EMAGE mouse gene expression spatial database

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EMAGE:15326

Camk2d calcium/calmodulin-dependent protein kinase II, delta ( MGI:1341265)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010500. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010500_01	euxassay_010500_02	euxassay_010500_03	euxassay_010500_04

euxassay_010500_05	euxassay_010500_06	euxassay_010500_07	euxassay_010500_08	euxassay_010500_09

euxassay_010500_10	euxassay_010500_11	euxassay_010500_12	euxassay_010500_13	euxassay_010500_14

euxassay_010500_15	euxassay_010500_16	euxassay_010500_17	euxassay_010500_18	euxassay_010500_19

euxassay_010500_20	euxassay_010500_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15326_wholemount_strong.wlz

15326_wholemount_moderate.wlz

15326_wholemount_weak.wlz

15326_wholemount_possible.wlz

15326_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15326_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 moderate expression: see section 09 10 11 12 13 14 15 16 17 18

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 15 moderate expression: see section 07 08 09 10 13 14

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 10 11 12

telencephalon mantle layer

strong

regional

strong expression: see section 01 02 03 04 05 06 15 16 17 18 19 20 21 moderate expression: see section 11 14

telencephalon ventricular layer

strong

regional

strong expression: see section 01 02 03 04 05 17 18 19 20

medulla oblongata alar plate mantle layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16

rest of cerebellum mantle layer

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16

pons mantle layer

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16

midbrain mantle layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17

facial vii ganglion

strong

regional

strong expression: see section 03 04 05 15 16 18 19

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 16

trigeminal v ganglion

strong

regional

strong expression: see section 02 03 04 05 06 07 15 16 17 18 19 20

vagus x ganglion

strong

regional

strong expression: see section 06 07

spinal cord mantle layer

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 05 06 07 08 09 12 13 14 15

heart ventricle

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30501
Entity Detected:	Camk2d, calcium/calmodulin-dependent protein kinase II, delta ( MGI:1341265)
Sequence:	sense strand is shown >T30501 CAGGGACCTGAAGCCTGAGAATTTGCTTTTAGCTAGCAAGTCCAAAGGAGCAGCTGTGAAGCTGGCAGAC TTCGGCTTAGCCATAGAAGTTCAAGGCGACCAGCAGGCATGGTTTGGTTTTGCTGGCACACCTGGGTATC TTTCTCCAGAAGTCCTGCGTAAAGATCCTTATGGAAAACCAGTGGATATGTGGGCATGCGGTGTCATCCT CTACATCTTGCTGGTGGGATACCCACCCTTCTGGGATGAAGATCAGCATAGACTGTATCAGCAGATCAAG GCCGGAGCTTATGATTTTCCGTCACCAGAATGGGATACAGTGACACCTGAAGCCAAAGACCTCATCAACA AAATGCTGACCATCAACCCTGCCAAACGTATCACAGCCTCTGAGGCCCTGAAACACCCATGGATCTGTCA ACGCTCTACTGTTGCCTCCATGATGCACAGGCAGGAGACTGTAGACTGCTTGAAGAAATTTAATGCTAGA CGGAAACTGAAGGGCGCCATCTTGACAACTATGCTGGCTACGAGAAATTTTTCAGCAGCCAAGAGTTTAT TGAAGAAACCAGATGGGGTAAAGGAGTCAACTGAGAGCTCAAACACCACCATTGAGGATGAAGACGTGAA AGCACGAAAACAGGAGATCATCAAAGTCACTGAGCAACTGATTGAAGCTATCAACAATGGGGACTTTGAG GCTTACACAAAAATCTGTGATCCAGGCCTCACTGCCTTTGAACCTGAAGCATTGGGTAACTTAGTGGAAG GGATGGACTTTCACAGATTCTACTTTGAAAATGCTTTGTCCAAAAGCAATAAACCAATCCACACGATCAT CCTCAACCCACACGTGCA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:30055337), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 58901. Forward Primer - name:058901_F_IRAV108_f11_Camk2d, sequence:CAGGGACCTGAAGCCTGA; Reverse Primer - name:058901_R_SP6_IRAV108_f11_Camk2d, sequence:GTGCACGTGTGGGTTGAG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15324 same embryo
	EMAGE:15327 same embryo
	EMAGE:15325 same embryo
	EMAGE:15323 same embryo
	EMAGE:15328 same embryo
	EurExpress:euxassay_010500 same experiment
	MGI:4823604 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source