EMAGE mouse gene expression spatial database

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EMAGE:14910

Thsd4 thrombospondin, type I, domain containing 4 ( MGI:2672033)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007983. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007983_01	euxassay_007983_02	euxassay_007983_03	euxassay_007983_04

euxassay_007983_05	euxassay_007983_06	euxassay_007983_07	euxassay_007983_08	euxassay_007983_09

euxassay_007983_10	euxassay_007983_11	euxassay_007983_12	euxassay_007983_13	euxassay_007983_14

euxassay_007983_15	euxassay_007983_16	euxassay_007983_17	euxassay_007983_18	euxassay_007983_19

euxassay_007983_20	euxassay_007983_21	euxassay_007983_22	euxassay_007983_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14910_wholemount_strong.wlz

14910_wholemount_moderate.wlz

14910_wholemount_weak.wlz

14910_wholemount_possible.wlz

14910_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14910_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

weak

regional

weak expression: see section 04 05 06 07 08 09 10 11 13 14 15 16 17 18 19

hindlimb digit 1 metatarsal

weak

regional

weak expression: see section 06 07 22

hindlimb digit 2 metatarsal

weak

regional

weak expression: see section 05 06 07 21 22

hindlimb digit 3 metatarsal

weak

regional

weak expression: see section 05 06 07 22

hindlimb digit 4 metatarsal

weak

regional

weak expression: see section 05 06 07 22

hindlimb digit 5 metatarsal

weak

regional

weak expression: see section 06 07 22

tongue

weak

regional

weak expression: see section 11 12 13 14 15 16

lower lip

weak

regional

weak expression: see section 07 08

upper lip

weak

regional

weak expression: see section 07 08

cricoid cartilage

moderate

regional

moderate expression: see section 11 12

tail skeleton

weak

regional

weak expression: see section 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35980
Entity Detected:	Thsd4, thrombospondin, type I, domain containing 4 ( MGI:2672033)
Sequence:	sense strand is shown >T35980 GGTCTTATTTGAGCCTGTGGACTGTCTGGAGTTTCTATCTAGTGGTCTCGCATCGCTCCCCTCCACTGTG ACTCATGACTCTTTCTTCCCACAAGCCTTGACCATCGAGACTAACTTTAATGCTGCAGGTCTGAGAGAAG GAGCTAGGAAGCCCTCCGTAGCTCTGCTGACTCACTCTGCCCAACTGTAATTCCAAAGGCCCAGGCCTGA AGGGACTCAGTGTTTATTGAAAGGTGAACCATCCTGGGGTGCCAGGGACTGGGGAGAGACAATTAGGAGA AGTCTTCTTTCTTTCTTAAAAATTGTAAACTTACAGAAAGGATCTTATTTCGGGTCCTGGGCATGTTTCC CTTAAAGAAAGCACATACTCTTAGCATACGATCTCTCCATGAAGCAGCCTGCACTATGACCGACCGCTCA CGGCTGTGGGGATGGTATAAGCTGTCCGGTCTGCACTTCATGAGGATTCCAATGGCCTCCACCTTTTGCA AGTGTCCCCTCAGCCTTTGTCTCTGCCTGAGAAGCGAAGCACTGCTTCCATCCGTAAGCAGCCCACCTCT CCTGTGGAGCAGGAGACACACAGCCCAGCAGAGACCGCACTCGCCATGCTAAGCACAGAGAGAAG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 93174. Forward Primer - name:093174_F_cDNA_B230114P05Rik, sequence:GGTCTTATTTGAGCCTGTGGAC; Reverse Primer - name:093174_N_SP6_cDNA_B230114P05Rik, sequence:CTTCTCTCTGTGCTTAGCATGGC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14915 same embryo
	EMAGE:14912 same embryo
	EMAGE:14911 same embryo
	EMAGE:14914 same embryo
	EMAGE:14913 same embryo
	EurExpress:euxassay_007983 same experiment
	MGI:4828705 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source