EMAGE mouse gene expression spatial database

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EMAGE:15236

Creb3l1 cAMP responsive element binding protein 3-like 1 ( MGI:1347062)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010439. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010439_01	euxassay_010439_02	euxassay_010439_03	euxassay_010439_04

euxassay_010439_05	euxassay_010439_06	euxassay_010439_07	euxassay_010439_08	euxassay_010439_09

euxassay_010439_10	euxassay_010439_11	euxassay_010439_12	euxassay_010439_13	euxassay_010439_14

euxassay_010439_15	euxassay_010439_16	euxassay_010439_17	euxassay_010439_18	euxassay_010439_19

euxassay_010439_20	euxassay_010439_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15236_wholemount_strong.wlz

15236_wholemount_moderate.wlz

15236_wholemount_weak.wlz

15236_wholemount_possible.wlz

15236_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15236_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 01 02 03 04 05 06 07 15 16 17 18 19 20 21

humerus

strong

regional

strong expression: see section 01 02 20 21

hip

strong

regional

strong expression: see section 11 12 18

fibula

strong

regional

strong expression: see section 03 04

tibia

strong

regional

strong expression: see section 04 05

femur

strong

regional

strong expression: see section 05 06 07

stomach

weak

regional

weak expression: see section 03 04 05 06 07 08 09 10

rectum

strong

regional

strong expression: see section 15

mandible

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 13 14 15 16 17 18 19 20 21

maxilla

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 14 15 16 17 18 19 20 21

bladder

moderate

regional

moderate expression: see section 12 13 14 15 16

frontal bone primordium

strong

regional

strong expression: see section 01 02 03

orbito-sphenoid

strong

regional

strong expression: see section 03 04 05 06 21

clavicle

strong

regional

strong expression: see section 01 02 03 04 05 06 07 14 15 16 17

scapula

strong

regional

strong expression: see section 01 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30199
Entity Detected:	Creb3l1, cAMP responsive element binding protein 3-like 1 ( MGI:1347062)
Sequence:	sense strand is shown >T30199 CAGATGCCTCCAACACCCCCCAGCAGCCATGGCAGTGACAGTGACGGCTCCCAGAGTCCCCGCTCTCTTC CCCCCTCCAGCCCTGTCCGGCCCATGGCCCGCTCCTCCACGGCCATTTCCACCTCTCCGCTCCTCACTGC CCCTCACAAACTGCAGGGGACATCAGGGCCACTGCTCTTGACAGAAGAGGAGAAGCGGACCTTGATCGCG GAGGGTTACCCTATCCCCACCAAGCTCCCCCTCACCAAGGCTGAGGAGAAGGCCTTGAAGAGAGTACGCA GGAAAATTAAGAACAAGATTTCTGCCCAGGAGAGCCGCCGCAAGAAGAAGGAGTATGTGGAATGTCTAGA AAAGAAGGTGGAGACATATACATCAGAGAACAATGAGCTGTGGAAGAAAGTGGAAACCCTAGAGACTGCC AACAGGACCCTGCTCCAGCAGCTGCAGAAACTCCAGACTCTGGTCACCAGCAAGATCTCCAGACCGTACA AGATGGCAGCCACGCAGACCGGCACCTGCCTCATGGTGGCAGCCTTGTGCTTCGTTCTGGTGCTGGGCTC CCTTGTGCCCTGCCTTCCTGCATTCTCTTCCGGCTCAATGACTGTGAAAGAAGACCCTATCGCAGCTGAC AGTGTCTATGCAGCCAGTCAGATGCCTTCCCGAAGCCTACTGTTCTACGATGATGGGGCAGGCTCATGGG AAGATGGCCGAGGTGCTCTACTGCCTGTGGAGCCCCCAGAAGGCTGGGAGCTCAAACCCGGGGGTCCAGC AGAGCAGAGGCCCCAGGACCACCTCCGACATGACCGTGCAGACAGC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4921584), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 19995. Forward Primer - name:019995_F_IRAV51-54_A18_Creb3l1, sequence:CAGATGCCTCCAACACCC; Reverse Primer - name:019995_R_SP6_IRAV51-54_A18_Creb3l1, sequence:TGCTGTCTGCACGGTCAT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15240 same embryo
	EMAGE:15239 same embryo
	EMAGE:15237 same embryo
	EMAGE:15238 same embryo
	EurExpress:euxassay_010439 same experiment
	MGI:4824045 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source