EMAGE mouse gene expression spatial database

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EMAGE:15327

Bcat1 branched chain aminotransferase 1, cytosolic ( MGI:104861)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010499. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010499_01	euxassay_010499_02	euxassay_010499_03	euxassay_010499_04

euxassay_010499_05	euxassay_010499_06	euxassay_010499_07	euxassay_010499_08	euxassay_010499_09

euxassay_010499_10	euxassay_010499_11	euxassay_010499_12	euxassay_010499_13	euxassay_010499_14

euxassay_010499_15	euxassay_010499_16	euxassay_010499_17	euxassay_010499_18	euxassay_010499_19

euxassay_010499_20	euxassay_010499_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15327_wholemount_strong.wlz

15327_wholemount_moderate.wlz

15327_wholemount_weak.wlz

15327_wholemount_possible.wlz

15327_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15327_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 01 02 03 04 17 18 19 weak expression: see section 05 06 16

brain

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

facial vii ganglion

moderate

single cell

moderate expression: see section 03 04 05 18

trigeminal v ganglion

moderate

single cell

moderate expression: see section 02 03 04 05 06 07 15 16 17 18 19 20

spinal cord

weak

regional

weak expression: see section 05 06 07 08 09 10 11 12 13 14

neural retina

moderate

regional

moderate expression: see section 01 02 19 20 21

nasal septum

weak

regional

weak expression: see section 09 10 11 12

viscerocranium

weak

regional

Expression in the turbinate bone.

tongue

weak

regional

weak expression: see section 09 10 11 12

mandible

weak

regional

weak expression: see section 02 03 04 05 07 15 16 17 18 19

lower jaw incisor

weak

regional

weak expression: see section 09 10 11 12 13

upper jaw incisor

weak

regional

weak expression: see section 09 12 13

orbito-sphenoid

moderate

regional

moderate expression: see section 21 weak expression: see section 01 02 03 04 05 06 15 16 18 19 20

clavicle

moderate

regional

moderate expression: see section 07 08 09 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30499
Entity Detected:	Bcat1, branched chain aminotransferase 1, cytosolic ( MGI:104861)
Sequence:	sense strand is shown >T30499 TTCGCTGGTCTTTGGAGCTACGTTTACTGACCACATGCTGACGGTGGAGTGGTCCTCTGCGTCTGGATGG GAGAAACCTCACATTAAGCCTTTTGGAAACTTGCCCATACATCCCGCTGCCTCTGTTTTGCACTACGCTG TGGAACTGTTTGAAGGCTTGAAAGCCTTTCGGGGAGTTGATAACAAGATCCGATTGTTCCGGCCGGACCT CAACATGGATAGAATGTGCCGATCTGCTGTGAGGACCACGCTGCCGATGTTTGACAAGGAGGAGCTCCTA AAGTGTATTCTTCAGCTTCTACAGATCGACCAAGAATGGGTTCCCTACTCCACCTCTGCCAGCCTCTACA TCCGCCCCACATTTATCGGAACTGAGCCATCTCTTGGCGTCAAGAAGCCTTCCAAAGCCCTACTCTTTGT GATCCTGAGCCCCGTGGGACCTTATTTTTCTAGTGGATCTTTTACTCCGGTGTCCCTGTGGGCCAATCCA AAGTATATCAGAGCCTGGAAAGGTGGGACTGGAGACTGCAAGATGGGCGGCAATTATGGAGCCTCCCTTC TGGCACAGTGCGAGGCCGCGGAGAATGGCTGTCAGCAGGTCCTGTGGCTGTACGGCAAGGACAACCAGAT AACTGAAGTAGGCACAATGAATCTTTTCCCCTACTGGATAAACGAAGACGGAGAAGAGGAGCTGGCAACG CCCCCACTAGATGGCATCATTCTCCCAGGAGTGACCAGGCAGAGCATCCTGGAGCTGGCACAACAGTGGG GTGAGTTTAAGGTATGTGAGAGACACCTCACCATGGATGACCTGGCCACCGCCCTTGAGGGGAACAGAGT GAAGGAGATGTTCGGCTCAGGGACAGCCTGCGTTGTCTGCCCAGTC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:30063465), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 58795. Forward Primer - name:058795_F_IRAV108_d08_Bcat1, sequence:TTCGCTGGTCTTTGGAGC; Reverse Primer - name:058795_R_SP6_IRAV108_d08_Bcat1, sequence:AGACTGGGCAGACAACGC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15324 same embryo
	EMAGE:15326 same embryo
	EMAGE:15325 same embryo
	EMAGE:15323 same embryo
	EMAGE:15328 same embryo
	EurExpress:euxassay_010499 same experiment
	MGI:4823441 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source