wholemount" of euxassay_005784. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005784_01 euxassay_005784_02 euxassay_005784_03 euxassay_005784_04
EMAGE:14799 EMAGE:14799 EMAGE:14799 EMAGE:14799 EMAGE:14799
euxassay_005784_05 euxassay_005784_06 euxassay_005784_07 euxassay_005784_08 euxassay_005784_09
EMAGE:14799 EMAGE:14799 EMAGE:14799 EMAGE:14799 EMAGE:14799
euxassay_005784_10 euxassay_005784_11 euxassay_005784_12 euxassay_005784_13 euxassay_005784_14
EMAGE:14799 EMAGE:14799 EMAGE:14799 EMAGE:14799 EMAGE:14799
euxassay_005784_15 euxassay_005784_16 euxassay_005784_17 euxassay_005784_18 euxassay_005784_19
EMAGE:14799 EMAGE:14799 EMAGE:14799
euxassay_005784_20 euxassay_005784_21 euxassay_005784_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14799Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14799_wholemount_strong.wlz
14799_wholemount_moderate.wlz
14799_wholemount_weak.wlz
14799_wholemount_possible.wlz
14799_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14799_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
brain
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22
adenohypophysis
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13 14
vibrissa
weak weak
regionalweak expression: see section 05 06 07
facial vii ganglion
moderate moderate
regionalmoderate expression: see section 04 17 18 19
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 04 05 15
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08 16 17 18 19 20
vagus x ganglion
moderate moderate
regionalmoderate expression: see section 05 15
trigeminal v nerve
moderate moderate
regionalmoderate expression: see section 15
spinal cord
moderate moderate
regionalmoderate expression: see section 05 06 07 08 09 10 11 12 13 14 15
cervico-thoracic ganglion
moderate moderate
regionalmoderate expression: see section 06 12
cervical ganglion
moderate moderate
regionalmoderate expression: see section 05 06 13 14
thoracic ganglion
moderate moderate
regionalmoderate expression: see section 10 11 12
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 04 05 06 07 11 12 13 14
neural retina
moderate moderate
regionalmoderate expression: see section 02 03 22
stomach
weak weak
regionalweak expression: see section 02 03 04
midgut
weak weak
regionalweak expression: see section 11 12 13 14 15 16
metanephros
moderate moderate
regionalmoderate expression: see section 06 07 08 14 15 16 17
lung
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35526
Entity Detected:Rere, arginine glutamic acid dipeptide (RE) repeats ( MGI:2683486)
Sequence:sense strand is shown

>T35526
CTAGTTTTAAGCAGGCAGGAGCTTGGGACTAGGTTTGGTCACAGCAGCGAGCATCCAAGGGTTGGCAGGG
ACAGTGTTACAGGCTCTTTATTTTGCTGCTTTGATTTTGCTTCTGTCTGCCCCTCACATGTGCAGTGGCA
GGCGTGTTTGAGCGAGCCTCCTTCCTTATTCATAGGGATTCAGTGTTGACTGTTCCCTGGTGTCCCCGCT
GTCCTGTGTCGTCTGGTATAGAAAGCGCATGTTTCTGTATCGGTGTCTGATGATAAGTTTCCTTGGAGTT
TTATGCACATGTTTGATTGTTCTAGATAAATTGTCATAAACATTCTAGGAAGTTCAAGAAAATGATGCAG
TACCCAGCACATGAAAGACCTACAACATGTGCTTGCCAAAGGAATGTTAGAATCTGCTCATGTGTGTGTC
ACCATGCCAAGCCATGCCAGCCACAGACTGAGGCTTCCGGGTGAGGAACACCCTGGGGCTACTATGTTTG
TAGGTCTCATGTGCTCAGGGCTATGAAGTAGGCTGTGCAGGTGACAGCACCCTGCTAGGAAGCTGTGTGT
GTCCTGCCTTTCCTCCTGGAATATGTACGGATGGAAGTCTTGGTTGAAGCAGGTTCCCCTGGGAAGTGCT
CTCAGCAGGCCTTGCGGAGCAGAGCAGAGCTGGGCAGAGCTGACTCCTGCCCCGCTGTCCCTGATGGAAC
CTTCCTTCCAGAGCTGTGGAAGAGATGTTTGTGACCCCTCCCTTGGACTCTTGGGGGCCAATCCCACTCT
TGAGTGCAATGGTTGCATTTAGAATCAGCTAGC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 78393. Forward Primer - name:078393_F_cDNA_Rere, sequence:CTAGTTTTAAGCAGGCAGGAGC; Reverse Primer - name:078393_N_SP6_cDNA_Rere, sequence:GCTAGCTGATTCTAAATGCAACC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14801 same embryo
 EMAGE:14800 same embryo
 EurExpress:euxassay_005784 same experiment
 MGI:4827698 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS