EMAGE mouse gene expression spatial database

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EMAGE:15226

Slc35b2 solute carrier family 35, member B2 ( MGI:1921086)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010411. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010411_01	euxassay_010411_02	euxassay_010411_03	euxassay_010411_04

euxassay_010411_05	euxassay_010411_06	euxassay_010411_07	euxassay_010411_08	euxassay_010411_09

euxassay_010411_10	euxassay_010411_11	euxassay_010411_12	euxassay_010411_13	euxassay_010411_14

euxassay_010411_15	euxassay_010411_16	euxassay_010411_17	euxassay_010411_18	euxassay_010411_19

euxassay_010411_20	euxassay_010411_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15226_wholemount_strong.wlz

15226_wholemount_moderate.wlz

15226_wholemount_weak.wlz

15226_wholemount_possible.wlz

15226_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15226_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20 21

humerus

weak

regional

weak expression: see section 01 02 03 04 05 19 20 21

submandibular gland primordium

weak

regional

weak expression: see section 07 08 09 15 16

otic capsule

weak

regional

weak expression: see section 07 08 09 10 15 16 17

mandible

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 16 17 18 19 20 weak expression: see section 10 11 12 14 15

maxilla

moderate

regional

moderate expression: see section 05 06 07 08 09 16 17 18 19 20 21 weak expression: see section 10 11 12

basioccipital bone

moderate

regional

moderate expression: see section 02 12 21

basisphenoid bone

moderate

regional

moderate expression: see section 02 12 21

exoccipital bone

moderate

regional

moderate expression: see section 02 03 04 05 06 17 18 19 20 21

temporal bone petrous part

moderate

regional

moderate expression: see section 01 02 03 04 05 06 18 19 20 21

orbito-sphenoid

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 17 18 19 20 21

viscerocranium

moderate

regional

Expression in the turbinate bone.

clavicle

weak

regional

weak expression: see section 07 08 09 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30243
Entity Detected:	Slc35b2, solute carrier family 35, member B2 ( MGI:1921086)
Sequence:	sense strand is shown >T30243 AGGCCTCCAAGGTGATCCCTGTCATGATGATGGGAAAGCTGGTGTCCCGGCGCAGCTATGAACACTGGGA ATACCTGACTGCCGGCCTCATCTCCATTGGAGTGAGCATGTTTCTTCTATCCAGTGGACCAGAGCCTAGA AGCTCTCCAGCCACCACACTCTCTGGCTTGGTCCTACTGGCAGGCTATATTGCTTTCGACAGCTTCACCT CAAATTGGCAGGATGCCCTGTTTGCCTATAAGATGTCATCGGTGCAGATGATGTTTGGGGTCAATTTATT CTCCTGTCTTTTCACAGTAGGCTCACTACTGGAACAGGGGGCCCTACTGGAGGGGGCACGCTTCATGGGG CGGCACAGTGAGTTTGCGCTCCATGCTCTCCTCCTCTCCATCTGCTCCGCCTTTGGGCAGCTCTTCATCT TCTACACCATTGGACAATTTGGAGCTGCTGTCTTCACTATCATCATGACTCTACGCCAGGCTATTGCCAT CCTCCTCTCCTGCCTCCTCTATGGCCATACTGTCACTGTGGTGGGGGGACTGGGAGTAGCTGTGGTCTTC ACTGCCCTCCTACTCAGAGTCTATGCCCGGGGCCGGAAGCAGCGGGGAAAGAAGGCTGTGCCCACTGAGC CCCCAGTACAGAAGGTGTGAGCAGTGCAGTAAAGACCCTCATCTTCTGAGGCACTGGCTCAGTATCAGCA TACAGCAGAGGATTGGAGCCCTGGAGGCAGCCTCTTTTGCCTTAAAAGCCCCCACTTCATGGAAATGACA GCTGTGGGTGTTTGGTTAGAGGTGACCCAGAGCTCCTCCCCCAATCTCTGAAATCTTGCTGGTGGCCAAG CAAACCAGCACCAGGGCTTTGCTCATAGCACGCACCCTTGAGGCTACCAGGCACCAGCTGGGAAGAGAAT TGTTTACAGGTCATGCAGTTCCCCTAGGGGCCAGTGAGAATGGT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5137689), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 26246. Forward Primer - name:026246_F_IRAV63-66_E12_Slc35b1, sequence:AGGCCTCCAAGGTGATCC; Reverse Primer - name:026246_R_SP6_IRAV63-66_E12_Slc35b1, sequence:CACCATTCTCACTGGCCC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15230 same embryo
	EMAGE:15225 same embryo
	EMAGE:15229 same embryo
	EMAGE:15228 same embryo
	EMAGE:15227 same embryo
	EurExpress:euxassay_010411 same experiment
	MGI:4828198 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source