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EMAGE:15466

1810019J16Rik RIKEN cDNA 1810019J16 gene ( MGI:1916323)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15466 EMAGE:15466 EMAGE:15466 EMAGE:15466 EMAGE:15466
"Pseudo-wholemount" of euxassay_010639. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010639_01 euxassay_010639_02 euxassay_010639_03 euxassay_010639_04
EMAGE:15466 EMAGE:15466 EMAGE:15466 EMAGE:15466 EMAGE:15466
euxassay_010639_05 euxassay_010639_06 euxassay_010639_07 euxassay_010639_08 euxassay_010639_09
EMAGE:15466 EMAGE:15466 EMAGE:15466 EMAGE:15466 EMAGE:15466
euxassay_010639_10 euxassay_010639_11 euxassay_010639_12 euxassay_010639_13 euxassay_010639_14
EMAGE:15466 EMAGE:15466 EMAGE:15466 EMAGE:15466 EMAGE:15466
euxassay_010639_15 euxassay_010639_16 euxassay_010639_17 euxassay_010639_18 euxassay_010639_19
EMAGE:15466 EMAGE:15466 EMAGE:15466 EMAGE:15466 EMAGE:15466
euxassay_010639_20 euxassay_010639_21 euxassay_010639_22 euxassay_010639_23 euxassay_010639_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15466Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15466_wholemount_strong.wlz
15466_wholemount_moderate.wlz
15466_wholemount_weak.wlz
15466_wholemount_possible.wlz
15466_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15466_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
thymus primordium
weak weak
regionalweak expression: see section 11 12 13 14 15
submandibular gland primordium
weak weak
regionalweak expression: see section 06 07 08 09 10 18 19 20 21
pancreas
weak weak
regionalweak expression: see section 09 10 11
thyroid gland
weak weak
regionalweak expression: see section 10 11 15 16
pituitary gland
weak weak
regionalweak expression: see section 11 12 13 14 15 16
vibrissa
weak weak
regionalweak expression: see section 06 07 08 22 23 24
lateral ventricle choroid plexus
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13 14 15 weak expression: see section 16 17 18
metencephalon part of 4th ventricle choroid plexus
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13 14 15 weak expression: see section 16 17 18 19
nasal cavity olfactory epithelium
weak weak
regionalweak expression: see section 10 11 12 13 14 16 17 18 19 20
midgut
weak weak
regionalweak expression: see section 12 14 15 16 17
lower jaw incisor
weak weak
regionalweak expression: see section 11 12 13 16 17
lower jaw molar
weak weak
regionalweak expression: see section 08 19
upper jaw incisor
weak weak
regionalweak expression: see section 12 13 16 17
upper jaw molar
weak weak
regionalweak expression: see section 08 20
bladder
weak weak
regionalweak expression: see section 12 13 14
urethra of male
weak weak
regionalweak expression: see section 12 13 14
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30785
Entity Detected:1810019J16Rik, RIKEN cDNA 1810019J16 gene ( MGI:1916323)
Sequence:sense strand is shown

>T30785
TGTCTACCCCTACCGCCATGCCACCTCCCCAGTCCCTGACGTGGACTCCTGCTGCAAGGAGCCCCTGGCC
GAGCCTCCTCCCACACGGCACAGCTTGCCTAGCACCTTCACCAACAGCCCCCGCGGCTCTGAGGAGTACT
ACTCCTTCCATGAATCGGACCTGGACCTGCCTGAGATGGGCAGTGGCTCCATGTCGAGCCGGGAGATCGA
CGTGCTTATTTTCAAGAAGCTGACAGAGCTGTTCAGCGTGCACCAGATTGACGAGCTGGCCAAGTGCACA
TCGGACACCGTGTTCCTGGAGAAGACCAGCAAGATCTCAGACCTGATCAGCAGCATCACGCAGGACTACC
ACCTGGATGAGCAAGACGCCGAGGGCCGCCTGGTGCGGGGCATCATCCGTATTAGTACCCGCAAAAGCCG
CTCCCGCCCACAGACCTCCGAGGGGCGCTCAGCCCGCTCTACTGCCCCTGCTGCTGCCCCCGACAGTGGC
CATGAGACCATGCTGGGCTCTGGCCTCAGCCAGGACGAGCTGACAGTCCAGATCTCCCAGGAGACAACCG
CAGATGCCATCGCCAGGAAACTGAGGCCGTATGGAGCCCCAGGGTACCCAGCCAGCCAAGACTCATCATT
CCAGGGCACGGACACAGACTCTTCAGGGGCACCCCTGCTGCAGGTGTACTGCTAACCCCTACTGGCCCAG
CAGTCACGGCCTCTCCTGGAAGAAGCATAGCCAGCGGAGGAGAGGAACCAGGACCTCTGTGTGGGGCCGC
CTCTGAGCCCAGAAGCAGTGACCTCTCTGGCTTCTGCTTTTGTTGAATCCCCTTAGACCACATACATTTC
ACAGGGCCATGGTGCCCATACCCCCTTCCAGCTGGCCTGACTGCTGGCTGGGCCTCTTCTGCGGCCTGAA
AGCAAAGCCTTGGAGGTAGCTTCCCAGTTCCACTCCC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3599314), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 16205. Forward Primer - name:016205_F_IRAV16-19_E12_1810019J16Rik, sequence:TGTCTACCCCTACCGCCA; Reverse Primer - name:016205_R_SP6_IRAV16-19_E12_1810019J16Rik, sequence:TGGGAGTGGAACTGGGAA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15467 same embryo
 EMAGE:15465 same embryo
 EMAGE:15468 same embryo
 EMAGE:15469 same embryo
 EMAGE:15464 same embryo
 EurExpress:euxassay_010639 same experiment
 MGI:4822637 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS