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EMAGE:14968

Bmp5 bone morphogenetic protein 5 ( MGI:88181)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14968 EMAGE:14968 EMAGE:14968 EMAGE:14968 EMAGE:14968
"Pseudo-wholemount" of euxassay_008006. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008006_01 euxassay_008006_02 euxassay_008006_03 euxassay_008006_04
EMAGE:14968 EMAGE:14968 EMAGE:14968 EMAGE:14968 EMAGE:14968
euxassay_008006_05 euxassay_008006_06 euxassay_008006_07 euxassay_008006_08 euxassay_008006_09
EMAGE:14968 EMAGE:14968 EMAGE:14968 EMAGE:14968 EMAGE:14968
euxassay_008006_10 euxassay_008006_11 euxassay_008006_12 euxassay_008006_13 euxassay_008006_14
EMAGE:14968 EMAGE:14968 EMAGE:14968 EMAGE:14968 EMAGE:14968
euxassay_008006_15 euxassay_008006_16 euxassay_008006_17 euxassay_008006_18 euxassay_008006_19
EMAGE:14968 EMAGE:14968 EMAGE:14968
euxassay_008006_20 euxassay_008006_21 euxassay_008006_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14968Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14968_wholemount_strong.wlz
14968_wholemount_moderate.wlz
14968_wholemount_weak.wlz
14968_wholemount_possible.wlz
14968_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14968_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
rib
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 10 11 13 14 15 16 weak expression: see section 17 18 19 20
humerus
moderate moderate
regionalmoderate expression: see section 03 04 18 19
hindlimb digit 1 skin
moderate moderate
regionalmoderate expression: see section 02
hindlimb digit 2 skin
moderate moderate
regionalmoderate expression: see section 01 17 weak expression: see section 02
hindlimb digit 3 skin
moderate moderate
regionalmoderate expression: see section 01 15 16
hindlimb digit 4 skin
moderate moderate
regionalmoderate expression: see section 02 15
tarsus
moderate moderate
regionalmoderate expression: see section 01 18 weak expression: see section 02 03
fibula
weak weak
regionalweak expression: see section 20 21
tibia
weak weak
regionalweak expression: see section 20 21
femur
moderate moderate
regionalmoderate expression: see section 04 05 20
otic capsule
moderate moderate
regionalmoderate expression: see section 07 08 15 16
pharyngo-tympanic tube
moderate moderate
regionalmoderate expression: see section 01 02 03 21 22
nasal septum
moderate moderate
regionalmoderate expression: see section 10 11 12 13 14 15
hyoid
moderate moderate
regionalmoderate expression: see section 15 weak expression: see section 10 14
mandible
weak weak
regionalweak expression: see section 03 04 19 20
maxilla
moderate moderate
regionalmoderate expression: see section 02 weak expression: see section 03 04 19 20 21 22
cricoid cartilage
moderate moderate
regionalmoderate expression: see section 10 11 13
thyroid cartilage
moderate moderate
regionalmoderate expression: see section 11 12 13
temporal bone petrous part
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 17 18 19 20 21 22
viscerocranium
moderate moderate
regionalExpression in the turbinate bone.
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36054
Entity Detected:Bmp5, bone morphogenetic protein 5 ( MGI:88181)
Sequence:sense strand is shown

>T36054
ATAGAAGGCTACGGAACCATGAGAGACGGGAAATACAGAGGGAAATTCTGTCTATTTTGGGTTTGCCTCA
CAGACCCAGGCCCTTTTCACCAGGGAAACAAGCATCTTCTGCCCCCCTCTTTATGCTGGATCTCTACAAT
GCCATGGCTAGCGAAGACAACCCTGAGGAGTATTTGGTGAGGGTGTCCCTGGCTGGAGAGGCCAAAGAGA
CAAGAAAGGGATACCCAGCCTCTCCCAATGGGTATGCGCATCGTCTGCACTTACCACCAAGGACTCCTCT
GACTACCCAGAGCCCACCTCTTGCCAGCCTACATGATACCAACTTTCTGAATGATGCTGACATGGTCATG
AGCTTTGTCAACTTAGTTGAAAGAGACAAGGACTTTTCTCATCAGAGGAGGCATTACAAAGAGTTTCGGT
TTGATCTGACCCAGATTCCACATGGAGAAGCAGTGACAGCAGCAGAATTCCGGATTTATAAGGACAAGGG
AAACCATCGATTTGAAAATGAAACAATTAAGATCAGCATATATCAGATCATCAAGGAATACACAAACAGG
GATGCAGATCTTTTTCTGCTAGATACGAGGAAGACACAAGCTTTAGATGTGGGCTGGCTTGTCTTTGATA
TCACTGTGACCAGCAACCACTGGGTGATTAATCCAC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 97424. Forward Primer - name:097424_F_cDNA_Bmp5, sequence:ATAGAAGGCTACGGAACCATGA; Reverse Primer - name:097424_N_SP6_cDNA_Bmp5, sequence:GTGGATTAATCACCCAGTGGT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14970 same embryo
 EMAGE:14969 same embryo
 EMAGE:14971 same embryo
 EurExpress:euxassay_008006 same experiment
 MGI:4823486 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS