EMAGE mouse gene expression spatial database

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EMAGE:15033

Fst follistatin ( MGI:95586)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010196. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010196_01	euxassay_010196_02	euxassay_010196_03	euxassay_010196_04

euxassay_010196_05	euxassay_010196_06	euxassay_010196_07	euxassay_010196_08	euxassay_010196_09

euxassay_010196_10	euxassay_010196_11	euxassay_010196_12	euxassay_010196_13	euxassay_010196_14

euxassay_010196_15	euxassay_010196_16	euxassay_010196_17	euxassay_010196_18	euxassay_010196_19

euxassay_010196_20	euxassay_010196_21	euxassay_010196_22	euxassay_010196_23	euxassay_010196_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15033_wholemount_strong.wlz

15033_wholemount_moderate.wlz

15033_wholemount_weak.wlz

15033_wholemount_possible.wlz

15033_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15033_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper arm muscle

strong

regional

strong expression: see section 01 02 03 04 22 23 24

upper leg muscle

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 17 18 19 20 21 22 23 24

diaphragm

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 15 16 17 18 19 20 21 22 23 24

forearm rest of mesenchyme

strong

regional

strong expression: see section 01 02 23 24

hand

strong

regional

strong expression: see section 02 03 04 05 06 07 22 23 24

foot

strong

regional

strong expression: see section 07 08 09 10 11 21 22 23 24

lower leg rest of mesenchyme

strong

regional

strong expression: see section 03

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

telencephalon mantle layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 20 21 22 23

olfactory cortex mantle layer

strong

regional

strong expression: see section 11 12 13 14 16 17 18 19 20

rest of cerebellum mantle layer

strong

regional

strong expression: see section 08 09 10

rest of cerebellum marginal layer

strong

regional

strong expression: see section 01 02 03 14 15 16 17

pons mantle layer

strong

regional

strong expression: see section 09

cochlea

strong

regional

strong expression: see section 05 06 07 15 16 17

cornea

strong

regional

strong expression: see section 02 03 04 05 22 23 24

eye skeletal muscle

strong

regional

strong expression: see section 03 04 05 06 07 08 19 20 21 22 23

lens

strong

regional

strong expression: see section 01

naris

strong

regional

strong expression: see section 14 15 16 17 18

nasal cavity

strong

regional

strong expression: see section 10 11 12 13 14 17 18 19 20

tongue muscle

strong

regional

strong expression: see section 11 12 13 14 15 16 17 18

stomach

strong

regional

strong expression: see section 01 02 03 04 05 06 07

midgut loop

strong

regional

strong expression: see section 14 15 16 17 18 19

lower jaw incisor

strong

regional

strong expression: see section 10 11 12 13 16 17

lower jaw molar

strong

regional

strong expression: see section 07 08 19 20

upper jaw incisor

strong

regional

strong expression: see section 13 14 18

upper jaw molar

strong

regional

strong expression: see section 07 08 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36396
Entity Detected:	Fst, follistatin ( MGI:95586)
Sequence:	sense strand is shown >T36396 CTACTCTGCCAGTTCATGGAGGACCGCAGCGCCCAGGCTGGGAATTGCTGGCTCCGCCAAGCAAAGAACG GCCGCTGCCAGGTCCTGTATAAGACAGAACTGAGCAAGGAAGAGTGTTGCAGCACCGGCCGGCTGAGCAC CTCATGGACCGAGGAGGATGTGAACGACAATACTCTCTTCAAGTGGATGATTTTCAACGGGGGCGCCCCC AACTGCATCCCTTGTAAAGAAACGTGTGAGAACGTGGACTGCGGACCTGGGAAAAAATGTCGAATGAACA AGAAGAATAAACCCCGCTGCGTCTGTGCCCCAGACTGTTCCAACATCACCTGGAAGGGCCCAGTGTGTGG GCTGGATGGGAAAACCTACCGCAACGAATGTGCACTCCTCAAGGCCAGATGCAAAGAGCAGCCGGAACTA GAAGTACAGTACCAGGGCAAATGTAAAAAGACTTGTAGGGATGTTTTTTGTCCAGGCAGCTCCACTTGTG TGGTGGATCAGACCAATAATGCCTACTGTGTGACCTGTAATCGGATTTGCCCAGAGCCCTCCTCTTCTGA ACAGTACCTTTGTGGAAATGATGGAGTGACTTACTCCAGCGCCTGCCACCTGAGAAAGGCCACCTGCTTG CTGGGCAGATCCATTGGATTAGCCTATGAGGGAAAGTGTATCACAAAGTCCTGTGAAGATATCCAGTGTG GCGGCGGGAAAAAATGCCTATGGGATTCCAAGGTTGGCAGAGGT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 97739. Forward Primer - name:097739_F_cDNA_Fst, sequence:CTACTCTGCCAGTTCATGGAGG; Reverse Primer - name:097739_N_SP6_cDNA_Fst, sequence:ACCTCTGCCAACCTTGGAATC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15036 same embryo
	EMAGE:15037 same embryo
	EMAGE:15038 same embryo
	EMAGE:15035 same embryo
	EMAGE:15034 same embryo
	EurExpress:euxassay_010196 same experiment
	MGI:4824938 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source