EMAGE mouse gene expression spatial database

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EMAGE:15333

Timm50 translocase of inner mitochondrial membrane 50 homolog (yeast) ( MGI:1913775)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010521. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010521_01	euxassay_010521_02	euxassay_010521_03	euxassay_010521_04

euxassay_010521_05	euxassay_010521_06	euxassay_010521_07	euxassay_010521_08	euxassay_010521_09

euxassay_010521_10	euxassay_010521_11	euxassay_010521_12	euxassay_010521_13	euxassay_010521_14

euxassay_010521_15	euxassay_010521_16	euxassay_010521_17	euxassay_010521_18	euxassay_010521_19

euxassay_010521_20	euxassay_010521_21	euxassay_010521_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15333_wholemount_strong.wlz

15333_wholemount_moderate.wlz

15333_wholemount_weak.wlz

15333_wholemount_possible.wlz

15333_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15333_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

axial musculature

moderate

regional

moderate expression: see section 02 03 18 19

adrenal gland

moderate

regional

moderate expression: see section 05 06 07 15 16

thymus primordium

moderate

regional

moderate expression: see section 09 10 12 13

submandibular gland primordium

moderate

regional

moderate expression: see section 05 06 07 08 15 16 17

thyroid gland

moderate

regional

moderate expression: see section 13 weak expression: see section 14

vibrissa

moderate

regional

moderate expression: see section 03 04 17 18 19

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 06 07 08 15 16 17 weak expression: see section 09 10 13 14

mandible

moderate

regional

moderate expression: see section 02 03 04 06 07 08 09 10 12 13 14 15 16 17 18 19 20 21 weak expression: see section 05

maxilla

moderate

regional

moderate expression: see section 06 07 09 10 13 14 15 16 17

liver

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

metanephros

moderate

regional

moderate expression: see section 05 06 07 08 09 15 16 17 18

lung

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30788
Entity Detected:	Timm50, translocase of inner mitochondrial membrane 50 homolog (yeast) ( MGI:1913775)
Sequence:	sense strand is shown >T30788 GATCATCGAGCCCACCAGTCCCTGCCTTCTCCCAGACCCTCTGCGGGAGCCCTACTACCAGCCACCTTAT ACACTGGTCCTGGAGCTTACCGGGGTCCTTCTGCACCCAGAGTGGTCGCTGGCCACTGGCTGGAGGTTTA AGAAGCGTCCAGGCATCGAGACCTTGTTCCAGCAGCTTGCCCCTCTGTATGAAATAGTCATCTTCACATC AGAGACTGGCATGACTGCATTTCCACTCATTGATAGTGTGGACCCTCACGGTTTCATCTCCTACCGTCTG TTCCGGGACGCCACCAGATATATGGAGGGACATCATGTGAAGGACATCTCCTGTCTGAATCGGGACCCAG CCCGAGTAGTAGTCGTGGACTGCAAGAAGGAAGCTTTCCGCCTCCAGCCCTTCAACGGTGTTGCCCTGCG GCCCTGGGATGGCAACTCAGATGACCGGGTCTTGCTGGACCTGTCTGCCTTCCTCAAGACCATTGCACTG AACCAAGTGGAGGACGTCCGGACGGTGCTGGAGCACTATGCTCTAGAGGATGACCCACTGGAGGCATTCA AACAGCGGCAGAGCCGGCTTGAGCAGGAGGAGCAGCAGCGCCTGGCCGAGCTCTCCAAGTCCAACAGGCA GGGCCTCTCCTTTGGCTCACTCGCCAGCCGACTGTGGCCTCGCTCCAAGCAGCCCTGACTTCTAGGCTTC CTCAGAGACTTGATGCCTGAGCCTTGGGCCCCAGCCCTTACACGGCAAAGTGTGGACAGCTATGTGTCCA ATAAAGCGAGTCCCAGATGCCACAATGCCTGTGTCC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3597676), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 16125. Forward Primer - name:016125_F_IRAV16-19_F02_2810403L02Rik, sequence:GATCATCGAGCCCACCAG; Reverse Primer - name:016125_R_SP6_IRAV16-19_F02_2810403L02Rik, sequence:TGGACACAGGCATTGTGG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15332 same embryo
	EMAGE:15330 same embryo
	EMAGE:15331 same embryo
	EMAGE:15329 same embryo
	EurExpress:euxassay_010521 same experiment
	MGI:4828719 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source