EMAGE mouse gene expression spatial database

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EMAGE:15227

Ngfr nerve growth factor receptor (TNFR superfamily, member 16) ( MGI:97323)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010406. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010406_01	euxassay_010406_02	euxassay_010406_03	euxassay_010406_04

euxassay_010406_05	euxassay_010406_06	euxassay_010406_07	euxassay_010406_08	euxassay_010406_09

euxassay_010406_10	euxassay_010406_11	euxassay_010406_12	euxassay_010406_13	euxassay_010406_14

euxassay_010406_15	euxassay_010406_16	euxassay_010406_17	euxassay_010406_18	euxassay_010406_19

euxassay_010406_20	euxassay_010406_21	euxassay_010406_22	euxassay_010406_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15227_wholemount_strong.wlz

15227_wholemount_moderate.wlz

15227_wholemount_weak.wlz

15227_wholemount_possible.wlz

15227_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15227_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 18 19 20 21 22 23 weak expression: see section 08 09 17

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

vibrissa

moderate

regional

moderate expression: see section 04 05 06 07 08 18 19 20 21 22 23

diencephalon meninges

moderate

regional

moderate expression: see section 17 18 weak expression: see section 08 09 10 11 12 13 14 15 16

telencephalon meninges

moderate

regional

moderate expression: see section 17 18 22 23 weak expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 19 20 21

4th ventricle

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 18 19

medulla oblongata alar plate

moderate

single cell

moderate expression: see section 09

medulla oblongata basal plate

moderate

single cell

moderate expression: see section 09 14 15

medulla oblongata basal plate mantle layer

strong

single cell

strong expression: see section 09 15 moderate expression: see section 08 10 11 12 13 14

hindbrain meninges

moderate

regional

moderate expression: see section 17 18 weak expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 19 20

pons mantle layer

moderate

regional

moderate expression: see section 08 17

midbrain meninges

moderate

regional

moderate expression: see section 17 18 weak expression: see section 06 07 08 09 10 11 12 13 14 15 16 19

facial vii ganglion

strong

regional

strong expression: see section 04 05 06 19 20

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 07 08 16 17 18

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 08 17 18 19 20 21 22

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 07 08 16 17 18

ventral grey horn

strong

regional

strong expression: see section 09 10 11 12 13 14 15

spinal cord meninges

weak

regional

weak expression: see section 09 10 11 12 13 14

dorsal root ganglion

strong

regional

strong expression: see section 07 08 09 10 11 12 14 15 16

neural retina

moderate

regional

moderate expression: see section 02 03 04 23

tongue

moderate

regional

moderate expression: see section 13 14 weak expression: see section 11 12 15 16

tail mesenchyme

moderate

regional

moderate expression: see section 12 13 14 weak expression: see section 15 16 17 18 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30242
Entity Detected:	Ngfr, nerve growth factor receptor (TNFR superfamily, member 16) ( MGI:97323)
Sequence:	sense strand is shown >T30242 AGCGGCATCTCTGTGGACAGCCAGAGCCTGCACGACCAGCAGACCCACACACAGACTGCCTCAGGCCAAG CCCTCAAGGGTGATGGCAACCTCTACAGTAGCCTGCCCCTGACCAAGCGTGAGGAGGTCGAGAAGCTGCT CAATGGTGACACCTGGCGACATCTGGCAGGCGAGCTGGGCTACCAGCCGGAGCATATAGACTCCTTTACC CACGAGGCCTGCCCAGTCCGAGCCCTGCTGGCCAGCTGGGGTGCCCAGGACAGCGCGACGCTCGATGCCC TTTTAGCCGCCCTGCGACGCATCCAGAGAGCTGACATTGTGGAGAGCCTGTGCAGCGAGTCCACTGCCAC GTCCCCTGTGTGAGCTCACCGACTGGGAGCCCCTGTCCTGTCCCACATTCCGACCGCTGATGTTCTAGCC CCCACAGAGCCGCCCCCCTCTCCCTTGGGGTGGCCCAACAGTCAGAACCGAGCATCTCTGTGTAGGGCCT CTGTGTCCCGCTCCCCAAACCACAGCCCTGTTGCTGCTCCTGAGGGGACCTTTGCTTCTGACCACACTCT CTCTCCGGCGAGAGATAGGACCACCCCAAGCCTGTCTTGCCCCGTGACCATCTCAGGCCTTTCCTTTCTT TCTACACATTAACTGTGTCAGATCTAGGGGTCTGACACTAGAGAAGGTAACAGGGGCACCCCTAAGACTC AGGGGGTACTAATGAACCAGAGCCATGGACTCCACACTGTGAACTGGAGAACAGGGGGGGCATTGTGGTA GGCCAGACCTTCCTCAACCCCTCCCTTCTC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5367638), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 27375. Forward Primer - name:027375_F_IRAV63-66_D19_Ngfr, sequence:AGCGGCATCTCTGTGGAC; Reverse Primer - name:027375_R_SP6_IRAV63-66_D19_Ngfr, sequence:GGAGAAGGGAGGGGTTGA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15230 same embryo
	EMAGE:15225 same embryo
	EMAGE:15229 same embryo
	EMAGE:15228 same embryo
	EMAGE:15226 same embryo
	EurExpress:euxassay_010406 same experiment
	MGI:4826699 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source